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Journals / Turkish Journal of Veterinary and Animal Sciences / 2014 / Cilt: 38 - Sayı: 6

Study of insulin-like growth factor 1 effects on bovine type A spermatogonia proliferation and viability

Babak QASEMI PANAHI
Parviz TAJIK
Mansoureh MOVAHEDIN
Gholamali MOGHADDAM
Mohammad Hossein GERANMAYEH
Pages
693–698
Publication Date
2014-06-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Overall, these data demonstrate that usage of 100 ng/mL IGF-1 in vitro improves proliferation and postfreezing viability rate of SSCs.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds.
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Key Findings & Data:In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1).
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Conclusion & Impact:Overall, these data demonstrate that usage of 100 ng/mL IGF-1 in vitro improves proliferation and postfreezing viability rate of SSCs.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Spermatogonial stem cells · proliferation · IGF-1

Abstract

Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds. In fact, SSCs transmit genetic information to future generations. In vitro improvement in proliferation and frozen-thawed SSCs numbers is therefore needed. In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1). During 16 days of incubation, 100 ng/mL IGF-1 showed higher efficacy in increasing the numbers and diameters of SSCs colonies than 40 and 150 ng/mL concentrations. Furthermore, 100 ng/mL IGF-1 increased the frozen/thawed cells’ viability rate. Overall, these data demonstrate that usage of 100 ng/mL IGF-1 in vitro improves proliferation and postfreezing viability rate of SSCs.

Özet

Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds. In fact, SSCs transmit genetic information to future generations. In vitro improvement in proliferation and frozen-thawed SSCs numbers is therefore needed. In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1). During 16 days of incubation, 100 ng/mL IGF-1 showed higher efficacy in increasing the numbers and diameters of SSCs colonies than 40 and 150 ng/mL concentrations. Furthermore, 100 ng/mL IGF-1 increased the frozen/thawed cells’ viability rate. Overall, these data demonstrate that usage of 100 ng/mL IGF-1 in vitro improves proliferation and postfreezing viability rate of SSCs.

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Cite This Article

PANAHI, B. Q., TAJIK, P., MOVAHEDIN, M., MOGHADDAM, G., GERANMAYEH, M. H. (2014). Study of insulin-like growth factor 1 effects on bovine type A spermatogonia proliferation and viability. Turkish Journal of Veterinary and Animal Sciences, Cilt: 38 - Sayı: 6, 693–698. https://ajindex.com/articles/121537

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References (10)

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