Journals / Journal of Scientific Reports-B / 2023 / Sayı: 007

THE EFFECTS OF PLANT ORIGIN EXOSOME EXTRACTS ON A549 AND HCT116 CANCER CELL LINES

BİTKİ KÖKENLİ EKSOZOM EKSTRAKTLARININ A549 ve HCT116 KANSER HÜCRE HATLARI ÜZERİNE ETKİLERİ

Pages
46–58
Publication Date
2023-06-30
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, our study demonstrates that plant-derived exosomes may be promising therapeutic agents for future cancer treatment, and the research provides an important source for future studies.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:By applying the exosomes-like nanoparticles obtained from the leaves of the Gundelia munzuriensis plant on lung cancer (A549) and colon cancer (HCT116) cell lines, the effects of plant exosomes on cancer cells were investigated.
📊
Key Findings & Data:According to our findings, the exosomes incubated with HCT 116 cell lines affected the viability of the cancerous cells by 40-50% compared to control cells.
💡
Conclusion & Impact:As a result, our study demonstrates that plant-derived exosomes may be promising therapeutic agents for future cancer treatment, and the research provides an important source for future studies.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Nanopartiküller · HCT116 · A549

Abstract

By applying the exosomes-like nanoparticles obtained from the leaves of the Gundelia munzuriensis plant on lung cancer (A549) and colon cancer (HCT116) cell lines, the effects of plant exosomes on cancer cells were investigated. According to our findings, the exosomes incubated with HCT 116 cell lines affected the viability of the cancerous cells by 40-50% compared to control cells. On the other hand, a rate of 50% decrease in A549 cell lines was observed in 24 hours with 250 μg/mL. As a result, our study demonstrates that plant-derived exosomes may be promising therapeutic agents for future cancer treatment, and the research provides an important source for future studies.

Özet

Gundelia munzuriensis bitkisinin yapraklarından elde edilen eksozom benzeri nanopartiküller akciğer kanseri (A549) ve kolon kanseri (HCT116) hücre hatlarına uygulanarak bitki eksozomlarının kanser hücreleri üzerindeki etkileri araştırıldı. Bulgularımıza göre, HCT 116 hücre hatları ile inkübe edilen eksozomlar, kanserli hücrelerin canlılığını kontrol hücrelerine kıyasla %40-50 oranında etkilemiştir. Öte yandan, 250 μg/mL ile 24 saatte A549 hücre hatlarında %50'lik bir azalma gözlendi. Sonuç olarak, çalışmamız bitki kaynaklı eksozomların gelecekteki kanser tedavisi için umut verici terapötik ajanlar olabileceğini göstermektedir ve araştırma gelecekteki çalışmalar için önemli bir kaynak sağlamaktadır.

🔖

Cite This Article

DEMİRHAN, A., TÜRKOĞLU, S., ERMAN, F. (2023). THE EFFECTS OF PLANT ORIGIN EXOSOME EXTRACTS ON A549 AND HCT116 CANCER CELL LINES. Journal of Scientific Reports-B, Sayı: 007, 46–58. https://ajindex.com/articles/948033

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Baykara, O. (2016). Kanser tedavisinde güncel yaklaşımlar, Balıkesir Sağlık Bilimleri Dergisi, 5(3), 154-165
  2. Yang, Y. (2015). Cancer immunotherapy: harnessing the immune system to battle cancer. The Journal of clinical investigation, 125(9), 3335-3337
  3. Yıldırım, M. (2018). Antı Cancer Effect Of Garlıc Exosomes On Dıfferent Cancer Types. Master Thesis, Yeditepe University, İstanbul
  4. Vlassov, A. V., Magdaleno, S., Setterquist, R., and Conrad, R. (2012). Exosomes: current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials. Biochimica et Biophysica Acta (BBA) General Subjects, 1820(7), 940-948
  5. İnanır, C., and Ekici, L. (2020). Eksozomlar: Kompozisyonları, Biyolojik Fonksiyonları ve Biyoaktif Bileşiklerin Taşınmasındaki Potansiyelleri. Academic Food, 18(4), 421-432
  6. Kim, J., Li, S., Zhang, S., and Wang, J. (2021). Plant-derived Exosome-like Nanoparticles and their Therapeutic Activities. Asian Journal of Pharmaceutical Sciences, 1-17
  7. Zhang, W., Peng, P., Kuang, Y., Yang, J., Cao, D., You, Y., and Shen, K. (2016). Characterization of exosomes derived from ovarian cancer cells and normal ovarian epithelial cells by nanoparticle tracking analysis. Tumor Biology 37(3), 4213-4221
  8. Morton, R. E., and Evans, T. A. (1992). Modification of the bicinchoninic acid protein assay to eliminate lipid interference in determining lipoprotein protein content, Anal Biochem, 204(2), 332-4
  9. Yin, L., Wei, Y., Wang, Y., Xu, Y., and Yang, Y. (2013). Long Term and Standard Incubations of WST-1 Reagent Reflect the Same Inhibitory Trend of Cell Viability in Rat Airway Smooth Muscle Cells, Int J Med Sci., 10(1), 68–72
  10. Mathivanan, S., Ji, H., and Simpson, R. J. (2010). Exosomes: extracellular organelles important in intercellular communication. Journal of proteomics, 73(10), 1907-1920
  11. You, J. Y., Kang, S. J., and Rhee, W. J. (2021). Isolation of cabbage exosome-like nanovesicles and investigation of their biological activities in human cells. Bioactive Materials, 6(12), 4321-4332
  12. Saraç, H., Demirbaş, A., Daştan, S. D., Ataş, M., Çevik, Ö., and Eruygur, N. (2019). Evaluation of Nutrients and Biological Activities of Kenger (Gundellia tournefortii L.) Seeds Cultivated in Sivas Province. Turkish Journal of Agriculture-Food Science and Technology, 7(2), 52-58
  13. Lapa, M. R., Kırbaş, O. K., Bozkurt, B., Taşlı, P. N., Avcı, M. K., and Şahin, F. (2017). Vaccinium myrtillus (Yaban Mersini) kayn