SavedAdd your journal

Journals / European Journal of Biology / 2023 / Cilt: 82 Sayı: 1

Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding

Yüksel GEZGİN
Gülce GÜRALP
Ayşe Berçin BARLAS
Rengin ELTEM
Pages
59–69
Publication Date
2023-06-26
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: It will be possible to make fast and low-cost molecular identification independent of sequence analysis by using primers unique to these species in the future.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Trichoderma genus are environmentally friendly, targeted biocontrol agents used in organic agriculture.
📊
Key Findings & Data:Materials and Methods: Two different gene regions (the nuclear ribosomal internal transcribed spacer (ITS) and translation elongation factor 1 (EF) were used for molecular identification of Trichoderma isolates in this study.
💡
Conclusion & Impact:Conclusion: It will be possible to make fast and low-cost molecular identification independent of sequence analysis by using primers unique to these species in the future.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:T. citrinoviride · T. atroviride · BLAST

Abstract

Objective: Trichoderma genus are environmentally friendly, targeted biocontrol agents used in organic agriculture. Currently, due to the increasing number of organic farming practices, Trichoderma species form a good market as commercial biocontrol agents. This study aims to make morphological and molecular identification of Trichoderma isolates, which were found to be potential biocontrol agents against plant pathogenic fungi, and to perform phylogenetic diversity analyses of these species using different bioinformatics tools. Materials and Methods: Two different gene regions (the nuclear ribosomal internal transcribed spacer (ITS) and translation elongation factor 1 (EF) were used for molecular identification of Trichoderma isolates in this study. Polymerase Chain Reaction (PCR) related regions were amplified and sequenced using primers specific to these gene regions. Following molecular identifications based on these two different gene regions, phylogenetic trees were drawn and polymorphic regions in the nucleotide sequences of these genes were determined. Results: As a result of the study, Trichoderma isolates were determined as T. citrinoviride Bissett and T. atroviride P. Karst. at the species level. This study not only provides essential information about the biodiversity of Trichoderma species, which is a biocontrol agent, but also allows the design of new species-specific primers based on the polymorphic regions of both species. Conclusion: It will be possible to make fast and low-cost molecular identification independent of sequence analysis by using primers unique to these species in the future.

🔖

Cite This Article

GEZGİN, Y., GÜRALP, G., BARLAS, A. B., ELTEM, R. (2023). Morphological and Molecular Identification of Trichoderma Isolates Used as Biocontrol Agents by DNA Barcoding. European Journal of Biology, Cilt: 82 Sayı: 1, 59–69. https://ajindex.com/articles/955322

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (1)

  1. Degenkolb T, Grafenhan T, Nirenberg HI, Gams W, Brückner H. Trichoderma brevicompactum complex: rich source of novel and recurrent plant-protective polypeptide antibiotics (peptaibiotics). J Agric Food Chem. 2006; 54(19): 7047-7061. google scholar 2. Degenkolb T, Dieckmann R, Nielsen KF, et al. The Trichoderma brevicompactum clade: a separate lineage with new species, new peptaibiotics, and mycotoxins. Mycol Prog. 2008a; 7(3):177-219. google scholar 3. Degenkolb T, Von Doehren H, Nielsen KF, Samuels GJ, Brück-ner H. Recent advances and future prospects in peptaibiotics, hydrophobin, and mycotoxin research, and their importance for chemotaxonomy of Trichoderma and Hypocrea. Chem Biodivers. 2008b; 5(5):671-680. google scholar 4. Maral D, Sozer S, Gezgin Y, et al. Evaluation of the properties of Trichoderma sp. isolates as a biocontrol agent and biofertilizer. Environ Eng Manag J. 2012; 11(3): S154. google scholar 5. Sargin S, Gezgin Y, Eltem R, Vardar FA. Micropropagule pro-duction from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. Turk J Biol. 2013; 37(2):139-146. google scholar 6. Gezgin Y, Gül DM, Şenşatar SS, Kara CU, Sargın S, Sukan FV. Evaluation of Trichoderma atroviride and Trichoderma citrinoviride growth profiles and their potentials as biocontrol agent and biofertilizer. Turkish J Biochem. 2020; 45(2):163-175. google scholar 7. Sharma A, Arya SK, Singh J, et al. Prospects of chitinase in sustainable farming and modern biotechnology: An update on recent progress and challenges. Biotechnol Genet Eng Rev. 2023; 1-31. google scholar 8. Lyubenova A, Rusanova M, Nikolova M, Slavov SB. Plant ex-tracts and Trichoderma spp: possibilities for implementation in agriculture as biopesticides. Biotechnol Biotechnol Equip. 2023; 37(1):159-166. google scholar 9. Salem SS. A mini review on green nanotechnology and its devel-opment in biological effects. Arch Microbiol. 2023; 205(4):128. https://doi.org/10.1007/s00203-023-03467-2. google scholar 10. Barakat I, Chtaina N, El Kamli T, Grappin P, El Guilli M, Ezzahiri B. Bioactivity of Trichoderma harzianum A peptaibols against Zymoseptoria tritici causal agent of septoria leaf blotch of wheat. J Plant Prot Res. 2023; 63(1):59-67. google scholar 11. John NS, Anjanadevi IP, Nath VS, et al. Characterization of Trichoderma isolates against Sclerotium rolfsii, the collar rot pathogen of Amorphophallus-A polyphasic approach. Biol Con-trol. 2015; 90:164-172. goog