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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2014 / Cilt: 38 - Sayı: 5

The midgut bacterial flora of the hard tick Hyalomma anatolicum (Acari: Ixodidae) from South India as determined by molecular analyses

Sankarappan ANBALAGAN
Vimalanathan ARUN PRASANNA
Mani KANNAN
Muthukalingan KRISHNAN
Sayfa
520–525
Yayın Tarihi
2014-05-01
DOI
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Further study is needed to understand the relationship between midgut bacterial community and tick-pathogen interaction.

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Amaç & Hipotez:The midgut bacterial flora of post-blood meal and starved or digested blood meal of hard ticks (Hyalomma anatolicum) from goats were analyzed using BOX PCR, ERIC PCR and 16S rDNA gene sequencing.
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Temel Bulgular & Veri:In total, 9 bacterial species belonging to 2 genera (Bacillus and Staphylococcus) were identified in the midgut of both types (post-blood meal and digested blood meal) of tick by using 16S rDNA gene sequencing.
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Sonuç & Katkı:Further study is needed to understand the relationship between midgut bacterial community and tick-pathogen interaction.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Midgut bacterial flora · Hyalomma anatolicum · 16S rDNA

Özet

The midgut bacterial flora of post-blood meal and starved or digested blood meal of hard ticks (Hyalomma anatolicum) from goats were analyzed using BOX PCR, ERIC PCR and 16S rDNA gene sequencing. BOX PCR and ERIC PCR showed dissimilar band patterns between microbial isolates from the midgut of the ticks. In total, 9 bacterial species belonging to 2 genera (Bacillus and Staphylococcus) were identified in the midgut of both types (post-blood meal and digested blood meal) of tick by using 16S rDNA gene sequencing. The majority of the midgut bacterial species were Staphylococcus sp. (90%) and there was a high frequency of 3 species of Staphylococcus (S. chromogenes, S. epidermidis, and S. gallinarum). Phylogenetic analysis based on 16S rDNA gene sequences showed that all midgut isolates were monophyletic and showed high-frequency relationships with S. chromogenes and S. epidermidis. Further study is needed to understand the relationship between midgut bacterial community and tick-pathogen interaction.

Abstract

The midgut bacterial flora of post-blood meal and starved or digested blood meal of hard ticks (Hyalomma anatolicum) from goats were analyzed using BOX PCR, ERIC PCR and 16S rDNA gene sequencing. BOX PCR and ERIC PCR showed dissimilar band patterns between microbial isolates from the midgut of the ticks. In total, 9 bacterial species belonging to 2 genera (Bacillus and Staphylococcus) were identified in the midgut of both types (post-blood meal and digested blood meal) of tick by using 16S rDNA gene sequencing. The majority of the midgut bacterial species were Staphylococcus sp. (90%) and there was a high frequency of 3 species of Staphylococcus (S. chromogenes, S. epidermidis, and S. gallinarum). Phylogenetic analysis based on 16S rDNA gene sequences showed that all midgut isolates were monophyletic and showed high-frequency relationships with S. chromogenes and S. epidermidis. Further study is needed to understand the relationship between midgut bacterial community and tick-pathogen interaction.

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Bu Makaleye Atıf Yap

ANBALAGAN, S., PRASANNA, V. A., KANNAN, M., KRISHNAN, M. (2014). The midgut bacterial flora of the hard tick Hyalomma anatolicum (Acari: Ixodidae) from South India as determined by molecular analyses. Turkish Journal of Veterinary and Animal Sciences, Cilt: 38 - Sayı: 5, 520–525. https://ajindex.com/articles/121464

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Kaynakça (10)

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