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Journals / Journal of Research in Veterinary Medicine / 2023 / Cilt: 42 Sayı: 1

Characterization and Computational Investigation of Polymorphisms in the TIR Domain of the TLR4 Protein in Holsteins Raised in Turkey

Sertaç ATALAY
Pages
37–43
Publication Date
2023-07-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Project Hope results showed that mutant and wild-type amino acids have different properties and that this can disturb the TIR domain.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Increasing environmental temperatures due to climate change adversely affect livestock welfare and health.
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Key Findings & Data:Moreover, temperatures increase the distribution and survival of parasites and infectious agents.
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Conclusion & Impact:Project Hope results showed that mutant and wild-type amino acids have different properties and that this can disturb the TIR domain.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:TLR4 · TIR domain · polymorphism

Abstract

Increasing environmental temperatures due to climate change adversely affect livestock welfare and health. Moreover, temperatures increase the distribution and survival of parasites and infectious agents. Livestock diseases that cause significant economic losses are a worldwide concern. Toll-like receptor 4 (TLR4) is an ideal marker gene candidate, due to its critical role it plays in initiating the immune response against pathogens. In this study, the toll-interleukin-1 receptor (TIR) domain polymorphisms were investigated in the Holsteins raised in Turkey. by DNA sequencing. The effects of polymorphisms on the protein structure and function were evaluated by computational tools (I-Mutant Suite, Project Hope and PyMOL). The rs8193069 C>T polymorphism was detected in the TIR domain. The SNP causes Threonine to Isoleucine substitution at position 674 of the TLR4 protein which is the second amino acid of the TIR domain. The I-Mutant Suite predicted that Thr674Ile substitution could decrease protein stability (DDG= -0.40 Kcal/mol). Project Hope results showed that mutant and wild-type amino acids have different properties and that this can disturb the TIR domain. The Thr674Ile polymorphism observed in the TIR domain of TLR4 protein in Holsteins raised in Turkey might affect the function of the TLR4 by causing physicochemical changes.

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Cite This Article

ATALAY, S. (2023). Characterization and Computational Investigation of Polymorphisms in the TIR Domain of the TLR4 Protein in Holsteins Raised in Turkey. Journal of Research in Veterinary Medicine, Cilt: 42 Sayı: 1, 37–43. https://ajindex.com/articles/957531

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References (16)

  1. Pinto J, Bonacic C, Hamilton-West C, Romero J, Lubroth J. Climate change and animal diseases in South America. Rev Sci Tech. 2008;27(2):599-613
  2. Lacetera N. Impact of climate change on animal health and welfare. Anim Front. 2019;9(1):26-31
  3. Yatoo M, Kumar P, Dimri U, Sharma M. Effects of climate change on animal health and diseases. Int. J. Livest. Res. 2012;2(3):15-24
  4. Ruegg PL. Investigation of mastitis problems on farms. Vet. Clin. North Am. Food Anim. Pract. 2003;19(1):47-73
  5. Bagath M, Krishnan G, Devaraj C, et al. The impact of heat stress on the immune system in dairy cattle: A review. Res. Vet. Sci. 2019;126:94-102
  6. Bharati J, Dangi S, Chouhan V, et al. Expression dynamics of HSP70 during chronic heat stress in Tharparkar cattle. Int. J. Biometeorol. 2017;61(6):1017-1027
  7. Vaure C, Liu Y. A comparative review of toll-like receptor 4 expression and functionality in different animal species. Front. immunol. 2014;5:316
  8. Kannaki T, Shanmugam M, Verma P. Toll-like receptors and their role in animal reproduction. Anim. Reprod. Sci. 2011;125(1-4):1-12
  9. Tang D, Kang R, Coyne CB, Zeh HJ, Lotze MT. PAMP s and DAMP s: signal 0s that spur autophagy and immunity. Immunol. Rev. 2012;249(1):158-175
  10. McGuire K, Jones M, Werling D, Williams J, Glass E, Jann O. Radiation hybrid mapping of all 10 characterized bovine Toll‐like receptors. Anim. Genet. 2006;37(1):47-50
  11. Wang M, Song H, Zhu X, et al. Toll-like receptor 4 gene polymorphisms influence milk production traits in Chinese Holstein cows. Res. J. Dairy Sci. 2018;85(4):407-411
  12. Kumar S, Kumar S, Singh RV, et al. Genetic association of polymorphisms in bovine TLR2 and TLR4 genes with Mycobacterium avium subspecies paratuberculosis infection in Indian cattle population. Vet. Res. Commun. 2019;43(2):105-114
  13. Bharati J, Dangi S, Mishra S, et al. Expression analysis of toll like receptors and interleukins in Tharparkar cattle during acclimation to heat stress exposure. J. Therm. Biol. 2017;65:48-56
  14. Fang H, Wu Y, Huang X, et al. Toll-like receptor 4 (TLR4) is essential for Hsp70-like protein 1 (HSP70L1) to activate dendritic cells and induce Th1 response. JBC 2011;286(35):30393-30400
  15. Mucha R, Bhide M, Chakurkar E, Novak M, Mikula Sr I. Toll-like receptors TLR1, TLR2 and TLR4 gene mutations and natural resistance to Mycobacterium avium subsp. paratuberculosis infection in cattle. Vet. Immunol. Immunopathol. 2009;128(4):381-388
  16. Chen H, Liu C, Xiang M, et al