SavedAdd your journal

Journals / Adli Bilimler ve Suç Araştırmaları / 2020 / Cilt: 2 Sayı: 1

Analytical Method Validation in Forensic Assay

Adli Analizde Analitik Yöntem Doğrulaması

Burcu DOĞAN TOPAL
İrem KAYA
Sibel A. ÖZKAN
Pages
9–24
Publication Date
2020-09-11
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Analytical method validation should include several performance factors such as: Selectivity, specificity, accuracy, precision, linearity, range, limit of detection (LOD), limit of quantification (LOQ), stability, ruggedness, and robustness etc.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Reliable analytical data is an essential requisite for accurate interpretation of toxicological findings in the forensic science.
📊
Key Findings & Data:If the analytical data is not reliable, it may lead to wrong legal processes for the defendant or the wrong treatment of the patient.
💡
Conclusion & Impact:Analytical method validation should include several performance factors such as: Selectivity, specificity, accuracy, precision, linearity, range, limit of detection (LOD), limit of quantification (LOQ), stability, ruggedness, and robustness etc.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Analitik Yöntemler · Adli Bilimler · Suç Analizi

Abstract

Reliable analytical data is an essential requisite for accurate interpretation of toxicological findings in the forensic science. If the analytical data is not reliable, it may lead to wrong legal processes for the defendant or the wrong treatment of the patient. Therefore, analytical methods to be used in forensic science require careful method development and validation. Method validation is the most important and key element in establishing reference methods and evaluating the ability of a laboratory to produce reliable analytical data. Newly developed or routine used analytical methods must validate according to the standard guidelines. Analytical method validation should include several performance factors such as: Selectivity, specificity, accuracy, precision, linearity, range, limit of detection (LOD), limit of quantification (LOQ), stability, ruggedness, and robustness etc.

Özet

Adli bilimlerde toksikolojik bulguların doğru yorumlanması için güvenilir analitik verilere ihtiyaç vardır. Analitik veri güvenilir değilse, bu, sanık için yanlış hukuki süreçlere veya hastanın yanlış tedavisine yol açabilir. Bu nedenle, adli bilimlerde kullanılacak analitik yöntemlerin dikkatli bir şekilde yöntem geliştirilmesi ve doğrulanması gerekmektedir. Yöntem doğrulaması, referans yöntemlerinin oluşturulmasında ve bir laboratuvarın güvenilir analitik veri üretebilme yeteneğinin değerlendirilmesinde en önemli ve anahtar unsurdur. Yeni geliştirilmiş veya rutin olarak kullanılan analitik yöntemler, standart rehberlere göre doğrulanmalıdır. Analitik yöntem doğrulaması, seçicilik, özgüllük, doğruluk, hassasiyet, doğrusallık, aralık, tespit sınırı (LOD), nicelleme sınırı (LOQ), stabilite, sağlamlık ve robustluk gibi birkaç performans faktörünü içermelidir.

🔖

Cite This Article

TOPAL, B. D., KAYA, İ., ÖZKAN, S. A. (2020). Analytical Method Validation in Forensic Assay. Adli Bilimler ve Suç Araştırmaları, Cilt: 2 Sayı: 1, 9–24. https://ajindex.com/articles/976167

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (7)

  1. Barnett, K.L., Harrington, B. ve Graul, T.W. (2013). Validation of liquid chromatographic methods. Hollanda: Elsevier. Barron, L., Gilchrist, E. (2014). Ion chromatography-mass spectrometry: A review of recent technologies and applications ın forensic and environmental explosives analysis. Analytica Chimica Acta. C. 806, ss. 27-54.
  2. Blessy, M. R. D. P., Patel, R. D., Prajapati, P. N. ve Agrawal, Y. K. (2014). Development of forced degradation and stability ındicating studies of drugs-a review. Journal of Pharmaceutical Analysis. C. 4, S. 3, ss. 159-165.
  3. Burleson, G. L., Gonzalez, B., Simons, K. ve Jorn, C. C. (2009). Forensic analysis of a single particle of partially burnt gunpowder by solid phase micro-extraction-gas chromatography-nitrogen phosphorus detector. Journal of Chromatography A. C. 1216, S. 22, ss. 4679-4683.
  4. Chophi, R., Sharma, S., Sharma, S. ve Singh, R. (2019). Trends in the forensic analysis of cosmetic evidence. Forensic Chemistry. C. 14. Dogan-Topal, B., Li, W., Schinkel, A. H., Beijnen, J. H. ve Sparidans, R. W. (2019). Quantification of FGFR4 inhibitor BLU-554 in mouse plasma and tissue homogenates using liquid chromatography-tandem mass spectrometry. Journal of Chromatography B. C. 1110, ss. 116-123. Emniyet Genel Müdürlüğü Kriminal Daire Başkanlığı. (2019). Kimya. https://www.egm. gov.tr/kriminal/kimyauzmanlik. (E.T.: 16 Aralık 2019).
  5. Encylcopedia Britannica. (2019). Crime laboratory. https://www.britannica.com/ science/crime-laboratory#ref310184 ve https://www.britannica.com/science/crimelaboratory# ref310184. (E.T.: 16 Aralık 2019).
  6. Ermer, J., John, H. ve Miller, M. (2005). Method validation in pharmaceutical analysis. Weinheim. European Medicines Agency. (2003). Ich topic q 1 a (r2) stability testing of new drug substances and products step, ıch-stability test. New drug subst. Prod. 1–20. doi:10.1136/bmj.333.7574.873-a. Gallidabino, M. D., Irlam, R. C., Salt, M. C., O’Donnell, M., Beardah, M. S. ve Barron, L. P. (2019). Targeted and non-targeted forensic profiling of black powder substitutes and gunshot residue using gradient ıon chromatography-high resolution mass spectrometry (ıc-hrms). Analytica Chimica Acta. C. 1072, ss. 1-14. General Chapters: Validation of compendial methods, (n.d.). González, O., Alonso, R. M. (2020). Validation of bioanalytical chromatographic methods for the quantification of drugs in biological fluids. In Handbook of Analytical Separations. C. 7, ss. 115-134.
  7. Grapp, M., Kaufmann, C.,Streit, F. ve Binder,