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Journals / Marmara Medical Journal / 2023 / Cilt: 36 - Sayı: 1

Molecular analysis of human adenoviral keratoconjunctivitis cases: Results of a 2-year survey

Ayfer GUNER
Rabia CAN SARINOGLU
Fahri Onur AYDIN
Semra AKKAYA TURHAN
Mert Ahmet KUSKUCU
Ayse Ebru TOKER
Aysegul KARAHASAN YAGCI
Pages
1–6
Publication Date
2023-01-31
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

The clinical finding score was found to be significantly higher in the high viral load group (Adenovirus DNA≥6 Log 10 copies/mL) than in the low viral load group (Adenovirus DNA

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: This study aimed to determine the adenovirus genotypes and their epidemiological features between January 2018 and November 2019, in Istanbul, Turkey.
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Key Findings & Data:For subtyping of the strains, sequencing primers targeted the ‘Hypervariable Region 7’ (HVR-7) of the hexon gene were used.
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Conclusion & Impact:The clinical finding score was found to be significantly higher in the high viral load group (Adenovirus DNA≥6 Log 10 copies/mL) than in the low viral load group (Adenovirus DNA
🔬Design:Research Article
📍Scope:2018 Verileri
⚙️Variables:Adenovirus · Genotyping · Viral conjunctivitis

Abstract

Objective: This study aimed to determine the adenovirus genotypes and their epidemiological features between January 2018 and November 2019, in Istanbul, Turkey. Material and Methods: Conjunctival swab samples were obtained from patients who were clinically diagnosed with keratoconjunctivitis. Samples were screened with an Adeno Detector kit (Rapid Pathogen Screening, RPS Inc., South Williamsport, PA). Nucleic acid extraction and amplification were performed with the ADENOVIRUS ELITe MGB® kit in the ELITe In Genius instrument (Elitech Group, Torino, Italy). For subtyping of the strains, sequencing primers targeted the ‘Hypervariable Region 7’ (HVR-7) of the hexon gene were used. DNA sequence analysis (n:72) was performed with ABI PRISM® 3100 Genetic Analyzer (Applied Biosystems, USA), and subtyping was done by BLAST analysis. Results: The median viral load in the samples (n: 77) was 7 log10 copies/mL (IQR: 4.5-7.4 log10 copies/mL). The clinical finding score was found to be significantly higher in the high viral load group (Adenovirus DNA≥6 Log 10 copies/mL) than in the low viral load group (Adenovirus DNA

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Cite This Article

GUNER, A., SARINOGLU, R. C., AYDIN, F. O., TURHAN, S. A., KUSKUCU, M. A., TOKER, A. E., YAGCI, A. K. (2023). Molecular analysis of human adenoviral keratoconjunctivitis cases: Results of a 2-year survey. Marmara Medical Journal, Cilt: 36 - Sayı: 1, 1–6. https://ajindex.com/articles/879979

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References (6)

  1. Shenk T. Adenoviridae: the viruses and their replication. In Fields Virology. Philadelphia, PA: Lippincott Williams & Wilkins Co., 1996: 2111, 2148. Berk AJ. Adenoviridae. In: Knipe DM, Howley PM, eds. Fields Virology vol. 2, 6th ed. Philadelphia: Lippincott Williams and Wilkins, 2013:1704, 1731. Robinson CM, Singh G, Lee JY, et al. Molecular evolution of human adenoviruses. Sci Rep 2013; 3:1812. doi: 10.1038/ srep01812. Dhingra A, Hage E, Ganzenmueller T, et al. Molecular evolution of human adenovirus (HAdV) species C. Sci Rep 2019; 9:1039. doi:10.1038/s41598.018.37249-4
  2. Jhanji V, Chan TC, Li EY, Agarwal K, Vajpayee RB. Adenoviral keratoconjunctivitis. Surv Ophthalmol 2015; 60: 435-43. doi: 10.1016/j.survophthal.2015.04.001. Lenaerts L, De Clercq E, Naesens L. Clinical features and treatment of adenovirus infections. Rev Med Virol 2018; 18:357-74. doi: 10.1002/rmv.589. PMID: 18655013 Gordon YJ, Gordon RY, E. Romanowski E, Araullo-Cruz TP. Prolonged recovery of desiccated adenoviral serotypes 5, 8, and 19 from plastic and metal surfaces in vitro. Ophthalmology 1993; 100:1835-1840. doi: 10.1016/s0161-6420(93)31389-8.
  3. Cheung D, Bremner J, Chan JT. Epidemic keratoconjunctivitis— Do outbreaks have to be epidemic? Eye 2003; 17:356-63. doi: 10.1038/sj.eye.6700330. Melendez CP, Florentino MM, Martinez IL, Lopez HM. Outbreak of epidemic keratoconjunctivitis caused by adenovirus in medical residents. Mol Vis 2009; 15:557-562.
  4. Binder AM, Biggs HM, Haynes AK, et al. Human adenovirus surveillance – United States, 2003-2016. MMWR Morb Mortal Wkly Rep 2017; 66(39):1039-42. doi: 10.15585/mmwr. mm6639a2 Siamak NM, Kowalski RP, Thompson PP, Romanowski EG, Shanks RM, Gordon YJ. RPS adeno detector. Ophthalmology 2009; 116:591-1.e1. doi: 10.1016/j.ophtha.2008.10.025. Sarantis H, Johnson G, Brown M, Petric M, Tellier R. Comprehensive detection and serotyping of human adenoviruses by PCR and sequencing. J Clin Microbiol 2014; 42:3963-9. doi: 10.1128/JCM.42.9.3963-3969.2004.
  5. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403-10. doi: 10.1016/S0022-2836(05)80360-2.
  6. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9. doi: 10.1093/molbev/msr121. Banik U, Adhikary AK, Suzuki E, Inada T, Okabe N. Multiplex PCR assay for rapid identification of oculopathogenic