Journals / Tarım Bilimleri Dergisi / 2023 / Cilt: 29 - Sayı: 1

Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR

Pages
287–297
Publication Date
2023-01-31
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

No S-alleles were observed in 2 genotypes (Pancarlık and Hüryemez) which exhibited ‘Compatibility’ status.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented.
📊
Key Findings & Data:Self-incompatibility results in decreased pollination and ultimately yield loss.
💡
Conclusion & Impact:No S-alleles were observed in 2 genotypes (Pancarlık and Hüryemez) which exhibited ‘Compatibility’ status.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Malus domestica L. · Self-incompatibility · Anatolian gen resourses

Abstract

Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple (Malus domestica L.), self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes (such as, Amasya, Hüryemez, Şah elması, Tokat, Demir elması etc.) obtained from the National Collection of Atatürk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR (Polymerase Chain Reaction) using 4 different S-alleles (Sd, Sf, S26 and S9). 181 genotypes containing at least 1 S-allele were identified as ‘Partially Incompatible’ and 12 genotypes involving 4 S-alleles were assigned ‘Totally Incompatible’. No S-alleles were observed in 2 genotypes (Pancarlık and Hüryemez) which exhibited ‘Compatibility’ status.

🔖

Cite This Article

KARATAŞ, M. D., HAZRATİ, N., ÖZMEN, C. Y., HASANZADEH, M., ALTINTAŞ, S., AKÇAY, M. E., ERGÜL, A. (2023). Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR. Tarım Bilimleri Dergisi, Cilt: 29 - Sayı: 1, 287–297. https://ajindex.com/articles/880200

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Abdallah D, Baraket G, Perez V, Mustapha S B, Salhi-Hannachi A & Hormaza J I (2019). Analysis of self-incompatibility and genetic diversity in diploid and hexaploid plum genotypes. Frontiers in Plant Science 10:896. https://dx.doi.org/10.3389/fpls.2019.00896
  2. Brancher T L, Hawerroth M C, Kvitschal M V, Manenti D C & Guidolin A F (2020). Self-incompatibility alleles in important genotypes for apple breeding in Brazil. Crop Breeding and Applied Biotechnology 20 (4):e28652041. https://doi.org/10.1590/1984-70332020v20n4a54 Broothaerts W & Van Nerum I (2003). Apple self-incompatibility genotypes: an overview. In XXVI International Horticultural Congress: Genetics and Breeding of Tree Fruits and Nuts 379-387,
  3. Canada. Broothaerts W, Janssens G A, Proost P & Broekaert W F (1995). Cdna cloning and molecular analysis of two self-incompatibility alleles from apple. Plant Molecular Biology 27 (3): 499-511. https://doi.org/10.1007/bf00019317
  4. Broothaerts W, Neram I V & Keulemans J (2004). Update on and Review of the Incompatibility (S-) Genotypes of Apple Cultivars. HortScience: a publication of the American Society for Horticultural Science 39(5):943-947. https://doi.org/10.21273/hortsci.39.5.943
  5. Broothaerts, W, Verdoodt, L, Keulemans, J, Janssens, G A & Broekaert, W F (1996). The self-incompatibility gene in apple and determination of the S-genotype of apple cultivars by PCR. Acta Horticulturae 423:103-109. https://doi.org/10.17660/ActaHortic.1996.423.13
  6. Burak M, Ergül A, Kazan K, Akçay M E, Yüksel C, Bakir M & Ayanoğlu H (2014). Genetic analysis of Anatolian apples (Malus sp.) by simple sequence repeats. Journal of Systematics and Evolution 52(5):580-588. https://doi.org/10.1111/jse.12099
  7. De Franceschi P, Bianco L, Cestaro A, Dondini L & Velasco R (2018). Characterization of 25 full-length S-RNase alleles, including flanking regions, from a pool of resequenced apple cultivars. Plant Molecular Biology 97:279-296. https://doi.org/10.1007/s11103-018-0741-x
  8. De Franceschi P, Cova V, Tartarini S & Dondini L (2016). Characterization of a new apple S-RNase allele and its linkage with the Rvi5 gene for scab resistance. Molecular Breeding 36:1-11. https://doi.org/10.1007/s11032-015-0427-x
  9. De Franceschi P, Dondini L & Sanzol J (2012) Molecular bases and evolutionary dynamics of self-incompatibility in the Pyrinae (Rosaceae). Journal of Experimental Botany 63(11): 4015-4032. https://doi.org/10.1093/jxb/ers108
  10. De Nettancourt D (2001). Incompatibility and Incongruity in Wild and Cu