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Dergiler / Experimed / 2022 / Volume: 12 Issue: 3

Increased Perforin- and IL-21-Expressing NK Cells in Patients with Early-Stage Chronic Lymphocytic Leukemia

Fatih Akboğa(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE, THE DEPARTMENT OF IMMUNOLOGY)0000-0002-0202-3244
Fehmi Hindilerden(UNIVERSITY OF HEALTH SCIENCES, İSTANBUL BAKIRKÖY DR. SADİ KONUK TRAINING RESEARCH CENTER, DEPARTMENT OF INTERNAL MEDICINE)0000-0002-6297-9555
Emine Gulturk(UNIVERSITY OF HEALTH SCIENCES, İSTANBUL BAKIRKÖY DR. SADİ KONUK TRAINING RESEARCH CENTER, DEPARTMENT OF INTERNAL MEDICINE)0000-0003-2836-6162
İpek Yönal Hindilerden(ISTANBUL UNIVERSITY, İSTANBUL FACULTY OF MEDICINE, DEPARTMENT OF INTERNAL MEDICINE)0000-0003-1353-2367
Abdullah Yılmaz(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE, THE DEPARTMENT OF IMMUNOLOGY)0000-0002-3003-9956
Gunnur Deniz(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE, THE DEPARTMENT OF IMMUNOLOGY)0000-0002-0721-6213
Metin Yusuf Gelmez(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE, THE DEPARTMENT OF IMMUNOLOGY)0000-0002-5279-0855
Sayfa
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Yayın Tarihi
2022-12-31
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Conclusion: The findings suggest that in the early stage of CLL, increased total NK cell frequency, and elevated perforin and IL-21 levels in NK cells might have a protective impact against the progression of the disease for CLL patients.

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Amaç & Hipotez:Objective: CD5+CD19+ cells have a low proliferation capacity and elevated expression of anti-apoptotic protein BCL-2, mostly in the G0/G1 cell phase and accumulate in the pathogenesis of chronic lymphocytic leukemia(CLL).
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Temel Bulgular & Veri:Materials and Methods: In this study, peripheral blood mononuclear cells were isolated from peripheral blood samples of CLL patients (n=29) and healthy controls (n=16) by density gradient centrifugation method.The frequency of the total NK cells and the intracellular levels of perforin, granzyme, interferon (IFN)-γ, interleukin (IL)-21, IL-4 and IL-17 in NK cells were investigated.
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Sonuç & Katkı:Conclusion: The findings suggest that in the early stage of CLL, increased total NK cell frequency, and elevated perforin and IL-21 levels in NK cells might have a protective impact against the progression of the disease for CLL patients.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Chronic Lymphocytic Leukemia · CLL · Natural Killer Cells

Abstract

Objective: CD5+CD19+ cells have a low proliferation capacity and elevated expression of anti-apoptotic protein BCL-2, mostly in the G0/G1 cell phase and accumulate in the pathogenesis of chronic lymphocytic leukemia(CLL). Natural killer (NK) cells have the ability to kill the intracellular pathogen-infected or cancer cells and secrete cytotoxic enzymes. This study evaluated the frequency, expression of cytotoxic enzymes, and intracellular cytokine levels of NK cells in CLL patients. Materials and Methods: In this study, peripheral blood mononuclear cells were isolated from peripheral blood samples of CLL patients (n=29) and healthy controls (n=16) by density gradient centrifugation method.The frequency of the total NK cells and the intracellular levels of perforin, granzyme, interferon (IFN)-γ, interleukin (IL)-21, IL-4 and IL-17 in NK cells were investigated. Results: Elevatedtotal NK cell frequency were found in the CLL patients compared to the healthy controls, and negatively correlated with CD5+CD19+ malign B cell frequency. Increased perforin expression was observed in patients’ total NK cells. Additionally, increased levels of IL-17 and IL-21 in total NK cell of CLL patients were obtained compared to healthy subjects. Conclusion: The findings suggest that in the early stage of CLL, increased total NK cell frequency, and elevated perforin and IL-21 levels in NK cells might have a protective impact against the progression of the disease for CLL patients.

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Bu Makaleye Atıf Yap

Akboğa, F., Hindilerden, F., Gulturk, E., Hindilerden, İ. Y., Yılmaz, A., Deniz, G., Gelmez, M. Y. (2022). Increased Perforin- and IL-21-Expressing NK Cells in Patients with Early-Stage Chronic Lymphocytic Leukemia. Experimed, Volume: 12 Issue: 3. https://doi.org/10.26650/experimed.1191622

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