Journals / Türkiye Entomoloji Dergisi / 2015 / Cilt: 39 - Sayı: 1

Effects of pyriproxyfen on bioenergetic resources of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

Pyriproxyfenin Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyoenerji kaynakları üzerine etkileri

Pages
11–22
Publication Date
2015-03-13
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Only the protein level showed a significant difference by sex.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Colorado potato beetles (CPB) overwinters as adults in the soil, thus, their survival is related with their energy reserves.
📊
Key Findings & Data:The present study examined the biochemical changes in adults treated with sub-lethal concentrations (≤LC30) of pyriproxyfen.
💡
Conclusion & Impact:Only the protein level showed a significant difference by sex.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Patates böceği · karbonhidratlar · proteinler

Abstract

Colorado potato beetles (CPB) overwinters as adults in the soil, thus, their survival is related with their energy reserves. The present study examined the biochemical changes in adults treated with sub-lethal concentrations (≤LC30) of pyriproxyfen. First, both the plants and overwintered adults were sprayed with different levels of pyriproxyfen (0, 250, 500 and 750 µl/l) under field conditions and were tested at 3, 6 and 12 days after treatment. Next, the adults from the first experiments were re-sprayed 1 week later with pyriproxyfen at the same concentrations and were re-sampled at 3, 6 and 12 days after the second treatment. The lipid, sugar, glycogen and protein levels of 4 males and 4 females were determined (mg/g; w/w). Results revealed a significant decline in lipid (87.7%), glycogen (50%) and caloric contents (75%) levels with respect to controls. Sugar and protein levels increased 7.89 and 5.79 times with respect to the controls. The best results were obtained at 250 µl/l of pyriproxyfen. The second round of testing demonstrated the additive effects of pyriproxyfen on bioenergetic reserves. Only the protein level showed a significant difference by sex.

Özet

Patates böceği, toprakta ergin olarak kışlamaktadır. Bu nedenle, onların hayatta kalmaları enerji rezervleri ile ilişkilidir. Bu çalışmada pyriproxyfenin alt öldürücü konsantrasyonları (≤LC30) uygulanmış erginlerdeki biyokimyasal değişiklikler incelenmiştir. İlk olarak, bitki ve kışlaktaki erginlere tarla şartlarında pyriproxyfen (0, 250, 500 ve 750 µl/l) farklı düzeylerde püskürtülmüş ve uygulamadan 3, 6 ve 12 gün sonra test edilmiştir. Birinci deneyden alınan erginlere 1 hafta sonra tekrar aynı konsantrasyonlarda pyriproxyfen uygulanmış ve 3, 6 ve 12 gün sonunda yeniden örneklenmiştir. 4 erkek ve 4 dişi bireyde lipit, şeker, glikojen ve protein düzeyleri (mg/g; a/a) tespit edilmiştir. Sonuçlar, kontrole göre lipid (% 87.7), glikojen (% 50) ve kalori içeriği (% 75) düzeylerinde önemli bir düşüş göstermiştir. Şeker ve protein düzeyleri kontrole göre 7.89 ve 5.79 kat artmıştır. En iyi sonuçlar, pyriproxyfenin 250 µl/l dozunda elde edilmiştir. Testin ikinci turu pyriproxyfenin biyoenerjik rezervler üzerindeki ilave etkilerini göstermiştir. Sadece protein düzeyi cinsiyete göre anlamlı bir farklılık göstermiştir.

🔖

Cite This Article

FOTOUHİ, K., FAZEL, M., KAVOUSİ, A. (2015). Effects of pyriproxyfen on bioenergetic resources of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae). Türkiye Entomoloji Dergisi, Cilt: 39 - Sayı: 1, 11–22. https://ajindex.com/articles/93207

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Amer, M.S., 1990. Effects of the anti-JH synthesis (Mevalonic acid) on the main metabolites of Spodoptera littoralis. Egyptian Journal of Applied Science. 5: 82-91.
  2. Ascher, K.R.S. & M. Eliyahu, 1988. The ovicidal properties of the juvenile hormone mimic sumitomo S-31183 (SK- 591) to insects. Phytoparasitica. 16: 15-21.
  3. Bagheri, F., K.H.Talebi & V. Hosseininaveh, 2010. Cellular energy allocation of pistachio green stink bug, Brachynema germari Kol. (Hemiptera.: Pentatomidae) in relation to juvenoid pyriproxyfen. African Journal of Biotechnology. 9: 5746-5753.
  4. Bailey, E., J.A. Horne, M.E.G. Izatt & L. Hill, 1975. the effects of allatectomy on the lipid composition of the fat body and hemolymph of adult Locusta. Comparative Biochemistry and Physiology. 52B: 525-528.
  5. Bais, V.S. & T. Arasta, 1995. Effect of sublethal concentration of aldrex on protein, lipid and glycogen level in the liver and muscle of catfish, Mystus vittatus. Journal of Freshwater Biology. 7: 151-154.
  6. Beenakkers, A.M.T.H., D.J. Van Der Horst & W.J. Van Marrewijk, 1981. “Role of Lipids in Energy Metabolism, 53-100”. In: Energy Metabolism in Insects (Ed: R. G. H. Downer), Plenum Press, New York, 244 p. Bosch, J. & W.P. Kemp, 2004. Effect of pre-wintering and wintering temperature regimes on weight loss, survival, and emergence time in the mason bee Osmia cornuta (Hymenoptera: Megachilidae). Apidologie. 35: 469-479.
  7. Breccia, A., E. Gattavecchia, G. Albonetti & A.M. Dipietra, 1976. Radiobiochemistry of phytodrugs: I. role of juvenile hormones and analogs in the biosynthesis of proteins and RNA in Drosophila larvae. Journal of Environmental Science and Health. 11: 1-7.
  8. Chapman, R.F., 1982.
  9. The Insects, Structure and Function. Harvard University Press,
  10. Cambridge, MA, USA. Cotton, G., 1989. A study of the effects of the juvenile hormone analogue methoprene on the intermediary metabolism of the African migratory locust, Durham theses, Durham University. Available at Durham E-Theses Online: http://etheses.dur.ac.uk/6432/.
  11. Cymborowski, B., 1992. Insect Endocrinology. Polish Scientific Publisher. 234 p. De Kort, C.A.D. & A.B. Koopmanschap, 1990. “Effects of a Juvenile Hormone Analogue on Development, Dhadialla, T.S., G.R. Carlson & D.P. Le, 1998. New insecticides with ecdysteroidal and juvenile hormone activity. Annual Review of Entomology. 43: 545-569.
  12. Downer, R.G.H., 1985. “Lipid Metabolism, 75–114”. In: Comprehensive Insect Physiology, Biochemistry, and Pharmacology. (Eds.: G.A. Kerkut & L.I. Gilbe