Journals / Veterinary Journal of Mehmet Akif Ersoy University / 2017 / Cilt: 2 Sayı: 1

The Protective Effects of Egg Yolks of Different Poultry Species in Domestic Animal Semens Cryopreservation

Evcil Hayvan Spermalarının Dondurulmasında Farklı Kanatlı Türü Yumurta Sarılarının Koruyucu Etkileri

Pages
51–59
Publication Date
2017-09-13
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It has been concluded that egg yolks of different poultry species can be used as an alternative cryoprotectants instead of chicken egg yolk.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Until today, experimentally designed technical adjustments have been introduced for many types in semen freezing, which has become a requirement for many fields such as artificial insemination, species protection and clinical medicine.
📊
Key Findings & Data:Egg yolk, provided that it is added as an additive in many diluents used for freezing mammalian sperm; has taken place thanks to the protective effects of the contained special agents.
💡
Conclusion & Impact:It has been concluded that egg yolks of different poultry species can be used as an alternative cryoprotectants instead of chicken egg yolk.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:sperma dondurma · sulandırıcı · yumurta sarısı

Abstract

Until today, experimentally designed technical adjustments have been introduced for many types in semen freezing, which has become a requirement for many fields such as artificial insemination, species protection and clinical medicine. Egg yolk, provided that it is added as an additive in many diluents used for freezing mammalian sperm; has taken place thanks to the protective effects of the contained special agents. For this purpose, it widely used and has an effect on motility, viability and fertilization capacity. Protein, lipid and cholesterol present in the egg yolk are available to actively protect the sperm during various stages of freezing. In order to achieve better semen quality, the use of egg yolks of different poultry species instead of chicken egg yolks, which are traditionally used, has been tried and positive results have been obtained, albeit different according to the animal species. This condition may be due to the differences in biochemical content of egg yolks. These chemical differences can be explained by post-thaw motility and integrity differences in semen freezing process with extenders, which produced from egg yolks of different poultry species. The positive results were obtained in freezing of stallion, buffalo, bull, ram and boar semen, using of duck, goose, quail, pigeon, turkey and partridge egg yolks instead of chicken egg yolk. It has been concluded that egg yolks of different poultry species can be used as an alternative cryoprotectants instead of chicken egg yolk. However, this assessment needs to be retried for dose planning to become more clear.

Özet

Günümüze kadar suni tohumlama, tür koruma ve klinik tıp gibi birçok alanda gereksinim haline gelen sperma dondurulmasında, birçok tür için deneysel olarak tasarlanmış teknik ayarlamalar kullanıma girmiştir. Memeli spermalarının dondurulması için kullanılan birçok sulandırıcı içerisinde katkı maddesi olarak ilave edilmesi koşuluyla yumurta sarısı; içerdiği özel ajanların koruyucu etkileri sayesinde yerini almıştır. Bu amaçla geniş çapta kullanılmakta ve spermatozoon motilitesi, canlılığı ve fertilizasyon kapasitesi üzerinde etkiye sahiptir. Yumurta sarısında bulunan protein, lipit ve kolesterol dondurma sürecinde çeşitli aşamalar boyunca spermayı aktif olarak korumak için mevcutturlar. Daha iyi çözüm sonu sperma kalitesi elde etmek amacıyla farklı kanatlı türlerinin yumurta sarılarının geleneksel olarak kullanılan tavuk yumurta sarısı yerine kullanımı denenmiş ve hayvan türlerine göre farklı olmakla birlikte olumlu sonuçlar alınmıştır. Bu durum yumurta sarılarının biyokimyasal içeriklerinin farklılığından kaynaklanabilir. Bu kimyasal farklılıklar; farklı kanatlı türlerinin yumurta sarılarının oluşturduğu sulandırıcılarla yapılan sperma dondurulması işlemindeki çözüm sonu motilitesi ve spermatozoon bütünlüğü farklılıklarıyla açıklanabilir. Aygır, manda, boğa, koç ve domuz spermalarının dondurulmasında tavuk yumurta sarısı yerine kullanılan ördek, kaz, bıldırcın, güvercin, hindi ve keklik yumurta sarıları ile olumlu sonuçlar elde edilmiştir. Farklı kanatlı türlerinin yumurta sarılarının, tavuk yumurta sarısı yerine alternatif bir kriyoprotektan olarak kullanılabileceği fakat bu değerlendirmenin daha net olabilmesi için doz planlamalarının ayrı ayrı denenmesi gerektiği sonucuna varılmıştır.

🔖

Cite This Article

YALÇIN, B., AKAL, E. (2017). The Protective Effects of Egg Yolks of Different Poultry Species in Domestic Animal Semens Cryopreservation. Veterinary Journal of Mehmet Akif Ersoy University, Cilt: 2 Sayı: 1, 51–59. https://ajindex.com/articles/931290

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (20)

  1. Holt WV, 2000: Basic aspects of frozen storage of semen. Anim Reprod Sci, 62, 3–22
  2. Phillips PH, Lardy HA, 1940: A yolk-buffer pablum for the preservation of bull semen. J Dairy Sci, 23, 399-404
  3. Watson P, Martin I, 1975: Effects of egg yolk, glycerol and the freezing rate on the viability and acrosomal structures of frozen ram spermatozoa. Aust J Biol Sci, 28, 153-159
  4. Johnson LA, Weitze KF, Fiser P, Maxwell WM, 2000: Storage of boar semen. Anim Reprod Sci, 62, 143-172
  5. Salamon S, Maxwell WM, 2000: Storage of ram semen. Anim Reprod Sci, 62, 77–111
  6. Barbas JP, Mascarenhas RD, 2009: Cryopreservation of domestic animal sperm cells. Cell Tissue Bank, 10, 49–62
  7. Milovanov VK, Sokolovskaja II, 1980: The results and perspectives of scientific research. Zhivotnovodstvo, 10, 48–50
  8. Purdy PH, 2006: A review on goat sperm cryopreservation. Small Rum Res, 6, 215–225
  9. Medeiros CM, Forell F, Oliveira AT, Rodrigues JL, 2002: Current status of sperm cryopreservation: why isn’t better. Theriogenology, 57, 327–344
  10. Bathgate R, Maxwell WMC, Evans G, 2006: Studies on the effect of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality. Reprod Dom Anim, 41, 68–73
  11. Cross NL, 1998: Role of cholesterol in sperm capacitation. Biol Reprod, 59, 7–11
  12. Bailey JL, Bilodeau JF, Cormier N, 2000: Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon. J Androl, 21, 1-7
  13. Agarwal A, Prabakaran SA, Said TM, 2005: Prevention of oxidative stress injury to sperm. J Androl, 26, 654-660
  14. Gholami M, Faraji Z, Zamiri MJ, 2012: Effect of egg yolk of four avian species on the cryopreserved ram spermatozoa. Iran J Agric Res, 13(1), 23-27
  15. Phillips PH, 1939: Preservation of bull semen. J Biol Chem, 130, 415
  16. Pace MM, Graham EF, 1974: Components in egg yolk which protect bovine spermatozoa during freezing. J Anim Sci, 39, 1144–1149
  17. Blackshaw AW, 1954: The prevention of temperature shock of bull and ram semen. J Biol Sci, 7, 573-582
  18. Madden TD, Chapman D, Quinn PJ, 1979: Cholesterol modulates activity of calcium-dependent ATPase of the sarcoplasmic reticulum. Nature Lond, 279, 538-541
  19. Quinn PJ, White IG, Cleland KW, 1969: Chemical and ultrastructural changes in ram spermatozoa after washing, cold shock and freezing. J Reprod Fert, 18, 209-220
  20. Trimeche A, Anton M, Renard P, Gandemer G, Tainturier D, 1997: Quail egg yolk: a novel cryoprotecta