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Journals / Veterinary Sciences and Practices / 2019 / Cilt: 14 Sayı: 3

Changes in Sperm Parameters Following Administration of Theophylline, a Competitive Antagonist of Adenosine in Rats Exposed to Bleomycin, a Chemotherapeutic Agent

Bir Kemoterapotik Ajan Olan Bleomisine Maruz Kalan Ratlarda Adenozinin Kompetetif Antagonisti Olan Teofilinin Uygulanması Sonrasında Sperm Parametrelerindeki Değişimler

Saadet Belhan(Yuzuncu Yıl University, Veterinary Faculty, Department of Reproduction and Artificial Insemination, TR-65090 VAN – TURKEY.)0000-0002-8115-2051
Gökhan Oto(Yuzuncu Yıl University, Faculty of Medicine, Department of Pharmacology, TR-65090 VAN – TURKEY.)0000-0001-7310-7800
Okan Arıhan(Hacettepe University, Faculty of Medicine, Department of Physiology, TR-06800 ANKARA – TURKEY.)0000-0001-6201-7383
Volkan Koşal(Yuzuncu Yıl University, Veterinary Faculty, Department of Reproduction and Artificial Insemination, TR-65090 VAN – TURKEY.)0000-0001-5268-5046
Pages
246–251
Publication Date
2019-12-25
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

There was no positive effect on sperm parameters except motility.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of the present study is to determine the effects of theophylline (THP), a methylxanthine derivative, on the sperm parameters of rats exposed to bleomycin (BLM) as a chemotherapeutic agent.
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Key Findings & Data:The study was performed on 32 male rats.
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Conclusion & Impact:There was no positive effect on sperm parameters except motility.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Bleomisin · Rat · Sperm Parametreleri

Abstract

The aim of the present study is to determine the effects of theophylline (THP), a methylxanthine derivative, on the sperm parameters of rats exposed to bleomycin (BLM) as a chemotherapeutic agent. The study was performed on 32 male rats. The rats in Group 1 were treated only with sterile saline intratracheally. The rats in Group 2 were administered a single dose of 7.5 mg/kg of BLM intratracheally. The rats in Group 3 received a single dose of 7.5 mg/kg of BLM intratracheally. In addition, THP was administered to rats in Group 3 intraperitoneally at a dose of 75 mg/kg/ day for 14 days. The rats in group 4 were administered an intraperitoneal injection of THP at a dose of 75 mg/kg/day for 14 days. Motility, sperm density, abnormal sperm and dead sperm rates were examined. Significant deteriorations were observed in sperm parameters of rats that were administered BLM. In the sperm parameters of THP treated rats, it was determined that it only increased motility, but did not significantly change sperm density, abnormal sperm and dead sperm count. In this study, THP only increased motility. There was no positive effect on sperm parameters except motility.

Özet

Bu çalışmanın yapılmasındaki amaç, bir kemoterapotik ajan olan bleomisine (BLM) maruz bırakılan ratlarda metilksantin türevi olan teofilinin (THP) sperm parametreleri üzerinde ne gibi etkiler göstereceğini belirlemektir. Çalışma 32 adet erkek rat üzerinde yapıldı. Grup 1’deki ratlara sadece steril serum fizyolojik intratrakeal yoldan uygulandı. Grup 2’deki ratlara, 7.5 mg/kg BLM intratrakeal olarak tek doz uygulandı. Grup 3’deki ratlara, 7.5 mg/kg BLM intratrakeal olarak tek doz uygulandı. İlave olarak 14 gün boyunca 75 mg/kg/gün dozunda THP intraperitoneal yoldan uygulandı. Grup 4’deki ratlara, 14 gün boyunca 75 mg/kg/gün dozunda THP intraperitoneal yoldan uygulandı. Motilite, sperm yoğunluğu, anormal sperm ve ölü sperm oranlarına bakıldı. BLM uygulanan ratların sperm parametrelerinde ciddi bozulmalar tespit edildi. THP uygulanan ratların sperm parametrelerinde sadece motilitede anlamlı artışlar oluşturduğu, ancak sperm yoğunluğu, anormal sperm ve ölü sperm sayısı üzerinde anlamlı olarak değişiklikler yapmadığı tespit edildi. Bu çalışmada THP sadece motiliteyi arttırmıştır. Motilite dışında sperm parametreleri üzerinde olumlu bir etkisi yoktu.

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Cite This Article

Belhan, S., Oto, G., Arıhan, O., Koşal, V. (2019). Changes in Sperm Parameters Following Administration of Theophylline, a Competitive Antagonist of Adenosine in Rats Exposed to Bleomycin, a Chemotherapeutic Agent. Veterinary Sciences and Practices, Cilt: 14 Sayı: 3, 246–251. https://doi.org/10.17094/ataunivbd.563809

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