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Dergiler / Experimed / 2026 / Volume: 16 Issue: 1

Cistus creticus L. Extracts: Investigation of Antioxidant and Enzyme Inhibitory Potential Through In vitro Analysis

Aksun Talu(NEAR EAST UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY (PHARMACY))0009-0005-9813-9063
Ali Şen(MARMARA UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PROFESSIONAL PHARMACEUTICAL SCIENCES, DEPARTMENT OF PHARMACOGNOSY)0000-0002-2144-5741
Duygu Mısırlı(UNIVERSITY OF HEALTH SCIENCES, FACULTY OF PHARMACY, DEPARTMENT OF BASIC PHARMACEUTICAL SCIENCES, DEPARTMENT OF BIOCHEMISTRY (PHARMACY))0000-0002-3581-4368
Mahfuz Elmastaş(UNIVERSITY OF HEALTH SCIENCES, FACULTY OF PHARMACY, DEPARTMENT OF BASIC PHARMACEUTICAL SCIENCES, DEPARTMENT OF BIOCHEMISTRY (PHARMACY))0000-0002-7149-7427
İsmail Şenkardeş(MARMARA UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF BASIC PHARMACEUTICAL SCIENCES, DEPARTMENT OF PHARMACEUTICAL MICROBIOLOGY, PHARMACEUTICAL BOTANY)0000-0002-2656-0319
Sayfa
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Yayın Tarihi
2026-08-27
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These results indicated that CCEA, CCW, and CCE exhibited strong antioxidant, good anti-inflammatory and anti-hyaluronidase activities, respectively.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: The aim of this study was to comparatively analyze the in vitro free radical scavenging and enzyme inhibitory activities along with the phytochemical content of Cistus creticus L.
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Temel Bulgular & Veri:Materials and Methods: The antioxidant and anti-enzyme effects were investigated using 2,2-diphenylpicrylhydrazyl (DPPH)/2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radicals and 5-lipoxygenase (LOX)/ hyaluronidase inhibition methods.
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Sonuç & Katkı:These results indicated that CCEA, CCW, and CCE exhibited strong antioxidant, good anti-inflammatory and anti-hyaluronidase activities, respectively.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Cistus creticus · 5-lipoxygenase inhibitory activity · antioxidant activity

Abstract

Objective: The aim of this study was to comparatively analyze the in vitro free radical scavenging and enzyme inhibitory activities along with the phytochemical content of Cistus creticus L. Materials and Methods: The antioxidant and anti-enzyme effects were investigated using 2,2-diphenylpicrylhydrazyl (DPPH)/2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radicals and 5-lipoxygenase (LOX)/ hyaluronidase inhibition methods. The total phenolic, flavonoid, and triterpenoid contents were tested using standard procedures. Phytochemical analysis was performed using high performance liquid chromatography (HPLC). Results: C. creticus ethyl acetate extract (CCEA) with 12.24 and 73.89 µg/mL IC50 values showed the strongest activity against ABTS and DPPH radicals, respectively. CCW (C. creticus water extract) with 66.16 µg/mL IC50 value showed the best anti-5-LOX activity. CCE (C. creticus ethanol extract) with 90.29 µg/mL IC50 value exhibited the strongest inhibitory effect against the hyaluronidase enzyme. The highest contents of total phenolic and flavonoid were detected in CCEA, whereas the highest total triterpenoid content was calculated in C. creticus hexane extract (CCH). In HPLC analysis, gallic acid and rutin were determined in CCE and CCEA; gallic acid and vanillic acid in CCW. Conclusion: This is the first study on the anti-hyaluronidase and anti-LOX effects of C. creticus . These results indicated that CCEA, CCW, and CCE exhibited strong antioxidant, good anti-inflammatory and anti-hyaluronidase activities, respectively.

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Bu Makaleye Atıf Yap

Talu, A., Şen, A., Mısırlı, D., Elmastaş, M., Şenkardeş, İ. (2026). Cistus creticus L. Extracts: Investigation of Antioxidant and Enzyme Inhibitory Potential Through In vitro Analysis. Experimed, Volume: 16 Issue: 1. https://doi.org/10.26650/experimed.1835502

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