Dergiler / Bozok Journal of Science / 2023 / Cilt: 1 Sayı: 2

Exploring the Bacterial Diversity of the Armutlu Geothermal Spring (Türkiye) through a Metagenomic Sequenced Based Approach

Sayfa
57–64
Yayın Tarihi
2023-11-30
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

As a conclusion, the microbiota of the Armutlu geothermal spring was open to investigate the novel species and hydrolytic biocatalysts.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:The geothermal or hot springs are home to a diverse microbial population.
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Temel Bulgular & Veri:During the period of sampling, the water temperature and pH at the surface were 74.9 °C and 7.1, respectively.
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Sonuç & Katkı:As a conclusion, the microbiota of the Armutlu geothermal spring was open to investigate the novel species and hydrolytic biocatalysts.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Bacterial community · Armutlu geothermal spring · Thermophile

Abstract

The geothermal or hot springs are home to a diverse microbial population. Identification of the thermophilic microbial world of these niches can direct us to the exploration of novel economically valuable biocatalysts, antibiotics, and a variety of other biomolecules. Due to the tectonic features within its borders, Türkiye has many thermal springs in different regions. In this study, a thermal site, the Armutlu geothermal spring in the eastern Marmara region, was investigated to identify the bacterial taxonomic composition and diversity through a metagenomic sequence-based approach which is the first study to the best of our knowledge. During the period of sampling, the water temperature and pH at the surface were 74.9 °C and 7.1, respectively. According to 16S rRNA sequencing results, Bacteria (98.36%) are the dominant taxa compared to Archaea (1.64%). Based on the diversity findings, the bacterial community is primarily dominated by Proteobacteria (42.49%), which, together with Chloroflexi, Acidobacteria, Actinobacteria, Firmicutes, Nitrospirae, and Bacteroidetes, constitute ~87% of the overall bacterial population. Furthermore, the microbial community was also investigated for the activity of hydrolase enzymes such as protease, amylase, and amylopullulanase through a culture-dependent approach for possible future applications. As a conclusion, the microbiota of the Armutlu geothermal spring was open to investigate the novel species and hydrolytic biocatalysts.

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Bu Makaleye Atıf Yap

CEBECİ, A., ÖZTUĞ, M., MUMCU, H., KARAGÜLER, N. G. (2023). Exploring the Bacterial Diversity of the Armutlu Geothermal Spring (Türkiye) through a Metagenomic Sequenced Based Approach. Bozok Journal of Science, Cilt: 1 Sayı: 2, 57–64. https://ajindex.com/articles/1020365

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Kaynakça (2)

  1. P. H. Rampelotto, “Extremophiles and Extreme Environments,” Life, vol. 3, no. 3, pp. 482–485, Aug. 2013, doi:10.3390/life3030482. L. Sawle and K. Ghosh, “How Do Thermophilic Proteins and Proteomes Withstand High Temperature?,” Biophys. J., vol. 101, no. 1, pp. 217–227, Jul. 2011, doi:10.1016/j.bpj.2011.05.059. M. E. DeCastro, E. Rodriguez-Belmonte, and M. I. Gonzalez-Siso, “Metagenomics of Thermophiles with a Focus on Discovery of Novel Thermozymes,” Front. in Microbiol., vol. 7, 1521, Sept. 2016, doi:10.3389/fmicb.2016.01521. J. Atalah, P. Cáceres-Moreno, G. Espina, and J. M. Blamey, “Thermophiles and the Applications of Their Enzymes as New Biocatalysts,” Bioresour. Technol., vol. 280, pp. 478–488, May. 2019, doi:10.1016/j.biortech.2019.02.008. U. Serpen and R. DiPippo, “Turkey-A Geothermal Success Story: A Retrospective and Prospective Assessment,” Geothermics, vol. 101, 102370, May. 2022, doi:10.1016/j.geothermics.2022.102370. Maden Tetkik ve Arama Genel Müdürlüğü (MTA), “Türkiye Jeotermal Enerji Potansiyeli ve Arama Çalışmaları,” https://www.mta.gov.tr/v3.0/arastirmalar/jeotermal-enerji-arastirmalari. [Access Date: 12-April-2023].
  2. A. Adıgüzel, K. İnan, F. Şahin, T. Arasoğlu, M. Güllüce, A. O. Beldüz, and Z. Barış, “Molecular Diversity of Thermophilic Bacteria Isolated from Pasinler Hot Spring,” Turk. J. Biol., vol. 35, no. 3, pp. 267–274, Jan. 2011, doi:10.3906/biy-0908-7. E. Celikoglu and M. Yilmaz Cankilic, “Prokaryotic Diversity of Hot Springs in Balikesir and Kütahya, Turkey,” Appl. Ecol. Environ. Res., vol. 14, no. 4, pp. 429–440, Sep. 2016, doi:10.15666/aeer/1404_429440. K. Inan, A. Ozer, H. I. Güler, A. O. Belduz, and S. Canakci, “Brevibacillus gelatini sp. nov., Isolated from a Hot Spring,” Int. J. Syst. Evol., vol. 66, no. 2, pp. 712–718, Feb. 2016, doi:10.1099/ijsem.0.000780. M. O. Baltaci, H. Ay, S. Akbulut, G. Adiguzel, S. Albayrak, M. A. Omeroglu, H. Ozkan, M. Taskın, and A. Adiguzel, “Bacillus pasinlerensis sp. nov., a Thermophilic Bacterium Isolated from a Hot Spring in Turkey,” Int. J. Syst. Evol. Microbiol., vol. 70, no. 6, pp. 3865–3871, Jun. 2020, doi:10.1099/ijsem.0.004246. S. Çalışkan-Özdemir, S. Önal, and A. Uzel, “Partial Purification and Characterization of a Thermostable Phytase Produced by Thermotolerant Aspergillus tubingensis TEM 37 Isolated from Hot Spring Soil in Gediz Geothermal Field, Turkey,” Geomicrobiol. J., vol. 38, no. 10, pp. 895–904, Sept. 2021, doi:10.1080/01490451.2021.1977432. F. Matpan Bekler, K. Güven, and R