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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2014 / Cilt: 38 - Sayı: 6

Study of insulin-like growth factor 1 effects on bovine type A spermatogonia proliferation and viability

Babak QASEMI PANAHI
Parviz TAJIK
Mansoureh MOVAHEDIN
Gholamali MOGHADDAM
Mohammad Hossein GERANMAYEH
Sayfa
693–698
Yayın Tarihi
2014-06-01
DOI
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Overall, these data demonstrate that usage of 100 ng/mL IGF-1 in vitro improves proliferation and postfreezing viability rate of SSCs.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds.
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Temel Bulgular & Veri:In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1).
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Sonuç & Katkı:Overall, these data demonstrate that usage of 100 ng/mL IGF-1 in vitro improves proliferation and postfreezing viability rate of SSCs.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Spermatogonial stem cells · proliferation · IGF-1

Özet

Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds. In fact, SSCs transmit genetic information to future generations. In vitro improvement in proliferation and frozen-thawed SSCs numbers is therefore needed. In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1). During 16 days of incubation, 100 ng/mL IGF-1 showed higher efficacy in increasing the numbers and diameters of SSCs colonies than 40 and 150 ng/mL concentrations. Furthermore, 100 ng/mL IGF-1 increased the frozen/thawed cells’ viability rate. Overall, these data demonstrate that usage of 100 ng/mL IGF-1 in vitro improves proliferation and postfreezing viability rate of SSCs.

Abstract

Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds. In fact, SSCs transmit genetic information to future generations. In vitro improvement in proliferation and frozen-thawed SSCs numbers is therefore needed. In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1). During 16 days of incubation, 100 ng/mL IGF-1 showed higher efficacy in increasing the numbers and diameters of SSCs colonies than 40 and 150 ng/mL concentrations. Furthermore, 100 ng/mL IGF-1 increased the frozen/thawed cells’ viability rate. Overall, these data demonstrate that usage of 100 ng/mL IGF-1 in vitro improves proliferation and postfreezing viability rate of SSCs.

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Bu Makaleye Atıf Yap

PANAHI, B. Q., TAJIK, P., MOVAHEDIN, M., MOGHADDAM, G., GERANMAYEH, M. H. (2014). Study of insulin-like growth factor 1 effects on bovine type A spermatogonia proliferation and viability. Turkish Journal of Veterinary and Animal Sciences, Cilt: 38 - Sayı: 6, 693–698. https://ajindex.com/articles/121537

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Kaynakça (10)

  1. Zheng Y, Zhang Y, Qu R, He Y, Tian X, Zeng W. Spermatogonial stem cells from domestic animals: progress and prospects. Reproduction 2014; 147: R65–R74. Dobrinski I. Germ cell transplantation and testis tissue xenografting in domestic animals. Anim Reprod Sci 2005; 89: 137–145.
  2. Nagano M, Brinster CJ, Orwig KE, Ryu BY, Avarbock MR, Brinster RL. Transgenic mice produced by retroviral transduction of male germ-line stem cells. Proc Natl Acad Sci U S A 2001; 98: 13090–13095.
  3. Aponte PM, Soda T, Teerds KJ, Mizrak SC, van de Kant HJG, de Rooij DG. Propagation of bovine spermatogonial stem cells in vitro. Reproduction 2008; 136: 543–557.
  4. Izadyar F, Matthijs-Rijsenbilt JJ, Den Ouden K, Creemers LB, Woelders H, De Rooij DG. Development of a cryopreservation protocol for type A spermatogonia. J Androl 2002; 23: 537– 545. 8. Koruji SM, Movahedin M, Mowla SJ, Gorabi H, Jabari Arfaei A. The effects of inducer factors on adult mouse spermatogonial cells colony formation in vitro. Yakhteh 2007; 9: 141–150.
  5. Rinderknecht E, Humbel RE. The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. J Biol Chem 1978; 253: 2769–2776.
  6. Blundell TL, Humbel RE. Hormone families: pancreatic hormones and homologous growth factors. Nature 1980; 287: 781–787.
  7. Huang YH, Chin CC, Ho NN, Chou CK, Shen CN, Kuo HC, Wu TJ, Wu YC, Hung YC, Chang CC et al. Pluripotency of mouse spermatogonial stem cells maintained by IGF-1- dependent pathway. FASEB J 2009; 23: 2076–2087. Bähr M, Kolter T, Seipke G, Eckel J. Growth promoting and metabolic activity of the human insulin analogue [Gly(A21),Arg(B31),Arg(B32)]insulin (HOE 901) in muscle cells. Eur J Pharmacol 1997; 320: 259–265.
  8. Eckardt K, May C, Koenen M, Eckel J. IGF-1 receptor signalling determines the mitogenic potency of insulin analogues in human smooth muscle cells and fibroblasts. Diabetologia 2007; 50: 2534–2543.
  9. Valentinis B, Morrione A, Peruzzi F, Prisco M, Reiss K, Baserga R. Anti-apoptotic signaling of the IGF-I receptor in fibroblasts following loss of matrix adhesion. Oncogene 1999; 18: 1827– 1836. Kubota H, Avarbock MR, Brinster RL. Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells. Proc Natl Acad Sci U S A 2004; 101: 16489–16494.
  10. Van Pelt AMM, Morena AR, Van Dissel-Emiliani FMF, Boitani C, Gaemers IC, De Rooij DG, Stefanini M. Isolation of the synchronized A spermatogonia from adult vitamin A- deficient rat testes. Biol Reprod 1996; 55: 439–444. Bell