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Dergiler / Trakya University Journal of Natural Sciences / 2025 / Volume: 26 Issue: 2

Chemical composition of endemic Hypericum bilgehan-bilgilii Basköse & Savran essential oil and its α-glucosidase antidiabetic, anti-inflammatory, cytotoxic and antioxidant potentials

Hüseyin Servi(YENİ YÜZYIL ÜNİVERSİTESİ)0000-0002-4683-855X
Timur Hakan Barak(Acıbadem Mehmet Ali Aydınlar Üniversitesi)0000-0002-7434-3175
Ali Şen(MARMARA ÜNİVERSİTESİ)0000-0002-2144-5741
Simge Ertekin(YENİ YÜZYIL ÜNİVERSİTESİ)0000-0002-7976-7057
Hüseyin Türker(NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ)0000-0001-7309-9484
Bengü Türkyılmaz Ünal(NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ)0000-0003-4003-5200
Cemil İşlek(NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ)0000-0002-6690-2846
Sayfa
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Yayın Tarihi
2025-10-15
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

İlginç bir şekilde, majör uçucular: 1-(2,4,5-trimetoksifenil)bütan-1-on (%27,7) ve 3-Metil-1-(2,4,6-trihidroksifenil)bütan-1-on (%11,2) Hypericum uçucu yağlarında ilk kez tespit edilmiştir.

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Amaç & Hipotez:Hypericum cinsi, tıbbi ve kozmetik faydaları olan fitokimyasallar bakımından zengin olması nedeniyle Türkiye florası için önemli bir cinstir.
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Temel Bulgular & Veri:Kimyasal bileşim, polar olmayan kolon kullanılarak GC-MS ile analiz edilmiştir. α-glukozidaz ve 5-lipoksijenaz inhibitör, sitotoksik ve DPPH radikal süpürücü aktiviteleri araştırılmıştır.
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Sonuç & Katkı:İlginç bir şekilde, majör uçucular: 1-(2,4,5-trimetoksifenil)bütan-1-on (%27,7) ve 3-Metil-1-(2,4,6-trihidroksifenil)bütan-1-on (%11,2) Hypericum uçucu yağlarında ilk kez tespit edilmiştir.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Aromatherapy · Gas Chromatography-Mass Spectrometry · GC-FID

Özet

Hypericum cinsi, tıbbi ve kozmetik faydaları olan fitokimyasallar bakımından zengin olması nedeniyle Türkiye florası için önemli bir cinstir. Hypericum bilgehan-bilgilii Basköse & Savran'ın (HEO)’nin uçucu yağ bileşimi ve biyolojik aktiviteleri bu çalışmada ilk kez analiz edilmiştir. Hypericum bilgehan-bilgilii tüm kısımlarının uçucu yağı, Clevenger tipi aparatla hidrodistilasyon yoluyla elde edilmiştir. Kimyasal bileşim, polar olmayan kolon kullanılarak GC-MS ile analiz edilmiştir. α-glukozidaz ve 5-lipoksijenaz inhibitör, sitotoksik ve DPPH radikal süpürücü aktiviteleri araştırılmıştır. Kırk sekiz bileşen tanımlanmış ve tüm bileşenlerin %97,3'ünü temsil etmiştir. İlginç bir şekilde, majör uçucular: 1-(2,4,5-trimetoksifenil)bütan-1-on (%27,7) ve 3-Metil-1-(2,4,6-trihidroksifenil)bütan-1-on (%11,2) Hypericum uçucu yağlarında ilk kez tespit edilmiştir. Yağ, 39 µg/mL'lik bir IC50 değeriyle 5-lipoksijenaz enzimine karşı önemli bir aktivite göstermiştir. HEO'nun sitotoksisite potansiyeli, farklı konsantrasyonlarda, dört hücre hattına karşı araştırılmış ve IC50 değerleri hafif sitotoksisitenin varlığını göstermiştir. Bu sonuçlar, H. bilgehan-bilgilii uçucu yağının biyoaktif bileşenler ve anti-inflamatuar ajanlar için değerli bir kaynak olarak kabul edilebileceğini göstermiştir.

Abstract

Hypericum L. is a significantly important genus for flora of Türkiye due to its richness in phytochemicals possessing medicinal and cosmetic benefits. The essential oil composition and biological activities of Hypericum bilgehan-bilgilii Basköse & Savran (HEO) were analysed for the first time in the present study. The volatile oil of the whole parts of H. bilgehan-bilgilii was obtained by hydrodistillation with Clevenger-type apparatus. The chemical composition was analyzed by GC-MS using non-polar column. α-glucosidase and 5-lipoxygenase inhibitory, cytotoxic, and DPPH radical scavenging activities were investigated. Forty-eight components were identified and represented 97.3% of the whole constituents. Interestingly, the major volatiles: 1-(2,4,5-trimethoxyphenyl)butan-1-one (27.7%) and 3-Methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one (11.2%) were detected for the first time in Hypericum essential oils. The oil exhibited a significant activity against the 5-lipoxygenase enzyme with an IC50 value of 39 µg/mL. Cytotoxicity potential of HEO was investigated, at different concentrations, towards four cell lines and, IC50 values underlies mild cytotoxicity. These results indicated that H. bilgehan-bilgilii essential oil may be considered as a valuable source for bioactive ingredients and anti-inflammatory agents.

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Bu Makaleye Atıf Yap

Servi, H., Barak, T. H., Şen, A., Ertekin, S., Türker, H., Ünal, B. T., İşlek, C. (2025). Chemical composition of endemic Hypericum bilgehan-bilgilii Basköse & Savran essential oil and its α-glucosidase antidiabetic, anti-inflammatory, cytotoxic and antioxidant potentials. Trakya University Journal of Natural Sciences, Volume: 26 Issue: 2. https://ajindex.com/articles/1111936

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