Journals / Journal of Scientific Reports-B / 2026 / Issue: 015

Determination of some antioxidant activities in the fruit of the black mulberry (Morus nigra L.)

Karadut (Morus nigra L.) meyvesinde bazı antioksidan aktivitelerinin belirlenmesi

Mustafa Bilici(YUZUNCU YIL UNIVERSITY, VAN GÜVENLİK VOCATIONAL SCHOOL)0000-0002-8689-6463
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Publication Date
2026-04-30
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The findings revealed that black mulberry exhibits strong antioxidant activity.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This work was conducted to inspect and evaluate some known antioxidant properties of black mulberry ( Morus Nigra L. ) fruits.
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Key Findings & Data:And additionally, these fruits are strong sources of vitamins (e.g., vitamin B1 and folic acid), fatty acids (linoleic, palmitic, and oleic acids), and also melatonin.
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Conclusion & Impact:The findings revealed that black mulberry exhibits strong antioxidant activity.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Fruits · Oxidation · Antioxidant

Abstract

This work was conducted to inspect and evaluate some known antioxidant properties of black mulberry ( Morus Nigra L. ) fruits. Fruits of the Morus Genus, a plant which is a family member of the Moraceae species, are widely consumed as part of the human diet due to their beneficial effects on health. And additionally, these fruits are strong sources of vitamins (e.g., vitamin B1 and folic acid), fatty acids (linoleic, palmitic, and oleic acids), and also melatonin. It has reported that this composite structure provides versatile physiological and pharmacological benefits for human health Recent academic studies have determined high density of phenolic mixtures in black mulberry fruit, forwarding to increased interest in this fruit. One of the most important reasons for this is that the black mulberry fruit contains anthocyanins and phenolic compounds, and in addition to these, it has antimicrobial and antioxidant properties, which have led to its increasing value. Black mulberry offers a rich biochemical composition, containing various vitamins, high levels of protein, and essential mineral elements. The natural antioxidant compounds it contains: support the immune system against infectious agents, prevent anemia by suppressing hemolytic processes, inhibit the biosynthesis of carcinogenic compounds, exhibit antithrombotic effects by increasing prostaglandin synthesis, prevent atherosclerotic changes, and reduce the biochemical effects of hepatotoxic oxidant species. Various studies have reported that black mulberry can exhibit therapeutic and chemopreventive effects on certain types of cancer. The catalase (CAT) level in black mulberry samples was determined to be an average of 2.13 U/mg protein, the superoxide dismutase (SOD) level was an average of 1.14 U/mg protein, and the glutathione (GSH) level was an average of 0.16 µmol/mg. The findings revealed that black mulberry exhibits strong antioxidant activity. Accordingly, it was concluded that black mulberry may play a protective role against oxidative stress-induced cellular damage and, therefore, may be considered a potential antioxidant agent in the prevention of various chronic diseases.

Özet

Bu çalışma, kara dut (Morus Nigra L.) meyvelerinin bazı bilinen antioksidan özelliklerini incelemek ve değerlendirmek amacıyla yapılmıştır. Moraceae familyasına ait bir bitki olan Morus cinsinin meyveleri, sağlığa yararlı etkileri nedeniyle insan beslenmesinde yaygın olarak tüketilmektedir. Bu bitkilerin meyveleri, flavonoller, antosiyaninler, flavanonoller, fenolik asitler ve alkaloidler gibi çok sayıda biyoaktif bileşen ile K (potasyum), Ca (kalsiyum), Na (sodyum) ve Mg (magnezyum) gibi bazı temel mineraller içerir. Ayrıca, bu meyve vitaminler (örneğin, B1 vitamini ve folik asit), yağ asitleri (linoleik, palmitik ve oleik asitler) ve melatonin açısından da zengin kaynaklardır. Bu bileşik yapının insan sağlığı için çok yönlü fizyolojik ve farmakolojik faydalar sağladığı bildirilmiştir. Son akademik çalışmalar, kara dut meyvesinde yüksek yoğunlukta fenolik karışımlar olduğunu belirlemiş ve bu meyveye olan ilgiyi artırmıştır. Bunun en önemli nedenlerinden biri, siyah dut meyvesinin antosiyaninler ve fenolik bileşikler içermesi ve bunlara ek olarak antimikrobiyal ve antioksidan özelliklere sahip olmasıdır, bu da meyvenin değerinin artmasına neden olmuştur. Kara dut, güçlü bir antioksidan deposudur. Kara dut, çeşitli vitaminler, yüksek protein seviyeleri ve temel mineral elementler içeren zengin bir biyokimyasal bileşime sahiptir. İçerdiği doğal antioksidan bileşikler: enfeksiyon etkenlerine karşı bağışıklık sistemini destekler, hemolitik süreçleri baskılayarak anemiyi önler, kanserojen bileşiklerin biyosentezini inhibe eder, prostaglandin sentezini artırarak antitrombotik etkiler gösterir, aterosklerotik değişiklikleri önler ve hepatotoksik oksidan türlerinin biyokimyasal etkilerini azaltır. Çeşitli çalışmalar, kara dutun belirli kanser türleri üzerinde terapötik ve kemopreventif etkiler gösterebileceğini bildirmiştir. Kara dut örneklerindeki katalaz (CAT) seviyesi ortalama 2,13 U/mg protein, süperoksit dismutaz (SOD) seviyesi ortalama 1,14 U/mg protein ve glutatyon (GSH) seviyesi ortalama 0,16 µmol/mg olarak belirlenmiştir. Bulgular, kara dutun güçlü bir antioksidan aktivite sergilediğini ortaya koymuştur. Buna göre, kara dutun oksidatif stresin neden olduğu hücresel hasara karşı koruyucu bir rol oynayabileceği ve bu nedenle çeşitli kronik hastalıkların önlenmesinde potansiyel bir antioksidan ajan olarak düşünülebileceği sonucuna varılmıştır.

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Cite This Article

Bilici, M. (2026). Determination of some antioxidant activities in the fruit of the black mulberry (Morus nigra L.). Journal of Scientific Reports-B, Issue: 015. https://ajindex.com/articles/1619076

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References (61)

  1. S. Kolayli, E. Asadov, A. Huseynovauseynova, S. Rahimova, Y. Kara1,’’ Phenolic composition and antioxidant properties of black mulberry (Morus nigra l.) fruits and leaves’’ Journalof Wildlife and Biodiversity. 8(2):355 -364. 2024.
  2. L. De Santana, I. Henrıque, P. Andrade, S. P. Dos Santos, C. O. De Souza, C. D.F. Renato and S. Cruz. ‘’Prospection on the black mulberry (Morus nigra L.): A technological and scientific analysis’’ Chemıcal Scıences.96(4): PP-1-19. 2024.
  3. D. Özbalcı, E. Aydın, G. Özkan, ‘’The phytochemical profile and pharmacological properties of mulberry fruit’’. Food and Health, 9(1), 69-86. 2023.
  4. R. Meral and İ. S. Doğan, "Antioxidant Activity and Phenolic Composition of Black Mulberry (Morus nigra) Enriched Bread," Iğdır University Journal of Science, vol.2, no.4, pp.43-48, 2012.
  5. J. Huo, Y. Ni, D. Li, J. Qiao, D. Huang, X. Sui and Y. Zhang. ‘’ Comprehensive structural analysis of polyphenols and their enzymatic inhibition activities and antioxidant capacity of black mulberry (Morus nigra L.)’’ Food Chemistry. 427: Pp-1-9. 2023.
  6. Y. Elmaci and T. Altug, ‘’Flavour evaluation of three black mul berry (Morus nigra) cultivars using GC/MS, chemical and sensory data,’’ Journal of the Science of Food and Agriculture, 82: 632-635. 2002.
  7. E. Sakar, S. Ercisli, A. M. Romina, H. Gulen, A. Assouguem,*, R. Ullah, A. Shahat, A. Bari and A. Farah, ‘’ Black mulberry (Morus nigra L.) fruits: As a medicinal plant rich in human health-promoting compounds’’ Open Chemistry. 21:Pp: 1-11. 2023.
  8. P.N.Chen, S.C. Chu, HL.Chipu, WH. Kuo, CL. Chiang AND YS. Hsieh, ‘’Mulberry Anthocynins, Cynidin-3 rutinoside, Cynidin-3-glucoside, Exhibited an Inhibitory Effect on the Migration and Invasion of a Human Lung Cancer Cell Line.’’ Cancer Lett 235(2): 248-259. 2006.
  9. S.H. Bae AND H.J, Suh, ‘’Antioidant Activities of Five Different Mulberry Cultivars in Korea,’’ Food Scie Technol 40(6): 955-962. 2007.
  10. T. Baytop, ‘’Plant-Based Medicine in Turkey: Past and Present,’’ Nobel Medical Publications, 2nd Edition, Istanbul,480. 1999.
  11. M. Abay and N. Eruygur, ‘’Investigation of Antioxidant Activities of Fruit, Leaf, and Branche Extracts of White (Morus alba L.) and Black (Morus nigra L.) Mulberry Species from Diyarbakır’’ Türkiye Tarımsal Araştırmalar Dergisi. 8(2): 177-183. 2021.
  12. A. Güner, N. Özhatay, T. Ekim, K.H.C. Başer, ‘’Flora of Turkey and the East Aegean Islands’' Lathyrus Publisher: Edinburgh at the university press. Vol. 11 (pp.92-94), 2000.
  13. N. Güngör and M. Sengül, ‘’Antioxidant activity, total pheno lic content and selected physicochemical properties of white mulberry (Morus alba L.) fruits,’’ International Journal of Food Properties, 11: 44-52. 2008.
  14. A.A. Polat, ‘’Determination of fruit characteristics of certain mulberry varieties cultivated in Antakya district of Hatay,’’ Garden, 33 (1-2): 67-73. 2004.
  15. M. Grieve, Mulberry common. http: //botanical. com/botani cal/ mgmh/m/ mul.com62. (Erişim tarihi: 10.08.2009). 2002.
  16. A. Asımgil, ‘’Medicinal plants,’’ Timaş Publications, Istanbul. 1997.
  17. Y.Ö. Alifakı, Ö. Şakıyan and A. İşci, ‘’ Investigation of dielectric properties, total phenolic content and optimum formulation of microwave baked gluten-free cakes,’’ Journal of Food Science and Technology, 56(3), 1530 1540. 2019. doi: 10.1007/s13197-019-03647-3.
  18. R. Meral, "Effect of Mulberry Fortification on Functional, Physical and Sensory Properties of Gluten-free Muffin,"Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.27, no.3, pp.557-569, 2022.
  19. G.Y. Park, Q. Liu, J.S. Hong and H.J. Chung,‘’Anti-staling and quality characteristics of Korean rice cake affected by mulberry (Morus alba L.) leaf powder fortification.’’ Journal of Cereal Science, 97, 103133. 2021. doi: 10.1016/j.jcs.2020.103133.
  20. H. Zhang, Z.F. Ma, X. Luo and X. Li,‘’Effects of mulberry fruit (Morus alba L.) consumption on health outcomes,’’ A mini-review. Antioxidants, 7(5), 69. 2018.
  21. B. Aşkın, Ş. Tağı and E. Küçüköner, ‘’İşleme ve Isıl İşlem Sırasında Karadut (Morus nigra) Suyunun Antibakteriyal ve Antifungal Aktivitesi,’’ KSÜ Tarım ve Doğa Derg 25 (6): 1414-1422. 2022.
  22. Y. Cao, Z.F. Ma, H. Zhang, Y. Jin, Y. Zhang and F. Hayford, ‘’Phytochemical properties and nutrigenomic implications of yacon as a potential source of prebiotic: Current evidence and future directions.’’ Foods, 7(4), 59. 2018.
  23. Z. Feei Ma, H. Zhang, ‘’Phytochemical constituents, health benefits, and industrial applications of grape seeds’’ A mini-review. Antioxidants, 6(3), 71. 2017.
  24. D. Özbalcı, E. Aydın, G. Özkan, ‘’The phytochemical profile and pharmacological properties of mulberry fruit.’’ Food and Health, 9(1), 69-86. 2023.
  25. C. Veeresham, ‘’Natural products derived from plants as a source of drugs.’’ Journal of Advanced Pharmaceutical Technology & Research, 3(4), 200. 2012.
  26. A. Gryn-Rynko, G. Bazylak and D. Olszewska-Slonina, ‘’New potential phytotherapeutics obtained from white mulberry (Morus alba L.) leaves.’’ Biomedicine & Pharmacotherapy, 84, 628-636. 2016.
  27. C.C. Chen, L.K. Liu, J.D. Hsu, H.P. Huang, M.Y. Yang and C.J. Wang, ‘’Mulberry extract inhibits the development of atherosclerosis in cholesterol-fed rabbits.’’ Food Chemistry, 91(4), 601-607. 2005.
  28. Q.Z. Du, J.F. Zheng, Y. Xu, ‘’Composition of anthocyanins in mulberry and their antioxidant activity.’’ Journal of Food Composition and Analysis, 21(5), 390-395. 2008.
  29. S. Ercisli and E. Orhan, ‘’Chemical composition of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry fruits.’’ Food Chemistry, 103(4), 1380-1384. 2007.
  30. M.A. Khan, A.A. Rahman, S. Islam, P. Khandokhar, S. Parvin, M.B. Islam, M. Hossain, M. Rashid, G. Sadik and S. Nasrin, ‘’A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae).’’ BMC Research Notes, 6(1), 1-9. 2013.
  31. D. Ning, B. Lu and Y. Zhang, ‘’The processing technology of mulberry series product.’’ China Fruit Veg. Process, 5, 38-40. 2005.
  32. X. Yang, L. Yang and H. Zheng, ‘’Hypolipidemic and antioxidant effects of mulberry (Morus alba L.) fruit in hyperlipidaemia rats.’’ Food and Chemical Toxicology, 48(8 9), 2374-2379. 2010.
  33. H. Wei, H. Liu, S. Liao, Y. Ma, C. Wang, D. Tong, J. Feng, J. Yi, T. Zhu, ‘’A systematic review of the medicinal potential of mulberry in treating diabetes mellitus.’’ The American journal of Chinese medicine, 46(08), 1743 1770. 2018.
  34. A. Can, A. Kazankaya, E. Orman, M. Gundogdu, S. Ercisli, R. Choudhary, R. Karunakaran, ‘’Sustainable mulberry (Morus nigra L., Morus alba L. and Morus rubra L.) production in Eastern Turkey.’’ Sustainability, 13(24), 2021. 13507.
  35. S. Ercisli, ‘’A short review of the fruit germplasm resources of Turkey.’’ Genetic Resources and Crop Evolution, 51(4), 419-435. 2004.
  36. Y. Wang, L. Xiang, C. Wang, C. Tang, X. He, ‘’Antidiabetic and antioxidant effects and phytochemicals of mulberry fruit (Morus alba L.) polyphenol enhanced extract,’’ PLoS One, 8(7), e71144. 2013.
  37. G. Hojjatpanah, M. Fazaeli, Z. Emam‐Djomeh, ‘’Effects of heating method and conditions on the quality attributes of black mulberry (Morus nigra) juice concentrate.’’ International Journal of Food Science & Technology, 46(5), 956-962. 2011.
  38. B. Jan, R. Parveen, S. Zahiruddin, M.U. Khan, S. Mohapatra and S. Ahmad, ‘’Nutritional constituents of mulberry and their potential applications in food and pharmaceuticals’’ Saudi Journal of Biological Sciences, 28(7), 2021. 3909-3921.
  39. M. Sengül, M.F. Ertugay and M. Sengül, ‘’Rheological, physical and chemical characteristics of mulberry pekmez,’’ Food Control, 16(1), 73-76. 2005.
  40. D. Bhattacharjya, A. Sadat, P. Dam, D.F. Buccini, R. Mondal, T. Biswas, K. Biswas, H. Sarkar, A. Bhuimali, A. Kati, ‘’Current concepts and prospects of mulberry fruits for nutraceutical and medicinal benefits.’’ Current Opinion in Food Science, 40, 121-135. 2021.
  41. Y.M. Lee, Y. Yoon, H. Yoon, H.M. Park, S. Song and K.J. Yeum, ‘’Dietary anthocyanins against obesity and inflammation.’’ Nutrients, 9(10), 1089. 2017.
  42. G. Ma, X. Chai, G. Hou, F. Zhao, Q. Meng, ‘’Phytochemistry, bioactivities and future prospects of mulberry leaves:’’ A review. Food Chemistry, 372, 2022. 131335.
  43. G. Kusano, S. Orihara, D. Tsukamoto, M. Shibano, M. Coskun, A. Guvenc, C.S. Erdurak, ‘’Five new nortropane alkaloids and six new amino acids from the fruit of Morus alba L INNÉ growing in Turkey.’’ Chemical and Pharmaceutical Bulletin, 50 (2), 185-192. 2002.
  44. J. Yang, X. Liu, X. Zhang, Q. Jin and J. Li, ‘’ Phenolic profiles, antioxidant activities, and neuroprotective properties of mulberry (Morus atropurpurea Roxb.) fruit extracts from different ripening stages.’’ Journal of Food Science, 81(10), 2016. C2439-C2446.
  45. M. Arfan, R. Khan, A. Rybarczyk and R. Amarowicz, ‘’Antioxidant activity of mulberry fruit extracts.’’ International Journal of Molecular Sciences, 13(2), 2012. 2472 2480.
  46. E.W.C. Chan, L. Phui-Yan, W. Siu-Kuin, ‘’Phytochemistry, pharmacology, and clinical trials of Morus alba.’’ Chinese Journal of Natural Medicines, 14(1), 17-30. 2016
  47. M. Imran, H. Khan, M. Shah, R. Khan, F. Khan, ‘’Chemical composition and antioxidant activity of certain Morus species.’’ Journal of Zhejiang University Science B, 11(12), 973-980. 201).
  48. W. Chen, Y. Li, T. Bao and V. Gowd,‘’Mulberry fruit extract affords protection against ethyl carbamate-induced cytotoxicity and oxidative stress.’’ Oxidative Medicine and Cellular Longevity, 2017.
  49. S.Y. Wang and H. Lin, ‘’Antioxidant Activity in Fruits and Leaves of Blackberry, Raspberry and Strawberry Varies with Cultivar and Developmental Stage.’’ Journal of Agriculture Food Chemistry 48(200):140-146. 2000.
  50. M. Pehluvan and M. Güleryüz, ‘’The Importance of Raspberries and Blackberry for Human Health.’’ Garden 33(1–2):51–57. 2004.
  51. S. Benvenuti, F. Pellati, M. Melegari and D. Bertelli, ‘’Polyphenols, Anthocyanins, Ascorbic Acid and Radical Scavenging Activity of Rubus, Ribes and Aronia.’’ Journal of Food Science 69(3):164–169. 2004.
  52. W. Aneta, O. Jan, M. Magdalena and W. Joanna, ‘’Phenolic Profile, Antioxidant and Antiproliferative Activity of Black and Red Currants (Ribes sp.) From Organic and Conventional Cultivation.’’ International Journal of Food Science and Technology 48:715–726. 2013.
  53. M. Bilici. ‘’The Effect of Currant (Ribes) on Human Health and Determination Certain Antioxidant Activities.’’ East J Med 26(3): 470-474. 2021.
  54. J. Tabart, T. Franck, C. Kevers, J. Pincemail, D. Serteyn, J. O. Defraigne and J. Dommes, ‘’Antioxidant and Anti–inflammatory Activities of Ribes nigrum Extracts.’’ Food Chemistry 131(4):1116–1122. 2012.
  55. L. Pecko J. Takac, J. Cvopa, K. Smolarz and K. Zmarlicki, ‘’Nutrient Contents in Fresh and Processed Currant Fruits, Srict International Symposium on Rubus and Ribes,’’ Acta Horticulturae No:352,205-208p, 1993.
  56. B. Radovanovic, A. Milenkovic-Andjelkovic, A. Radovanovic and M. Andjelkovic, ‘’HPLC Analysis of Phenolic Compounds and Antioxidant Activity of Ribes Fruit Leaves.’’ XX Savetovanje O Biotehnologiji 20(22):341–346. 2015.
  57. G. Gençaslan, ‘’Screening of Antioxidant Properties of Some Plants Used for Medical Purposes in Turkey (Master's Thesis).’’ Ankara University Institute of Health Sciences Department of Pharmacy and Pharmacology, Ankara, 102 s. 2007.
  58. H.Wang, H. Cao and R.L. Prior ‘’Oxygen Radical Absorbing Capacity of Anthocyanins. J. Agric. Food Chem. 45: 304-309. 1997.
  59. I.M. Heinonen, A.S. Meyer and E.N. Frankel ‘’Antioxidant Activity of Berry Phenolics on Human Low-Density Lipoprotein and Liposome Oxidation.’’ J. Agric. Food Chem. 46: 4107-4112. 1998.
  60. R. Meral, "Effect of Mulberry Fortification on Functional, Physical and Sensory Properties of Gluten-free Muffin,"Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.27, no.3, pp.557-569, 2022.
  61. D. Bhattacharjya, A. Sadat, P. Dam, D.F. Buccini, R. Mondal, T. Biswas, K. Biswas, H. Sarkar, A. Bhuimali, A. Kati, ‘’Current concepts and prospects of mulberry fruits for nutraceutical and medicinal benefits.’’ Current Opinion in Food Science, 40, 121-135. 2021.