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Journals / Cukurova Medical Journal / 2023 / Cilt: 48 Sayı: 3

The expression of TRPV6 and PMCA1 in the mid-secretory endometrium of infertile patients with unexplained infertility and endometriosis

Açıklanamayan infertilite ve endometriozisli infertil hastaların mid-sekretuar endometriyumunda TRPV6 ve PMCA1’in ekspresyonu

Tuba OZCAN METİN
Nafiye YILMAZ
Banu YILMAZ
Şakir Necat YILMAZ
Mehmet Emin ERDAL
İlay BURAN
Gülhan TEMEL
Adem DOĞANER
Pages
815–824
Publication Date
2023-09-30
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further studies are needed to elucidate the functional roles of these channels and to develop new strategies for pharmaceutical intervention in the treatment of infertility.

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: The goal of this study was to evaluate the expression of transient receptor potential vanilloid 6 (TRPV6) and plasma membrane Ca2+ ATPase 1 (PMCA1) in the endometrium of infertile women with unexplained infertility (UI) and endometriosis (E), and to compare the findings to healthy fertile women.
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Key Findings & Data:Materials and Methods: Endometrial expression of TRPV6 and PMCA1 proteins was evaluated during the mid-secretory phase using the immunohistochemical method, and mRNA levels were measured by real-time PCR.
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Conclusion & Impact:Further studies are needed to elucidate the functional roles of these channels and to develop new strategies for pharmaceutical intervention in the treatment of infertility.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Kalsiyum metabolizması · endometriozis · TRPV6

Abstract

Purpose: The goal of this study was to evaluate the expression of transient receptor potential vanilloid 6 (TRPV6) and plasma membrane Ca2+ ATPase 1 (PMCA1) in the endometrium of infertile women with unexplained infertility (UI) and endometriosis (E), and to compare the findings to healthy fertile women. Materials and Methods: Endometrial expression of TRPV6 and PMCA1 proteins was evaluated during the mid-secretory phase using the immunohistochemical method, and mRNA levels were measured by real-time PCR. Calcium concentrations were determined using Inductively coupled plasma-mass spectrometry (ICP-MS). Results: TRPV6 and PMCA1 protein expression were significantly increased in both luminal and glandular epithelium of UI or E groups compared to the control group. TRPV6 mRNA expression levels were significantly lower in the UI group (0.525 ± 0.087) compared to the control group (0.809 ± 0.073). There was no significant difference in PMCA1 mRNA expression levels between the groups. Calcium concentration was significantly higher in women with UI (median (Q1-Q3): 50.57 [40.67-51.37], compared to the control group (median (Q1-Q3): 24.29 [20.11-25.55]). Conclusion: The present findings demonstrate altered expressions of TRPV6 and PMCA1 mRNA and protein, along with differences in calcium concentration among patients, especially those with the UI during the window of implantation. These differences might potentially be considered as one of the underlying causes of infertility. Further studies are needed to elucidate the functional roles of these channels and to develop new strategies for pharmaceutical intervention in the treatment of infertility.

Özet

Amaç: Bu çalışmanın amacı, açıklanamayan infertilite (Aİ) ve endometriozisi (E) olan infertil kadınların endometriyumunda Transient Reseptör Potansiyel Vanilloid 6 (TRPV6) ve Plazma Membran Ca2+ ATPaz 1 (PMCA1) ekspresyonunu değerlendirmek ve bulguları sağlıklı fertil kadınlarla karşılaştırmaktır. Gereç ve Yöntem: TRPV6 ve PMCA1 proteinlerinin endometriyal ekspresyonu mid-sekretuar faz sırasında, immünohistokimyasal yöntemle değerlendirilmiş ve mRNA seviyeleri real-time PCR ile ölçülmüştür. Kalsiyum konsantrasyonları İndüktif Eşleşmiş Plazma-Kütle Spektrometrisi (ICP-MS) kullanılarak belirlenmiştir. Bulgular: TRPV6 ve PMCA1 protein ekspresyonu kontrol grubu ile karşılaştırıldığında, Aİ ve E gruplarının hem luminal hem de glandular epitelinde anlamlı olarak artış saptanmıştır. TRPV6 mRNA ekspresyon seviyeleri Aİ grubunda (0.525 ± 0.087) kontrol grubuna (0.809 ± 0.073) kıyasla anlamlı derecede düşüktür. PMCA1 mRNA ekspresyon düzeylerinde gruplar arasında anlamlı bir fark yoktur. Kalsiyum konsantrasyonu Aİ’li kadınlarda (medyan (Q1-Q3): 50.57 [40.67-51.37], kontrol grubuna (medyan (Q1-Q3): 24.29 [20.11-25.55]) göre anlamlı derecede yüksek bulunmuştur. Sonuç: Mevcut bulgular, implantasyon penceresi sırasında özellikle Aİ’li hastalarda, kalsiyum konsantrasyonlarındaki farklılıkların yanı sıra, TRPV6 ve PMCA1 mRNA ve proteinlerinin değişen ekspresyonlarını göstermektedir. Bu farklılıklar, potansiyel olarak infertilitenin altında yatan nedenlerden biri olarak düşünülebilir. Bu kanalların fonksiyonel rollerini aydınlatmak ve infertilite tedavisinde farmasötik yaklaşımda yeni stratejiler geliştirmek için daha fazla çalışmaya ihtiyaç vardır.

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Cite This Article

METİN, T. O., YILMAZ, N., YILMAZ, B., YILMAZ, Ş. N., ERDAL, M. E., BURAN, İ., TEMEL, G., DOĞANER, A. (2023). The expression of TRPV6 and PMCA1 in the mid-secretory endometrium of infertile patients with unexplained infertility and endometriosis. Cukurova Medical Journal, Cilt: 48 Sayı: 3, 815–824. https://ajindex.com/articles/966035

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