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Journals / Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi / 2023 / Cilt: 23 Sayı: 2

Phase-Contrast Imaging at Optical Microscope with a Light Emitting Diode Array

Işık Yayan Diyot Dizisi ile Işık Mikroskobunda Faz-Kontrast Görüntüleme

Nur Efşan KÖKSAL
İlyas ÇANKAYA
Esra ŞENGÜN ERMEYDAN
Pages
523–531
Publication Date
2023-05-03
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, the system used is simple, low-cost and compatible with the conventional light microscopy.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, a single-camera imaging system that can simultaneously acquire bright-field, dark-field and differential phase-contrast images is used with different illumination patterns through a programmable light-emitting diode (LED) array.
📊
Key Findings & Data:Bright-field, dark-field and differential phasecontrast images are obtained without any moving parts by changing the patterns of 32x32 LED array.
💡
Conclusion & Impact:Therefore, the system used is simple, low-cost and compatible with the conventional light microscopy.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Işık mikroskobu · LED dizisi · Faz-kontrast görüntüleme

Abstract

In this study, a single-camera imaging system that can simultaneously acquire bright-field, dark-field and differential phase-contrast images is used with different illumination patterns through a programmable light-emitting diode (LED) array. The LED array allows flexible modeling of illumination angles. The effect of left-right, top-bottom and oblique illumination was observed in detail both in the cheek epithelial cell and in the onion membrane cell. Bright-field, dark-field and differential phasecontrast images are obtained without any moving parts by changing the patterns of 32x32 LED array. Therefore, the system used is simple, low-cost and compatible with the conventional light microscopy.

Özet

Bu çalışmada, aydınlık-alan, karanlık-alan ve diferansiyel faz-kontrast görüntülerini aynı anda elde edebilen tek-kameralı bir görüntüleme sistemi, programlanabilir bir ışık yayan diyot (LED) dizisi aracılığıyla farklı aydınlatma modelleri ile birlikte kullanılmıştır. Kullanılan LED dizisi, aydınlatma açılarının esnek bir şekilde modellenmesine olanak sağlamıştır. Sol-sağ, üst-alt ve eğik aydınlatmaların etkisi, hem yanak epitel hücresinde hem de soğan zarı hücresinde detaylı olarak gözlemlenmiştir. Aydınlık-alan, karanlık-alan ve diferansiyel faz-kontrast görüntüleri, 32x32 LED dizisinin desenleri değiştirilerek herhangi bir hareketli parça olmadan elde edilmiştir. Bu nedenle, kullanılan sistem basittir, düşük maliyetlidir ve geleneksel ışık mikroskobu ile uyumludur.

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Cite This Article

KÖKSAL, N. E., ÇANKAYA, İ., ERMEYDAN, E. Ş. (2023). Phase-Contrast Imaging at Optical Microscope with a Light Emitting Diode Array. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 23 Sayı: 2, 523–531. https://ajindex.com/articles/920851

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