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Dergiler / Experimed / 2025 / Volume: 15 Issue: 3

Targeting Ras Signaling via Carbonic Anhydrase IX Inhibition: The Differential Impact of Acetazolamide on Clear Cell Renal Carcinoma and Melanoma Cells

Emine Terzi(ANKARA YILDIRIM BEYAZIT ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0001-9106-3848
Beyza Ecem Oz Bedir(ANKARA YILDIRIM BEYAZIT ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-0596-834X
Ahmet Çarhan(ANKARA YILDIRIM BEYAZIT ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0003-1584-0072
Özen Özensoy Güler(ANKARA YILDIRIM BEYAZIT ÜNİVERSİTESİ, TIP FAKÜLTESİ, TIP PR.)0000-0003-0389-9624
Sayfa
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Yayın Tarihi
2025-12-31
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These findings provide evidence supporting the role of AAZ in regulating Ras-MAPK signaling in melanoma.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: To investigate the effect of carbonic anhydrase IX (CA-IX) inhibition by acetazolamide (AAZ) on Ras protein expression in clear cell renal carcinoma and melanoma cell lines.
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Temel Bulgular & Veri:Materials and Methods: CAKI-2 (clear cell renal carcinoma) and A375 (melanoma) cell lines were cultured and treated with AAZ, rapamycin (RAPA), or vemurafenib (VMF).
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Sonuç & Katkı:These findings provide evidence supporting the role of AAZ in regulating Ras-MAPK signaling in melanoma.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:CA-IX · Clear cell renal carcinoma · MAPK

Abstract

Objective: To investigate the effect of carbonic anhydrase IX (CA-IX) inhibition by acetazolamide (AAZ) on Ras protein expression in clear cell renal carcinoma and melanoma cell lines. Materials and Methods: CAKI-2 (clear cell renal carcinoma) and A375 (melanoma) cell lines were cultured and treated with AAZ, rapamycin (RAPA), or vemurafenib (VMF). Cytotoxicity was assessed using the WST-1 assay. CA-IX inhibition was confirmed by enzyme-linked immunosorbent assay (ELISA) and immunofluo rescence staining. Ras protein expression levels were determined by Western blot analysis. Results: In CAKI-2 cells, there was no significant change in Ras protein expression following AAZ or RAPA treatment (p>0.05). In A375 melanoma cells, AAZ treatment significantly reduced Ras protein levels compared with both the untreated control and VMF-treated groups (p<0.001). Conclusion: AAZ significantly reduced Ras protein expression in A375 melanoma cells. These findings provide evidence supporting the role of AAZ in regulating Ras-MAPK signaling in melanoma.

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Bu Makaleye Atıf Yap

Terzi, E., Bedir, B. E. O., Çarhan, A., Güler, Ö. Ö. (2025). Targeting Ras Signaling via Carbonic Anhydrase IX Inhibition: The Differential Impact of Acetazolamide on Clear Cell Renal Carcinoma and Melanoma Cells. Experimed, Volume: 15 Issue: 3. https://doi.org/10.26650/experimed.1703562

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