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Journals / Türkiye Entomoloji Dergisi / 2014 / Cilt: 38 - Sayı: 4

Expression levels of glutamate and serotonin receptor genes in the brain of different behavioural phenotypes of worker honeybee (Apis mellifera)

Farklı davranış fenotiplerinde işçi bal arısı (Apis mellifera), beyninde glutamat ve serotonin reseptör genlerinin ekspresyon düzeyleri

Qiu-hong QIN
Xu HAN
Hao LIU
Shao-wu ZHANG
Zhi-jiang ZENG
Pages
431–441
Publication Date
2014-08-01
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Our results showed that: the relative expression level of NMDAR genes was much higher in newly emerged workers (NW) than in young nurses (YN) and “old” foragers (OF) (P 0.05), but there were no significant differences among NW, YN, and OF for the relative expression level of mGluR1 and mGluR4 gene (P > 0.05); in the case of Serotonin (5-HT), the relative expression level of the 5-HT1 gene showed no significant difference between YN and OF (P > 0.05), but was higher in YN and OF than in NW (P

📌 3 Key Takeaways

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Objective & Hypothesis:Social insect colonies are known for their efficient system of task specialization.
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Key Findings & Data:The glutamate receptor genes include the ionotropic glutamate receptor (iGluR) genes and metabotropic glutamate receptor (mGluRs) genes, and has 9 orthologous genes in honeybee, such as the N-methyl-D-aspartate receptor (NMDAR) genes, NMDAR1 and NMDAR2, the α-amino-3-hydroxy-5-methyl-4isoxazole receptor (AMPAR) genes, AMPAR ∆2-a, AMPAR ∆2-b, AMPAR ∆2-c, AMPAR ∆2-d, and the mGluR1, mGluR4 and mGluR7.
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Conclusion & Impact:Our results showed that: the relative expression level of NMDAR genes was much higher in newly emerged workers (NW) than in young nurses (YN) and “old” foragers (OF) (P 0.05), but there were no significant differences among NW, YN, and OF for the relative expression level of mGluR1 and mGluR4 gene (P > 0.05); in the case of Serotonin (5-HT), the relative expression level of the 5-HT1 gene showed no significant difference between YN and OF (P > 0.05), but was higher in YN and OF than in NW (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Apis mellifera · yaş · beyin

Abstract

Social insect colonies are known for their efficient system of task specialization. In this study, we analyzed the brain expression level of glutamate and serotonin receptor genes in different behavioural phenotypes of (Apis mellifera) workers by qRT-PCR. The glutamate receptor genes include the ionotropic glutamate receptor (iGluR) genes and metabotropic glutamate receptor (mGluRs) genes, and has 9 orthologous genes in honeybee, such as the N-methyl-D-aspartate receptor (NMDAR) genes, NMDAR1 and NMDAR2, the α-amino-3-hydroxy-5-methyl-4isoxazole receptor (AMPAR) genes, AMPAR ∆2-a, AMPAR ∆2-b, AMPAR ∆2-c, AMPAR ∆2-d, and the mGluR1, mGluR4 and mGluR7. Our results showed that: the relative expression level of NMDAR genes was much higher in newly emerged workers (NW) than in young nurses (YN) and “old” foragers (OF) (P 0.05), but there were no significant differences among NW, YN, and OF for the relative expression level of mGluR1 and mGluR4 gene (P > 0.05); in the case of Serotonin (5-HT), the relative expression level of the 5-HT1 gene showed no significant difference between YN and OF (P > 0.05), but was higher in YN and OF than in NW (P

Özet

Sosyal böcekler görev dağılımının en bilinen sistemleridir. Bu çalışmada, qRT-PCR ile bal arası (Apis mellifera) işçilerinin farklı davranış fenotiplerinde beyinde bulunan glutamat ve serotonin reseptör genlerinin ekspresyon seviyeleri analiz edilmiştir. Glutamat reseptör geni; İyonotropik glutamat reseptörü (iGluR) ve metabotropik glutamat reseptörü (mGluR'ler) genlerini kapsar. Balarısında Glutamat reseptör geni, N-metil-D-aspartat reseptör (NMDAR) genleri, NMDAR1 ve NMDAR2, α-amino-3-hidroksi-5-metil-4-izoksazol reseptörü (AMPAR) genleri, AMPAR ∆2-a AMPAR ∆2-b AMPAR ∆2-c AMPAR ∆2-d ve mGluR1, mGluR4 ve mGluR7 olmak üzere 9 ortolog gene sahiptir. Sonuçlarımıza göre: NMDAR geninin ekspresyon düzeyi yeni çıkış yapmış işçilerde (NW), genç (YN) ve yaşlı bireylere (OF) oranla çok daha yüksek bulunmuşken (p0.05); genç, yaşlı ve işçi arıların hepsinde mGluR1 ve mGluR4 genlerinin ekspresyon düzeyleri açısından (p>0.05) anlamlı bir fark bulunamamıştır. Serotonin (5-HT) söz konusu olduğunda, 5-HT1 geninin ekspresyon seviyesi bakımından genç ve yaşlı bireyler arasında anlamlı bir fark çıkmamıştır (P>0.05) ancak aynı genin ifadesi genç ve yaşlı bireylerde işçi arılara göre daha yüksek bulunmuştur (P

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Cite This Article

QIN, Q., HAN, X., LIU, H., ZHANG, S., ZENG, Z. (2014). Expression levels of glutamate and serotonin receptor genes in the brain of different behavioural phenotypes of worker honeybee (Apis mellifera). Türkiye Entomoloji Dergisi, Cilt: 38 - Sayı: 4, 431–441. https://ajindex.com/articles/93133

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References (11)

  1. Barbas, D., L. Desgroseillers, V.F. Castellucci, T.J. Carew & S. Marinesco, 2003. Multiple serotonergic mechanisms contributing to sensitization in Aplysia: evidence of diverse serotonin receptor subtypes. Learnning & Memory 10: 373–386.
  2. Barbas, D., A. Campbell, V.F. Castellucci & L.L. Desgroseillers, 2005. Comparative localization of two serotonin receptors and sensor in the central nervous system of Aplysia californica. Journal of Comparative Neurology 3: 295–304.
  3. Barnes, N.M. & T. Sharp, 1999. A review of central 5-HT receptors and their function. Neuropharmacology 38: 1083- 1152. Bear, M.F., 1999. Homo synaptic long-term depression, a mechanism for memory? Proceedings of the National academy of Sciences of the United States of America 96: 9457–9458.
  4. Bliss, T.V. & G.L. Collingrodge, 1993. A synaptic model of memory: long-term potentiation in the hippocampus. Nature 361: 31–39.
  5. Bicker, G., 1999. Histochemistry of classical neurotransmitters in antennal lobes and mushroom bodies of the honeybee. Microscopy Research and Technique 45: 174-183.
  6. Callaerts-vegh, Z., T. Beckers, S.M. Ball, F. Baeyens, P.F. Callaerts, J.F. Cryan, E. Molnar & R. D'hooge, 2006. Concomitant deficits in working memory and fear extinction are functionally dissociated from reduced anxiety in metabotropic glutamate receptor 7-deficient mice. Journal of Neuroscience 26: 6573-6582.
  7. Chen, S.L., 2001. The Apicultural Science in China. China Agriculture Press,
  8. Beijing. Cline, H.T., G.Y. Wu & R. Malinow, 1996. In vivo development of neuronal structure and function. Cold Spring Harbor Symposia on Quantitative Biology 61: 95–104.
  9. Corona, M., R.A. Velarde, S. Remolina, A. Moran-lauter, Y. Wang, K.A. Hughes & G.E. Robinson, 2007. Vitellogenin, juvenile hormone, insulin signaling, and queen honeybee longevity. Proceedings of the National academy of Sciences of the United States of America 104: 7128–7133.
  10. Danbolt, N.C., 2001. Glutamate Uptake. Progress in Neurobiology 65: 1-105. Démares. F., F. Drouard, I. Massou, C. Crattelet, A. Loeuillet, C. Bettiol, & V. Raymond, 2014. Catherine armengaud differential involvement of glutamate-gated chloride channel splice variants in the olfactorymemory processes of the honeybee Apis mellifera. Pharmacology, Biochemistry and Behavior 124:137-144. Démares, F., V. Raymond & C. Armengaud, 2013. Expression and localization of glutamate-gated chloride channel variants in honeybee brain (Apis mellifera). Insect Biochemistry Molecule Biology 43(1):115-24.
  11. Denison, R.