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Journals / Turkish Journal of Veterinary and Animal Sciences / 2011 / Cilt: 35 - Sayı: 1

Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro

Yong-Jun LI
Hong AO
Gui-jin SUN
Li ZHANG
Pages
15–19
Publication Date
2011-01-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened.
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Key Findings & Data:In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively.
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Conclusion & Impact:The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Single preimplantation embryo mRNA differential display · goat · embryo

Abstract

Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.

Özet

Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.

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Cite This Article

LI, Y., AO, H., SUN, G., ZHANG, L. (2011). Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro. Turkish Journal of Veterinary and Animal Sciences, Cilt: 35 - Sayı: 1, 15–19. https://ajindex.com/articles/121515

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