KaydedilenlerDerginizi ekleyin

Dergiler / Kocatepe Veteriner Dergisi / 2024 / Cilt: 17 Sayı: 4

Sıçanlarda Glifosat İzopropilamin ile Oluşturulan Testiküler Hasara Karşı Klorojenik Asidin Koruyucu Etkisi

Protective Effect of Chlorogenic Acid Against Testicular Damage Induced By Glyphosate Isopropilamine in Rats

Ruhi Türkmen(Afyon Kocatepe Üniversitesi Veteriner Fakültesi)0000-0003-4726-3900
Yavuz Osman Birdane(AFYON KOCATEPE UNIVERSITY, FACULTY OF VETERINARY MEDICINE)0000-0003-0754-7909
Orkun Atik(AFYON KOCATEPE UNIVERSITY, FACULTY OF VETERINARY MEDICINE)0000-0003-2411-7492
Hasan Hüseyin Demirel(AFYON KOCATEPE UNIVERSITY, BAYAT VOCATIONAL SCHOOL)0000-0002-4795-2266
Türkan Türkmen(AFYON KOCATEPE UNIVERSITY, INSTITUTE OF HEALTH SCIENCES)0000-0003-0736-8927
Özay Güleş(AYDIN ADNAN MENDERES UNIVERSITY, FACULTY OF VETERINARY MEDICINE)0000-0001-6170-1706
Sayfa
334–340
Yayın Tarihi
2025-01-03
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Sonuçlar, GLF-ISO'nun neden olduğu testis hasarının CGA ile potansiyel olarak önlenebileceğini veya yönetilebileceğini göstermektedir.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Bu subkronik toksisite çalışmasının amacı, testis dokularında Glifosat izopropilamin (GLF-ISO) tarafından indüklenen histopatolojik-histolojik değişiklikler ve oksidatif stres üzerinde klorojenik asidin (CGA) profilaktik etkisini belirlemektir.
📊
Temel Bulgular & Veri:Toplam 42 erkek Wistar sıçan, her biri 7 sıçan içeren altı eşit gruba ayrılmıştır.
💡
Sonuç & Katkı:Sonuçlar, GLF-ISO'nun neden olduğu testis hasarının CGA ile potansiyel olarak önlenebileceğini veya yönetilebileceğini göstermektedir.
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Glyphosate İzopropilamin · Klorojenik asit · Oksidatif stres

Özet

Bu subkronik toksisite çalışmasının amacı, testis dokularında Glifosat izopropilamin (GLF-ISO) tarafından indüklenen histopatolojik-histolojik değişiklikler ve oksidatif stres üzerinde klorojenik asidin (CGA) profilaktik etkisini belirlemektir. Toplam 42 erkek Wistar sıçan, her biri 7 sıçan içeren altı eşit gruba ayrılmıştır. Ön muamele için sıçanlara 7 hafta boyunca 12.5, 25 ve 50 mg/kg (po) dozlarında CGA ve 787.85 mg/kg (po) dozunda GLF-ISO verilmiştir. GLF-ISO testis dokularında malondialdehit seviyelerini önemli ölçüde artırırken SOD ve CAT aktivitelerini ve GSH seviyelerini azaltmıştır. Aksine, bu parametreler CGA ile tedavi edilen gruplarda iyileşmiştir. Ayrıca, CGA, GLF-ISO tarafından indüklenen testis dokularındaki histopatolojik ve histolojik değişiklikleri doza bağlı bir şekilde iyileştirmiştir. Sonuçlar, GLF-ISO'nun neden olduğu testis hasarının CGA ile potansiyel olarak önlenebileceğini veya yönetilebileceğini göstermektedir.

Abstract

The aim of this subchronic toxicity study was to determine the prophylactic effect of chlorogenic acid (CGA) on the histopathologic-histologic changes and oxidative stress induced by Glyphosate isopropylamine (GLF-ISO) in testicular tissues. A sum of 42 male Wistar rats were divided into six equal groups, each containing 7 rats. For pretreatment, rats were given CGA at doses of 12.5, 25 and 50 mg/kg (po) and GLF-ISO at 787.85 mg/kg (po) for 7 weeks. GLF-ISO significantly increased malondialdehyde levels while decreasing SOD and CAT activities and GSH levels in testicular tissues. On the contrary, these parameters were improved in CGA-treated groups. Furthermore, CGA ameliorated the histopathological and histological changes in testicular tissues induced by GLF-ISO in a dose-dependent manner. The results indicate that testicular damage caused by GLF-ISO can potentially be prevented or managed by CGA.

🔖

Bu Makaleye Atıf Yap

Türkmen, R., Birdane, Y. O., Atik, O., Demirel, H. H., Türkmen, T., Güleş, Ö. (2025). Sıçanlarda Glifosat İzopropilamin ile Oluşturulan Testiküler Hasara Karşı Klorojenik Asidin Koruyucu Etkisi. Kocatepe Veteriner Dergisi, Cilt: 17 Sayı: 4, 334–340. https://doi.org/10.30607/kvj.1520615

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (30)

  1. Aebi, H. (1984). Catalase in vitro. In Methods in Enzymology (Vol. 105, pp. 121-126). Academic press Available from: http://www.ncbi.nlm.nih.gov/pubmed/6727660
  2. Ahbab, M.A., Barlas, N., & Karabulut, G. (2017). The toxicological effects of bisphenol A and octylphenol on the reproductive system of prepubertal male rats. Toxicol Ind Health., 33(2): 133–46.
  3. Avdatek, F., Birdane, Y.O., Türkmen, R., & Demirel, H.H. (2018). Ameliorative effect of resveratrol on testicular oxidative stress, spermatological parameters and DNA damage in glyphosate-based herbicide-exposed rats. Andrologia, 50(7): e13036.
  4. Avdatek, F., Kirikkulak, M., & Yeni, D. (2023). The effects of proanthocyanidin on testicular toxicity in rats exposed to a glyphosate-based herbicide. Pol J Vet Sci., 657–66.
  5. Beutler, E., Duron, O., & Kelly, B.M. (1963). Improved method for the determination of blood glutathione. J Lab Clin Med [Internet], 61:882–8. Available from: http://www.ncbi.nlm.nih.gov/pubmed/13967893
  6. Bhardwaj, J.K., Kumar, V., Saraf, P., Panchal, H., Rathee, V., & Sachdeva, S.N. (2022). Efficacy of N-acetyl- l-cysteine against glyphosate induced oxidative stress and apoptosis in testicular germ cells preventing infertility. J Biochem Mol Toxicol., 36(4): e22979.
  7. Clair, É., Mesnage, R., Travert, C., & Séralini, G.É. (2012). A glyphosate-based herbicide induces necrosis and apoptosis in mature rat testicular cells in vitro, and testosterone decrease at lower levels. Toxicology in Vitro. 26(2): 269–79.
  8. Culling, C.F.A., Allison, R.T., & Barr, W.T. (1984). Cellular pathology technique. Butterworths, London Dai, P., Hu, P., Tang, J., Li, Y., & Li, C. (2016). Effect of glyphosate on reproductive organs in male rat. Acta Histochem., 118(5): 519–26.
  9. Hanson, B. (2024). Glyphosate formulations - what’s the diff (and what’s a salt)? [Internet]. 2024 [cited 2024 Jul 17]. Available from: https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=25903
  10. Hashim, A.R., Bashir, D.W., Yasin, N.A.E., Rashad, M.M., & El-Gharbawy, S.M. (2022). Ameliorative effect of N-acetylcysteine on the testicular tissue of adult male albino rats after glyphosate-based herbicide exposure. J Biochem Mol Toxicol., 36(4): e22997.
  11. Hess, R.A. (1990). Quantitative and qualitative characteristics of the stages and transitions in the cycle of the rat seminiferous epithelium: light microscopic observations of perfusion-fixed and plastic-embedded testes. Biol Reprod., 43(3): 525–42.
  12. Jia, Z., Liu, S., Chen, T., Shi, Z., Li, X., Gao, Z., Zhang, Q., & Zhong, C. (2024). Chlorogenic acid can improve spermatogenic dysfunction in rats with varicocele by regulating mitochondrial homeostasis and inhibiting the activation of NLRP3 inflammasomes by oxidative mitochondrial DNA and cGAS/STING pathway. Bioorg Chem., 150: 107571.
  13. Juárez‐Rojas, L., Casillas, F., López, A., Betancourt, M., Ommati, M.M., & Retana‐Márquez, S. (2022). Physiological role of reactive oxygen species in testis and epididymal spermatozoa. Andrologia, 54(4).
  14. Leblond, C.P., & Clermont, Y. (1952). Definition of the stages of the cycle of the seminiferous epithelium in the rat. Ann N Y Acad Sci., 55(4): 548–73.
  15. Liu, J.B., Li, Z.F., Lu, L., Wang, Z.Y., & Wang, L. (2022). Glyphosate damages blood-testis barrier via NOX1-triggered oxidative stress in rats: Long-term exposure as a potential risk for male reproductive health. Environ Int., 159: 107038.
  16. Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. J Biol Chem., 193(1): 265–75.
  17. Luna, L.G. (1968). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3rd ed. New York,: Blakiston Division, McGraw-Hill.
  18. Nardi, J., Morasi, P.B., Koeppe, C., Dallegrave, E., Leal, M.B., & Rossato-Grando, L.G. (2017). Prepubertal subchronic exposure to soy milk and glyphosate leads to endocrine disruption. Food Chem Toxicol., 100: 247–52.
  19. National Pesticide International Center (2024). http://npic.orst.edu/factsheets/archive/glyphotech.html [Internet]. Glyphosate Technical Fact Sheet. [cited 2024 Jul 17]. Available from: http://npic.orst.edu/factsheets/archive/glyphotech.html
  20. Ohkawa, H., Ohishi, N., & Yagi K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem., 95(2): 351–8.
  21. Owagboriaye, F.O., Dedeke, G.A., Ademolu, K.O., Olujimi, O.O., Ashidi, J.S., & Adeyinka, A.A. (2017). Reproductive toxicity of Roundup herbicide exposure in male albino rat. Exp Toxicol Pathol., 69(7): 461–8.
  22. Pavuluri, H., Bakhtiary, Z., Panner Selvam, M.K., & Hellstrom, W.J.G. (2024). Oxidative Stress-Associated Male Infertility: Current Diagnostic and Therapeutic Approaches. Medicina (Baires), 60(6): 1008.
  23. Qi W, Zhao T, Yang W-W, Wang G-H, Yu H, Zhao H-X, Yang C, Sun L-X. (2011). Comparative pharmacokinetics of chlorogenic acid after oral administration in rats. J Pharm Anal., 1(4): 270–4.
  24. Sharma, R., Agarwal, A., Mohanty, G., Du Plessis, S.S., Gopalan, B., Willard, B., Yadav, S.P., & Sabanegh, E. (2013). Proteomic analysis of seminal fluid from men exhibiting oxidative stress. Reproductive Biology and Endocrinology, 11(1): 85.
  25. Spadella, M.A., Silva, E.J.R., Chies, A.B., & Almeida, L.A. (2024). Insights Into Antioxidant Strategies to Counteract Radiation-Induced Male Infertility. Antioxid Redox Signal, 40(13–15): 776–801.
  26. Sun, Y., Oberley, L.W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clin Chem., 34(3): 497–500.
  27. Tajik, N., Tajik, M., Mack, I., & Enck, P. (2017). The potential effects of chlorogenic acid, the main phenolic components in coffee, on health: a comprehensive review of the literature. Eur J Nutr., 56(7): 2215–44.
  28. Türk, G., Ceribaşı, A.O., Sahna, E., & Ateşşahin, A. (2011). Lycopene and ellagic acid prevent testicular apoptosis induced by cisplatin. Phytomedicine, 18(5): 356–61.
  29. Turkmen, R., & Dogan, I. (2020). Determination of acute oral toxicity of glyphosate isopropylamine salt in rats. Environ Sci Pollut Res Int., 27(16): 19298–303.
  30. Uz, E., Söğüt, S., Sahin, S., Var, A., Ozyurt, H., Güleç, M., & Akyol, O. (2002). The protective role of caffeic acid phenethyl ester (CAPE) on testicular tissue after testicular torsion and detorsion. World J Urol., 20(4): 264–70. Wikipedia (2024). Glyphosate [Internet]. 2024 [cited 2024 Jul 17]. Available from: https://en.wikipedia.org/wiki/Glyphosate