Journals / Van Sağlık Bilimleri Dergisi / 2006 / Cilt: 9 Sayı: 1

Detection and characterization of rotaviruses in domestic pigeons Columba liviaj using polyacry lamide gel electrophoresis PAGE technique

Evcil güvercinlerde Columba livicî rotavirusların polyacrylamide gel electrophoresis PAGE tekniği ile tanısı ve karakterizasvonu

Pages
107–115
Publication Date
2006-01-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This report is the fırst detection of rotaviruses in faecal specimens from domestic pigeons in Turkev.

📌 3 Key Takeaways

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Objective & Hypothesis:This research was conductea to determine the presence of rotaviruses in domestic pigeons using polyacrylamide gel electrophoresis PAGE silver staining.
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Key Findings & Data:In this research, 323 faecal specimens that belong to 289 cl inically healthy and 34 diarrhoeic pigeons were tested by PAGE silver staining technique.
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Conclusion & Impact:This report is the fırst detection of rotaviruses in faecal specimens from domestic pigeons in Turkev.
🔬Design:Research Article
📍Scope:22 samples
⚙️Variables:Evcil güvercin. PAGE. Rotavirus · Domestic pigeon. PAGE. Rotavirus

Abstract

This research was conductea to determine the presence of rotaviruses in domestic pigeons using polyacrylamide gel electrophoresis PAGE silver staining. Furthermore. the rotaviruses determined in this study were distinguished according to their migration patterns. Rotaviruses are established as cause of gastroenteritis especially in the young of mamals and birds species. and give rise to both symptomatic and asymptomatic infections. In this research, 323 faecal specimens that belong to 289 cl inically healthy and 34 diarrhoeic pigeons were tested by PAGE silver staining technique. At the result. 22 samples 7.61 % taken from healthy pigeons and 11 samples 32.35 % from diarrhoeic pigeons were detected as positive for rotavirus. Positive specimens detected were characterized as typical rotavirus according to pattern of 11 RNA segments. and tlıree different electrophoretypes were detected. This report is the fırst detection of rotaviruses in faecal specimens from domestic pigeons in Turkev.

Özet

Bu araştırma evcil güvercinlerin dışkı örneklerinde rotavirusların polyacrylamide gel electrophoresis PAGE gümüş boyama tekniği kullanılarak belirlenmesi amacıyla gerçekleştirildi. Ayrıca, çalışmada tespit edilen rotavirusların elektro foretik tip karakterizasvonu yapılarak aralarındaki farklılıklar belirlendi. Rotaviruslar memeli ve kanatlı hayvan türlerinin özellikle gençlerinde gastroenteritise neden olar. mikroorganizmalardır ve bu etkenler semptomatik ve asemptomatik enfeksiyonlar meydana getirirler. Bu araştırmada, sağlıklı görünümlü 289 ve ishal semptomu gösteren 34 güvercinden alınan toplam 323 dışkı örneği PAGE gümüş boyama tekniği ile incelendi. Araştırma sonucunda, sağlıklı görünümlü güvercinlerden 22 % 7.61 'si ve ishal semptomlu güvercinlerden 11 %32.35 'i rotavirus yönünden pozitif bulundu. Pozitif tespit edilen örneklerin 11 RNA segmentine sahip rotavirus oldukları belirlendi ve migrasyon kalıplarına göre üç farklı elektroforetik tip tespit edildi. Bu araştırma ile Türkiye'de evcil güvercinlerde rotavirusların varlığı ilk kez ortaya konuldu

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Cite This Article

ASLAN, S., ÇABALAR, M. (2006). Detection and characterization of rotaviruses in domestic pigeons Columba liviaj using polyacry lamide gel electrophoresis PAGE technique. Van Sağlık Bilimleri Dergisi, Cilt: 9 Sayı: 1, 107–115. https://ajindex.com/articles/947722

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References (3)

  1. 1 Fenner JF. Gibbs JPE. Murphy AF. Rott R.Studdent JM. White OD: Reoviridea. Veterinarv Virology. 2nd Ed. Academic Press.
  2. INC. London. (1993). 2 Donelli G. Superti F: The rotavirus genus. CompImmun Microbiol Infect Dis. 17: 305-320. (1994). 3 McNulty MS: Rotavirus infections, Virüsinfections of birds. Editors, JB McFerran and MS McNulty. Elsevier Science Publishers B. V. Amsterdam. (1993). 4 Burgu İ. Akça Y. Alkan F. Özkul A. Karaoğlu T:Yenidoğan ishalli buzağılarda rotavirusların elektron mikroskopi (EM), enzvme linked immunosorbent assay (ELISA) ve polyacrylamide gel electrophoresis (PAGE) teknikleri ile çabuk teşhisi ve antijenik karakterizasvonu. Ankara Üniv Vet Fak Dere. 42:491-498.(1995). 5 Burgu İ. Akça Y. Alkan F. Özkul A. Karaoğlu T.Yesilbağ K. Oğuzoğlu TÇ. Dağalp SB. Tan MT: Klinik olarak sağlıklı veya diyare semptomlu koy unlarda rotavirus enfeksiyonunun enzvme linked immunosorbent assay (ELISA) ve polyacrylamide gel electrophoresis (PAGE) teknikleri ile araştırılması. Ankara Üniv Vet FakDerg.46:143-147.(1999). 6 Özkul A. Yeşilbağ K. Karaoğlu T. Burgu İ:Electrophoretypes of bovine rotaviruses detected in Turkev. Türk J Vet Anim Sci. 26: 359- 362.(2002). 7 Huq MI. Rahman ASMM. Al-sadiq A. Al-sahri A. Alim ARMA: Rotavirus as an important cause of diarrhoea in a hospital for children in Danımam, Saudi Arabia. Ann Trop Pediatr. 7: 173-176. (1987). 8 Bulgin MS, Ward AC. Barret DP. Lane VM:Detection of rotavirus and coronavirus shedding in two beef cow herds in Idaho. Can VetJ. 30:235-239. (1989). 9 Hoshino Y. Wvatt RG. Greenberg HB. Flores J,Kapikian AZ: Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied bv plaque- reduction neutralization. J Infect Dis. 149: 694-702.(1984). 10 Reynolds DL. Saif YM. Theil KW: A surveyof enteric viruses of turkev poults. Avian Dis. 31:89-98.(1987). HHerring AJ. Inglis HF. Ojeh CK. Snodgrass RD. Menzies JD: -Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver stained polvacrvlamide gels. J Clin Microbiol. 16: 473-477.(1982). 12 Çabalar M. Voyvoda H. Sekin S: Virologicaland serological examinations for rotaviruses in diarrhoeic calves, YYÜ Vet FakDerg.il: 18-21.(2000). 13 BoynukaraB. Çabalar M. GtilhanT. Aslan S:Prevalence of Esclıerichia coli K99 and rotavirus in Turkish Van Cats, British Small Animal Veterinary Association (BSAVA) 44' Annual Cogress. Scientifıc Proceedings 5-8
  3. April. ICC Birmingham. UK.(2001). 14 McNulty MS. Logan EF: Lon