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Dergiler / Türkiye Entomoloji Dergisi / 2026 / Cilt: 49 Sayı: 3

Acı badem yan ürünlerinin biyoaktif potansiyeli ve Ditylenchus dipsaci (Kühn, 1857) (Rhabditida: Anguinidae)’ye karşı nematisidal aktivitesi

Bioactive potential of bitter almond by-products and its nematicidal activity against Ditylenchus dipsaci (Kühn, 1857) (Rhabditida: Anguinidae)

Mehmet Ali Temiz(KARAMANOĞLU MEHMETBEY ÜNİVERSİTESİ)0000-0002-4680-3023
Eşref Can(Karamanoğlu Mehmetbey Üniversitesi)0009-0001-4497-2528
Elif Yavuzaslanoğlu(Karamanoğlu Mehmetbey Üniversitesi)0000-0002-5836-7011
Gamze Aksay(Karamanoğlu Mehmetbey Üniversitesi)0000-0002-6363-1905
Sayfa
307–320
Yayın Tarihi
2025-09-30
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bu çalışma nematisidal aktivitenin anlaşılması için ileride yapılacak çalışmalara önemli bilimsel bilgiler sağlayabilir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Bitki paraziti nematodlar büyük ekonomik kayıplara yol açarken, bitki bazlı doğal ürünlerle entegre zararlı yönetimi umut vadetmektedir.
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Temel Bulgular & Veri:Bu çalışma, 2023 yılında Karaman’dan toplanan acı badem yan ürünlerinin konvansiyonel ve ultrasonik destekli ekstraksiyon yöntemlerle elde edilen ekstraktlarda amigdalin miktarını, ekstraksiyon verimini ve antioksidan kapasitesini belirlemeyi amaçlamaktadır.
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Sonuç & Katkı:Bu çalışma nematisidal aktivitenin anlaşılması için ileride yapılacak çalışmalara önemli bilimsel bilgiler sağlayabilir.
🔬Tür:Araştırma Makalesi
📍Kapsam:2023 Verileri
⚙️Değişkenler:Amigdalin · acı badem · yan ürün

Özet

Bitki paraziti nematodlar büyük ekonomik kayıplara yol açarken, bitki bazlı doğal ürünlerle entegre zararlı yönetimi umut vadetmektedir. Acı bademler, hidrolize edildiğinde toksik hidrojen siyanür (HCN) açığa çıkaran amigdalin molekülünü içerir. Bu çalışma, 2023 yılında Karaman’dan toplanan acı badem yan ürünlerinin konvansiyonel ve ultrasonik destekli ekstraksiyon yöntemlerle elde edilen ekstraktlarda amigdalin miktarını, ekstraksiyon verimini ve antioksidan kapasitesini belirlemeyi amaçlamaktadır. Ayrıca ektraktların ve saf amigdalinin soğan sak nematodu Ditylenchus dipsaci (Kühn, 1857) (Rhabditida: Anguinidae)’ye karşı inhibe edici etkisi ve EC50 değerlerini tespit etmeyi amaçlamaktadır. Ultrasonik destekli ekstraksiyon, konvensiyonel ekstraksiyona göre daha fazla amigdalin geri kazanımı sağlamıştır. Ayrıca, elde edilen bu ekstrakt daha güçlü antioksidan aktivite göstermiştir. Ultrasonik destekli ekstrakt (BAU) 1 mg·mL-1 konsantrasyonda (%90.38±3.70) ve konvansiyonel ekstrakt (BAC) 2 mg·mL-1 konsantrasyonda (%93.46±1.14), fenamifos’dan daha güçlü aktivite (%77.00±2.60) göstermiştir. Saf amigdalin sınırlı nematisidal aktivite (% 26.83±6.94) gösterirken, her iki ekstrakt da daha etkili (2 mg·mL-1 konsantrasyonda BAC ve BAU sırasıyla %93.46±1.14 ve %94.21±0.91) nematisidal aktiviteye sahiptir. Sonuçlar, acı badem yan ürünleri ekstraktlarının bitki koruma ürünleri olarak D. dipsaci’nin yönetiminde umut verici bir potansiyele sahip olduğunu göstermiştir. Bu çalışma nematisidal aktivitenin anlaşılması için ileride yapılacak çalışmalara önemli bilimsel bilgiler sağlayabilir.

Abstract

While plant-parasitic nematodes cause significant economic losses, integrated pest management using plant-based natural products shows promise. Bitter almonds contain the amygdalin molecule, which releases toxic hydrogen cyanide (HCN) when hydrolysed. This study aims to determine the amygdalin content, extraction yield, and antioxidant capacity in extracts obtained using conventional and ultrasonic-assisted extraction methods from bitter almond by-products collected in Karaman in 2023. Additionally, it aims to determine the inhibitory effect of the extracts and authentic amygdalin against the stem and bulb nematode Ditylenchus dipsaci (Kühn, 1857) (Rhabditida: Anguinidae) and their EC50 values. Ultrasonic-assisted extraction recovered more amygdalin than conventional extraction. Furthermore, the extract demonstrated stronger antioxidant activity. Ultrasonic-assisted extract (BAU) at 1 mg·mL-1 concentration (90.38±3.70%) and conventional extract (BAC) at 2 mg·mL-1 concentration (93.46±1.14%) (p<0.05) showed stronger activity than fenamiphos (77.00±2.60%). Authentic amygdalin showed limited nematicidal activity (26.83±6.94%), while both extracts were found to have more potent nematicidal activity (BAC and BAU 2 mg·mL-1 concentration, 93.46±1.14% and 94.21±0.91%, respectively). The results showed that bitter almond by-products extracts have promising potential in the management of D. dipsaci as plant protection products. This study may provide important scientific information for future studies to understand nematicidal activity.

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Bu Makaleye Atıf Yap

Temiz, M. A., Can, E., Yavuzaslanoğlu, E., Aksay, G. (2025). Acı badem yan ürünlerinin biyoaktif potansiyeli ve Ditylenchus dipsaci (Kühn, 1857) (Rhabditida: Anguinidae)’ye karşı nematisidal aktivitesi. Türkiye Entomoloji Dergisi, Cilt: 49 Sayı: 3, 307–320. https://doi.org/10.16970/entoted.1737000

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Kaynakça (50)

  1. Abbott, W. S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economical Entomology, 18 (2): 265-267.
  2. Abid, M. F. & F. Hussain, 2022. Screening of ethnomedicinal plants for their antifungal and nematicidal activities against soil-borne phytopathogens. South African Journal of Botany, 147 (2022): 18-23.
  3. Aydın, Ç. M., Ö. Çelikbıçak & A. A. Hayaloğlu, 2024. Evaluation of antioxidant, antimicrobial, and bioactive properties and peptide sequence composition of Malatya apricot kernels. Journal of the Science of Food and Agriculture, 104 (13): 8022-8036.
  4. Barbosa, P., J. M. Faria, T. Cavaco, A. C. Figueiredo, M. Mota & C. S. Vicente, 2024. Nematicidal activity of phytochemicals against the root-lesion nematode Pratylenchus penetrans. Plants, 13 (5): 726 (1-15).
  5. Chen, Y., A. A. Al-Ghamdi, M. S. Elshikh, M. H. Shah, M. A. Al-Dosary & A. M. Abbasi, 2020. Phytochemical profiling, antioxidant and HepG2 cancer cells’ antiproliferation potential in the kernels of apricot cultivars. Saudi Journal of Biological Sciences, 27 (1): 163-172.
  6. Chitwood, D. J., 2002. Phytochemical based strategies for nematode control. Annual Review of Phytopathology, 40 (1): 221-249.
  7. Cooper, C. E. & G. C. Brown, 2008. The inhibition of mitochondrial cytochrome oxidase by the gases carbon monoxide, nitric oxide, hydrogen cyanide and hydrogen sulfide: chemical mechanism and physiological significance. Journal of Bioenergetics and Biomembranes, 40 (5): 533-539.
  8. Cortés, V., P. Talens, J. M. Barat & M. J. Lerma-García, 2019. Discrimination of intact almonds according to their bitterness and prediction of amygdalin concentration by Fourier transform infrared spectroscopy. Postharvest Biology and Technology, 148 (2019): 236-241.
  9. European Commission, 2022. Green Deal: pioneering proposals to restore Europe’s nature by 2050 and halve pesticide use by 2030. Brussels, Belgium, European Commission. (Web page: https://ec.europa.eu/commission/presscorner/detail/en/ip_22_3746) (Date accessed: 14.08.2025)
  10. García, A. V., A. Beltrán Sanahuja & M. D. C. Garrigós Selva 2013. Characterization and classification of almond cultivars by using spectroscopic and thermal techniques. Journal of Food Science, 78 (2): C138-C144.
  11. Hikal, W. M., R. S. Baeshen, & H. A. Said-Al Ahl, 2017. Botanical insecticide as simple extractives for pest control. Cogent Biology, 3 (1): 1404274 (1-16).
  12. Kepenekci, I., D. Erdoğuş & P. Erdoğan, 2016. Effects of some plant extracts on root-knot nematodes in vitro and in vivo conditions. Turkish Journal of Entomology, 40 (1): 3-14.
  13. Keser, A., E. Demir & O. Yilmaz, 2014. Some bioactive compounds and antioxidant activities of the bitter. Journal of The Chemical Society of Pakistan, 36 (5): 922-930.
  14. Khairy, D. & R. H. El-Mohamady, 2024. Aqueous, ethanolic and nanoparticles of two plant extracts compared to abamectin on tomato plant as affected by Meloidogyne incognita and Rotylenchulus reniformis Infection Egyptian Journal of Agronematology, 23 (2): 25-37.
  15. Khan, M. & A. U. Khan, 2021. Plant parasitic nematodes effectors and their crosstalk with defense response of host plants: A battle underground. Rhizosphere, 17 (2021): 100288 (1-9).
  16. Kumari, B., B. K. Tiwari, M. B. Hossain, N. P. Brunton & D. K. Rai, 2018. Recent advances on application of ultrasound and pulsed electric field technologies in the extraction of bioactives from agro-industrial by-products. Food and Bioprocess Technology, 11 (2): 223-241.
  17. Lee, J., G. Zhang, E. Wood, C. Rogel Castillo & A.E. Mitchell, 2013. Quantification of amygdalin in nonbitter, semibitter, and bitter almonds (Prunus dulcis) by UHPLC- (ESI) QqQ MS/MS. Journal of Agricultural and Food Chemistry, 61 (32): 7754-7759.
  18. Makovi, C. M., C. H. Parker & K. Zhang, 2023. Determination of amygdalin in apricot kernels and almonds using LC-MS/MS. Journal of AOAC International, 106 (2): 457-463.
  19. Ngegba, P. M., G. Cui, M. Z. Khalid & G. Zhong, 2022. Use of botanical pesticides in agriculture as an alternative to synthetic pesticides. Agriculture, 12 (5): 600 (1-24).
  20. Okumuş, E., 2023. Effect of different drying processes on antioxidant and antidiabetic properties of pomegranate press wastes. Yuzuncu Yil University Journal of the Institute of Natural and Applied Sciences 28 (1): 113-120.
  21. Okumus, E. & M. A. Temiz, 2025. Hazelnut by-products as a valuable resource: Lipid peroxidation inhibition effect, bioaccessibility and antidiabetic properties. Food Bioscience, 65 (2025): 106018 (1-10).
  22. Oliveira, I. A. S. Meyer, S. Afonso, A. Sequeira, A. Vilela, P. Goufo, H. Trindade & B. Gonçalves, 2020. Effects of different processing treatments on almond (Prunus dulcis) bioactive compounds, antioxidant activities, fatty acids, and sensorial characteristics. Plants, 9 (11): 1627 (1-18).
  23. Özcan, M. M., 2023. A review on some properties of almond: impact of processing, fatty acids, polyphenols, nutrients, bioactive properties, and health aspects. Journal of Food Science and Technology, 60 (5): 1493-1504.
  24. Paventi, G., C. Di Martino, F. Coppola & M. Iorizzo, 2025. β-Glucosidase activity of Lactiplantibacillus plantarum: A key player in food fermentation and human health. Foods, 14 (9): 1451 (1-28).
  25. Pavlović, N., S. Vidović, J. Vladić, L. Popović, T. Moslavac, S. Jakobović & S. Jokić, 2018. Recovery of tocopherols, amygdalin, and fatty acids from apricot kernel oil: Cold pressing versus supercritical carbon dioxide. European Journal of Lipid Science and Technology, 120 (11): 18000438 (1-8).
  26. Ponomareva, E., A. Badiss, T. Sultana, Q. Yu & H. D. Nguyen, 2022. Genome characterization and development of real-time PCR assays for Ditylenchus dipsaci and D. weischeri. Journal of Nematology, 54 (1): 20220058 (1-12).
  27. Pyo, Y. H., T. C. Lee, L. Logendra & R. T. Rosen, 2004. Antioxidant activity and phenolic compounds of Swiss chard (Beta vulgaris subspecies cycla) extracts. Food chemistry, 85 (1): 19-26.
  28. Quah, Y., S. J. Lee, E. B. Lee, B. T. Birhanu, M. S. Ali, M. A. Abbas, N. Boby, Z. U. Im & S. C. Park, 2020. Cornus officinalis ethanolic extract with potential anti-allergic, anti-inflammatory, and antioxidant activities. Nutrients, 12 (11): 1-13.
  29. Rafiee, F., H. Charehgani & M. Abdollahi, 2019. Evaluation of burdock and mountain almond leaf extracts against Meloidogyne javanica on tomato. Archives of Phytopathology and Plant Protection, 52 (11-12): 1035-1047.
  30. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang & C. Rice-Evans, 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26 (9-10): 1231-123.
  31. Riemann, F. & E. Helmke, 2002. Symbiotic relations of sediment‐agglutinating nematodes and bacteria in detrital habitats: the enzyme‐sharing concept. Marine Ecology, 23 (2): 93-113.
  32. Sasanelli, N., A. Konrat, V. Migunova, I. Toderas, E. Iurcu-Straistaru, S. Rusu, A. Bivol, C. Andoni & P. Veronico, 2021. Review on Control Methods against Plant Parasitic Nematodes Applied in Southern Member States (C Zone) of the European Union. Agriculture, 11 (7): 602 (1-19).
  33. Shim, S. M. & H. Kwon, 2010. Metabolites of amygdalin under simulated human digestive fluids. International Journal of Food Sciences and Nutrition, 61 (8): 770-779.
  34. Siddiqui, A. A., P. S. S. Khaki, A. Sohail, T. Sarwar & B. Bano, 2016. Isolation and purification of phytocystatin from almond: biochemical, biophysical, and immunological characterization. Cogent Biology, 2 (1): 1262489 (1-17).
  35. Sikora, R. A., L. P. G. Molendijk & J. Desaeger, 2022. “Integrated nematode Management and Crop Health: Future Challenges and Opportunities, 3-10”. In: Integrated Nematode Management: State-of-the-Art and Visions for the Future (Eds. R. A. Sikora, L. P. G. Molendijk & J. Desaeger). CABI International, UK, 483 pp.
  36. Singleton, V. L., R. Orthofer & R. M. Lamuela-Raventós, 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299: 152-178.
  37. Sithole, N. T., M. G. Kulkarni, J. F. Finnie & J. Van Staden, 2021. Potential nematicidal properties of plant extracts against Meloidogyne incognita. South African Journal of Botany, 139 (2021): 409-417.
  38. Subbotin, S. A., M. Madani, E. Krall, D. Sturhan & M. Moens, 2005. Molecular diagnostics, taxonomy, and phylogeny of the stem nematode Ditylenchus dipsaci species complex based on the sequences of the internal transcribed spacer-rDNA. Phytopathology, 95 (11): 1308-1315.
  39. Summo, C., M. Palasciano, D. De Angelis, V. M. Paradiso, F. Caponio & A. Pasqualone, 2018. Evaluation of the chemical and nutritional characteristics of almonds (Prunus dulcis (Mill). DA Webb) as influenced by harvest time and cultivar. Journal of the Science of Food and Agriculture, 98 (15): 5647-5655.
  40. Temiz, M. A. & A. Temur, 2017. Effect of solvent variation on polyphenolic profile and total phenolic content of olive leaf extract. Yuzuncu Yıl University Journal of Agricultural Sciences, 27 (1): 43-50.
  41. Temiz, M. A., 2021. Antioxidant and antihyperglycemic activities of Scorzonera cinerea radical leaves in streptozocin-induced diabetic rats. Acta Pharmaceutica, 71 (4): 603-617.
  42. Temiz, M. A., G. Aksay, A. Özcan & E. Yavuzaslanoğlu, 2025. A comparison of extraction methods for preparation of glucosinolate-containing extracts from Brassica juncea antagonistic toward Ditylenchus dipsaci. Nematology, 27 (1): 111-123.
  43. Valdés, A., L. Vidal, A. Beltrán, A. Canals & M.C. Garrigós, 2015. Microwave-assisted extraction of phenolic compounds from almond skin byproducts (Prunus amygdalus): a multivariate analysis approach. Journal of Agricultural and Food Chemistry, 63 (22): 5395-5402.
  44. Wahab, M. F., Z. S. Breitbach, D. W. Armstrong, R. Strattan & A. Berthod, 2015. Problems and pitfalls in the analysis of amygdalin and its epimer. Journal of Agricultural and Food Chemistry, 63 (40): 8966-8973.
  45. Wang, B., T. Pandey, Y. Long, S. E. Delgado-Rodriguez, M. D. Daugherty & D. K. Ma, 2022. Co-opted genes of algal origin protect C. elegans against cyanogenic toxins. Current Biology, 32 (22): 4941-4948.
  46. Xu, S., X. Xu, S. Yuan, H. Liu, M. Liu, Y. Zhang, H. Zhang, Y. Gao, R. Lin & X. Li, 2017. Identification and analysis of amygdalin, neoamygdalin and amygdalin amide in different processed bitter almonds by HPLC-ESI-MS/MS and HPLC-DAD. Molecules, 22 (9): 1425 (1-10).
  47. Yavuzaslanoglu, E. & G. Aksay, 2021. Susceptibility of different plant species to two populations of Ditylenchus dipsaci Kühn, 1857 (Tylenchida: Anguinidae) from Turkey. Turkish Journal of Entomology, 45 (1): 77-86.
  48. Zhang, L., Q. Fu, W. Li, B. Wang, X. Yin, S. Liu, Z. Xu & Q. Niu, 2017. Identification and characterization of a novel β-glucosidase via metagenomic analysis of Bursaphelenchus xylophilus and its microbial flora. Scientific Reports, 7 (1): 14850 (1-11).
  49. Zhishen, J., T. Mengcheng & W. Jianming, 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64 (4): 555-559.
  50. Zia, S., M. R. Khan, M. A. Shabbir, A. Aslam Maan, M. K. I. Khan, M. Nadeem, A. A. Khalil, A. Din & R. M. Aadil, 2022. An inclusive overview of advanced thermal and nonthermal extraction techniques for bioactive compounds in food and food-related matrices. Food Reviews International, 38 (6): 1166-1196.