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Journals / Journal of Agricultural Sciences / 2026 / Volume: 29 Issue: 1

Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR

Merve Dilek Karataş(VAN YUZUNCU YIL UNIVERSITY, FACULTY OF AGRICULTURE, AGRICULTURAL BIOTECHNOLOGY PR.)0000-0002-1076-3648
Nahid Hazrati(ANKARA UNIVERSITY, INSTITUTE OF BIOTECHNOLOGY)0000-0002-8696-1480
Canan Yüksel Özmen(ANKARA UNIVERSITY, INSTITUTE OF BIOTECHNOLOGY)0000-0002-4421-1358
Mohammad Hasanzadeh(Mohaghegh Ardabili University, Faculty of Agriculture and Natural Resources-Moghan, Department of Plant Production)0000-0002-6379-4632
Serdar Altıntaş(SIIRT UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF AGRICULTURAL BIOTECHNOLOGY)0000-0001-6324-5265
Mehmet Emin Akçay(Republic of Turkey Ministry of Agriculture and Forestry, Atatürk Horticultural Central Research Institute)0000-0002-6992-782X
Ali Ergül(ANKARA UNIVERSITY BIOTECHNOLOGY INSTITUTE)0000-0002-1205-268X
Pages
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Publication Date
2023-01-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

No S-alleles were observed in 2 genotypes (Pancarlık and Hüryemez) which exhibited ‘Compatibility’ status.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented.
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Key Findings & Data:Self-incompatibility results in decreased pollination and ultimately yield loss.
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Conclusion & Impact:No S-alleles were observed in 2 genotypes (Pancarlık and Hüryemez) which exhibited ‘Compatibility’ status.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Malus domestica L. · Self-incompatibility · Anatolian gen resourses

Abstract

Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple (Malus domestica L.), self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes (such as, Amasya, Hüryemez, Şah elması, Tokat, Demir elması etc.) obtained from the National Collection of Atatürk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR (Polymerase Chain Reaction) using 4 different S-alleles (Sd, Sf, S26 and S9). 181 genotypes containing at least 1 S-allele were identified as ‘Partially Incompatible’ and 12 genotypes involving 4 S-alleles were assigned ‘Totally Incompatible’. No S-alleles were observed in 2 genotypes (Pancarlık and Hüryemez) which exhibited ‘Compatibility’ status.

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Cite This Article

Karataş, M. D., Hazrati, N., Özmen, C. Y., Hasanzadeh, M., Altıntaş, S., Akçay, M. E., Ergül, A. (2023). Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR. Journal of Agricultural Sciences, Volume: 29 Issue: 1. https://doi.org/10.15832/ankutbd.930238

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