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Dergiler / Experimed / 2022 / Volume: 12 Issue: 3

Clues to the Harmful Effects of Aspartame on Liver Morphology and Function

Emine Rümeysa Hekimoğlu(BEZM-İ ÂLEM VAKIF ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, HİSTOLOJİ VE EMBRİYOLOJİ ANABİLİM DALI)0000-0003-4300-7213
Birsen Elibol(İSTANBUL MEDENİYET ÜNİVERSİTESİ)0000-0002-9462-0862
Ceyhun Toruntay(ISTANBUL TECHNICAL UNIVERSITY, FACULTY OF ARTS AND SCIENCES, DEPARTMENT OF MOLECULAR BIOLOGY AND GENETICS, DEPARTMENT OF MOLECULAR BIOLOGY AND GENETICS)0000-0002-4743-0257
Seda Kırmızıkan(BEZM-İ ÂLEM VAKIF ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, HİSTOLOJİ VE EMBRİYOLOJİ ANABİLİM DALI)0000-0002-5652-778X
Ozge Pasin(BEZM-İ ÂLEM VAKIF ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0001-6530-0942
Ufuk Sarıkaya(BEZM-İ ÂLEM VAKIF ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ FAKÜLTESİ)0000-0003-4573-5505
Damla Alkhalidi(BEZM-İ ÂLEM VAKIF ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0001-8553-659X
Mukaddes Eşrefoğlu(BEZM-İ ÂLEM VAKIF ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0003-3380-1480
Sayfa
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Yayın Tarihi
2022-12-31
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Conclusion: This study has concluded the prolonged use of high doses of aspartame to be able to cause damage to hepatocytes by stimulating oxidative stress, hepatocyte apoptosis, and necrosis.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: Aspartame is a widely used artificial sweetener that was approved by the United States Food and Drug Administration (FDA) in 1996 for use as a general sweetener in all foods.
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Temel Bulgular & Veri:The knowledge obtained from previous studies has been insufficient and contradictory, thus the aim of this study was to demonstrate the harmful side effects of daily and high doses of aspartame on the rat livers.
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Sonuç & Katkı:Conclusion: This study has concluded the prolonged use of high doses of aspartame to be able to cause damage to hepatocytes by stimulating oxidative stress, hepatocyte apoptosis, and necrosis.
🔬Tür:Clinical Research
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:aspartame · liver · light microscope

Abstract

Objective: Aspartame is a widely used artificial sweetener that was approved by the United States Food and Drug Administration (FDA) in 1996 for use as a general sweetener in all foods. Previous studies on aspartame had suggested it to be non-toxic. However, some studies have reported it to have carcinogenic, neurotoxic, apoptotic, and inflammatory effects. The knowledge obtained from previous studies has been insufficient and contradictory, thus the aim of this study was to demonstrate the harmful side effects of daily and high doses of aspartame on the rat livers. Materials and Methods: The study separated 18 Long Evans rats weighing between 250-300g into three groups: control, low dosage, and high dosage groups (n = 6 in each). 50 mg/kg of aspartame was given to the low dose group and 250 mg/kg to the high dose group every day for 10 weeks. At the end of the 10th week, all groups were euthanized and their livers and blood samples collected. Liver tissues were subjected to hematoxylin-eosin and Masson’s trichome staining, after which terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunohistochemistry was performed to check the serum alanine transaminase (ALT) and aspartate transaminase (AST) values. The enzyme-linked immunosorbent assay (ELISA) method was applied for analyzing superoxide dismutase (SOD) and malondialdehyde (MDA) levels. Results: Enlargement of the bile canaliculi and dilatation of sinusoids were observed in the group that was given high doses of aspartame. At the same time, the amount of TUNEL-positive cells was higher in the high dose group. AST, ALT, and MDA values were increased while SOD values were decreased in both the low and high aspartame dosage groups. Conclusion: This study has concluded the prolonged use of high doses of aspartame to be able to cause damage to hepatocytes by stimulating oxidative stress, hepatocyte apoptosis, and necrosis.

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Bu Makaleye Atıf Yap

Hekimoğlu, E. R., Elibol, B., Toruntay, C., Kırmızıkan, S., Pasin, O., Sarıkaya, U., Alkhalidi, D., Eşrefoğlu, M. (2022). Clues to the Harmful Effects of Aspartame on Liver Morphology and Function. Experimed, Volume: 12 Issue: 3. https://doi.org/10.26650/experimed.1192759

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