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Dergiler / ODÜ Tıp Dergisi / 2026 / Cilt: 13 Sayı: 2

Deneysel Olarak İntraabdominal Sepsis Oluşturulan Ratlarda Amiodaronun Böbrekler Üzerindeki Koruyucu Etkilerinin Değerlendirilmesi

Evaluation of the Protective Effects of Amiodarone on the Kidneys in Rats with Experimentally Induced Intra-Abdominal Sepsis

Onur Yalçın(Ordu Üniversitesi)0000-0001-9377-1039
Akgün Oral(SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, İZMİR TIP FAKÜLTESİ)0000-0002-5250-7335
Zekai Halıcı(ATATÜRK ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0001-6854-6059
Yasin Bayır(ATATÜRK ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0003-3562-6727
Murat Yiğiter(ATATÜRK ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-4310-0752
Ahmet Salman(ATATÜRK ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-7435-6865
Sayfa
56–67
Yayın Tarihi
2026-10-01
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular: CLP ile indüklenen sepsis, kreatinin, üre, malondialdehit, IL-1β mRNA ve TNF-α mRNA düzeylerinde anlamlı bir artışa neden olmuştur; buna paralel olarak, süperoksit dismutaz ve glutatyon düzeylerinde anlamlı bir azalma gözlenmiştir.

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Amaç & Hipotez:Amaç: Bu çalışmada, sıçanlarda intraabdominal sepsis deney modelinde amiodaronun böbrek koruyucu potansiyeli araştırılmıştır.
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Temel Bulgular & Veri:Gereç ve Yöntemler: Toplam 100 erkek Wistar sıçanı beş gruba ayrılmıştır: sham cerrahi uygulanan bir kontrol grubu; sham cerrahi uygulanan ve 50 mg/kg dozunda amiodaron verilen bir kontrol grubu; çekal ligasyon ve perforasyon (CLP) uygulanan bir grup; CLP uygulanan ve 25 mg/kg dozunda amiodaron verilen bir grup; ve CLP uygulanan ve 50 mg/kg dozunda amiodaron verilen bir grup.
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Sonuç & Katkı:Bulgular: CLP ile indüklenen sepsis, kreatinin, üre, malondialdehit, IL-1β mRNA ve TNF-α mRNA düzeylerinde anlamlı bir artışa neden olmuştur; buna paralel olarak, süperoksit dismutaz ve glutatyon düzeylerinde anlamlı bir azalma gözlenmiştir.
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:sepsis · akut böbrek yetmezliği · amiodaron

Özet

Amaç: Bu çalışmada, sıçanlarda intraabdominal sepsis deney modelinde amiodaronun böbrek koruyucu potansiyeli araştırılmıştır. Gereç ve Yöntemler: Toplam 100 erkek Wistar sıçanı beş gruba ayrılmıştır: sham cerrahi uygulanan bir kontrol grubu; sham cerrahi uygulanan ve 50 mg/kg dozunda amiodaron verilen bir kontrol grubu; çekal ligasyon ve perforasyon (CLP) uygulanan bir grup; CLP uygulanan ve 25 mg/kg dozunda amiodaron verilen bir grup; ve CLP uygulanan ve 50 mg/kg dozunda amiodaron verilen bir grup. Sepsis modelini oluşturmak için CLP tekniği kullanılmış ve amiodaron, ameliyat sırasında bir tüp aracılığıyla ağız yoluyla verilmiştir. Bulgular: CLP ile indüklenen sepsis, kreatinin, üre, malondialdehit, IL-1β mRNA ve TNF-α mRNA düzeylerinde anlamlı bir artışa neden olmuştur; buna paralel olarak, süperoksit dismutaz ve glutatyon düzeylerinde anlamlı bir azalma gözlenmiştir. Amiodaron, bu değişiklikleri doza bağlı bir şekilde tersine çevirdi. 50 mg/kg'lık doz, tüm biyokimyasal, moleküler ve histolojik parametrelerde en kapsamlı iyileşmeye yol açtı. Sonuç: Sonuçlar, amiodaronun sepsisle ilişkili akut böbrek hasarını hafifletebileceğini göstermektedir. Bu etki muhtemelen ilacın antioksidan ve antienflamatuar özelliklerinin birleşimi yoluyla ortaya çıkmaktadır.

Abstract

Objective: This study examined the renoprotective potential of amiodarone in an experimental rat model of intra-abdominal sepsis. Materials and Methods: A total of 100 male Wistar rats were distributed into five groups: sham-operated controls, sham controls receiving amiodarone 50 mg/kg, cecal ligation and puncture (CLP), CLP with amiodarone 25 mg/kg, and CLP with amiodarone 50 mg/kg. The CLP technique was employed to establish the sepsis model, and amiodarone was delivered orally via gavage at the time of surgery. Results: Sepsis induction via CLP caused significant increases in creatinine, urea, malondialdehyde, IL-1β mRNA, and TNF-α mRNA, accompanied by significant decreases in superoxide dismutase and glutathione. Amiodarone reversed these alterations in a dose-dependent manner, with the 50 mg/kg dose producing the most comprehensive improvements across all biochemical, molecular, and histological measures. Conclusion: The results suggest that amiodarone may mitigate sepsis-associated acute kidney injury, an effect likely mediated through its combined antioxidant and anti-inflammatory properties.

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Bu Makaleye Atıf Yap

Yalçın, O., Oral, A., Halıcı, Z., Bayır, Y., Yiğiter, M., Salman, A. (2026). Deneysel Olarak İntraabdominal Sepsis Oluşturulan Ratlarda Amiodaronun Böbrekler Üzerindeki Koruyucu Etkilerinin Değerlendirilmesi. ODÜ Tıp Dergisi, Cilt: 13 Sayı: 2, 56–67. https://doi.org/10.56941/odutip.2007091

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