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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2011 / Cilt: 35 - Sayı: 1

Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro

Yong-Jun LI
Hong AO
Gui-jin SUN
Li ZHANG
Sayfa
15–19
Yayın Tarihi
2011-01-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.

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Amaç & Hipotez:Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened.
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Temel Bulgular & Veri:In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively.
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Sonuç & Katkı:All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Single preimplantation embryo mRNA differential display · goat · embryo

Özet

Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.

Abstract

Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.

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Bu Makaleye Atıf Yap

LI, Y., AO, H., SUN, G., ZHANG, L. (2011). Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro. Turkish Journal of Veterinary and Animal Sciences, Cilt: 35 - Sayı: 1, 15–19. https://ajindex.com/articles/121515

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Kaynakça (8)

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