Patojen bir bakteri olan Aeromonas hydrophilia’ nın probiyotik kullanılarak eliminasyonu

Elimination of pathogenic bacterium, Aeromonas hydrophila by the use of probiotics

Abstract

The present investigation was carried out to study the elimination of pathogenic, chloroamphinicol resistant Aeromonas hydrophila (A. hydrophilaChr) by the use of single probiotics; Probiotic 1 (Lactobacillus sporogenes), Probiotic 2 (Saccharomyces boulardii) or mixture of probiotics; Probiotic 3 (Nitromonas spp., Rhodococcus spp., Bacilus megaterium, Lecheni formis, Desulphovibrio sulphuricum, Psuedomonas spp., Chromatium spp., Chlorobium spp., Thiobacillus spp., Thioxidants spp., Thiobacilus ferroxidant, Methylomonas methyanica, Glucon acetobactor, Azospirillum spp., Trichoderma spp., Shizophyllum commune and Sclertium gluconicum); in vitro as well as in vivo. For this purpose probiotics 1, 2 and 3 were tested against the pathogenic Aeromonas hydrophilachr. In vitro experiment revealed that the zones of inhibition of probiotic 1 were highest than probiotic 3 followed by probiotic 2 with values of 19.67 ± 0.67, 19.33 ± 0.33 and 17.00 ±0.58 mm, respectively. In vivo experiment also showed that the elimination of pathogenic A. hydrophilachr from 1.54 x 1011 CFU/mL to 1.90 x 101, 2.30 x 101 and 5.33 x 101 CFU/mL lasted four weeks by probiotic 1, probiotic 3 and probiotic 2, respecticvely. In conclusion, the viable counts of pathogenic bacterium were the highest in the fish inoculated only with the pathogenic bacteria 6.07x1012 cells/mL in four weeks. Probiotic cultures used had significantly reduced the viable count of A. hydrophila in fish. The numbers of viable counts was the lowest in catfishes treated with probiotic 1 followed by probiotic 3 and probiotic 2 over a period of four weeks.

Özet

Bu çalışmada, Chloramhinicol’e dirençli Aeromonas hydrophilia’nın tekil probiyotikler; Probiyotik 1 (Lactobacillus sporogenes), Probiotic 2 (Saccharomyces boulardii) veya karışım Probiyotik 3 (Nitromonas spp., Rhodococcus spp., Bacilus megaterium, Lecheniformis, Desulphovibrio sulphuricum,Psuedomonas spp.,Chromatium spp., Chlorobium spp.,Thiobacillus spp., Thioxidants spp., Thiobacilus ferroxidant,Methylomonasme thyanica, Glucon acetobactor, Azospirillum spp., Trichoderma spp., Shizophyllum commune ve Sclertium gluconicum); kullanılarak eliminasyonu in vitro ve in vivo olarak incelenmiştir. Bu amaçla, probiyotik 1, 2 ve 3 patojenik Aeromonas hydrophiliachr üzerinde test edilmiştir. In vitro deneyler sonunda probiyotik 1’in inhibisyon zonu en yüksek olarak tespit edildi, onu probiyotik 3 ve 2 takip etti, elde edilen değerler sırasıyla 19.67 ± 0.67, 19.33 ± 0.33 ve 17.00 ±0.58 mm oldu. In vivo deneylerde ise A. hydrophilia’nın 1.54 x 1011CFU/mL değerinden probiyotik 1, 2 ve 3 için sırasıyla 1.90 x 101, 2.30 x 101 and 5.33 x 101 CFU/mL değerlerine indirilmesi dört hafta sürdü. Sonuç olarak, patojenik bakteriler dört hafta sonunda en yoğun 6.07x1012 Hücre/mL olarak sadece bakteri inoküle edilen balıklarda görüldü. Kullanılan probiyotikler balıklarda A. hydrophila canlı sayısında önemli azalmaya neden olmuştur. Dört haftalık denemenin sonunda canlı sayıları probiyotik 1 kullanılan kedi balıklarında en düşük tespit edildi, bunu probiyotik 3 ve probiyotik 2 takip etti.