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Journals / Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji / 2019 / Cilt: 8 Sayı: 2

FUNCTIONAL INTERACTION BETWEEN caf5 AND SPERMIDINE FAMILY TRANSPORTERS IN S. POMBE

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Aslıhan ÖRS GEVREKCİ
Pages
170–178
Publication Date
2019-07-31
DOI
—
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The results showed that the double mutants differentiate from each other in their combined mutation with caf5 in terms of cell size and DNA damage response, indicating redundant seperate pathways of spermine and spermidine transport in S. pombe.

📌 3 Key Takeaways

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Objective & Hypothesis:ABSTRACT Polyamines are well known for their wide range of roles in the cells, including stabilization of nucleic acids, regulation of gene expression at different levels, stress response and cell cycle.
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Key Findings & Data:Previously, two spermine family transporters SPBC409.08 and caf5 were characterized which were associated with cell size defects.
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Conclusion & Impact:The results showed that the double mutants differentiate from each other in their combined mutation with caf5 in terms of cell size and DNA damage response, indicating redundant seperate pathways of spermine and spermidine transport in S. pombe.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:S. pombe · Cell Size · Cell Cycle

Abstract

ABSTRACT Polyamines are well known for their wide range of roles in the cells, including stabilization of nucleic acids, regulation of gene expression at different levels, stress response and cell cycle. The activity of these well conserved small polycations are also regulated by different mechanisms to assure their intracellular concentration and activity. Polyamine transporters have a major role in the regulation of the influx and efflux of the polyamines. Previously, two spermine family transporters SPBC409.08 and caf5 were characterized which were associated with cell size defects. The aim of this study is to better understand the role of caf5 plasma membrane spermine transporter and its functional interaction with spermidine transporters. Double mutants of caf5 and spermidine family transporters SPBC36.01c, SPBC36.03c and SPBC569.05c were formed in S. pombe. The double mutants were then put through growth rate analysis, meiotic progression and sporulation analysis, cell cycle analysis, and vulnerability to environmental stress. The results showed that the double mutants differentiate from each other in their combined mutation with caf5 in terms of cell size and DNA damage response, indicating redundant seperate pathways of spermine and spermidine transport in S. pombe.

Özet

Polyamines are well known for their wide range of roles in the cells, including stabilization of nucleic acids, regulation of gene expression at different levels, stress response and cell cycle. The activity of these well conserved small polycations are also regulated by different mechanisms to assure their intracellular concentration and activity. Polyamine transporters have a major role in the regulation of the influx and efflux of the polyamines. Previously, two spermine family transporters SPBC409.08 and caf5 were characterized which were associated with cell size defects. The aim of this study is to better understand the role of caf5 plasma membrane spermine transporter and its functional interaction with spermidine transporters. Double mutants of caf5 and spermidine family transporters SPBC36.01c, SPBC36.03c and SPBC569.05c were formed in S. pombe. The double mutants were then put through growth rate analysis, meiotic progression and sporulation analysis, cell cycle analysis, and vulnerability to environmental stress. The results showed that the double mutants differentiate from each other in their combined mutation with caf5 in terms of cell size and DNA damage response, indicating redundant seperate pathways of spermine and spermidine transport in S. pombe.

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Cite This Article

GEVREKCİ, A. Ö. (2019). FUNCTIONAL INTERACTION BETWEEN caf5 AND SPERMIDINE FAMILY TRANSPORTERS IN S. POMBE. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, Cilt: 8 Sayı: 2, 170–178. https://ajindex.com/articles/905315

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