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Journals / Sağlık Bilimlerinde Değer / 2023 / Cilt: 13 - Sayı: 1

Cytotoxic Effects of Borax via GPx4/ACSL4 Signaling Pathway in Colorectal Cancer Cells

Kolorektal Kanser Hücrelerinde Boraksın Gpx4/ACSL4 Sinyal Yolu Aracılığıyla Sitotoksik Etkileri

Ceyhan HACIOĞLU
Fatih DAVRAN
Pages
54–60
Publication Date
2023-01-20
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: Borax at 1 and 4 mM concentrations did not affect cell viability, while borax at 8 mM and above concentrations significantly reduced viability in HTC-116 cells (p

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Colorectal cancer (CRC) ranks third in terms of malignancy and second in cancer-related deaths, accounting for approximately 10% of cancer-related deaths.
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Key Findings & Data:Ferroptosis is a potential iron-induced cell death pathway that could increase the anti-cancer efficacy of therapeutics in preventing drug resistance in the treatment of many cancers, including CRC.
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Conclusion & Impact:Results: Borax at 1 and 4 mM concentrations did not affect cell viability, while borax at 8 mM and above concentrations significantly reduced viability in HTC-116 cells (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Boraks · DNA hasarı · ferroptoz

Abstract

Aim: Colorectal cancer (CRC) ranks third in terms of malignancy and second in cancer-related deaths, accounting for approximately 10% of cancer-related deaths. Ferroptosis is a potential iron-induced cell death pathway that could increase the anti-cancer efficacy of therapeutics in preventing drug resistance in the treatment of many cancers, including CRC. In this study, we aimed to investigate the anti-proliferative effects of borax via ferroptosis signaling pathway in CRC cells. Material and Methods: Firstly, we determined the cytotoxic concentrations of borax (range 0 to 64 mM) with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Subsequently, glutathione peroxidase 4 (GPx4), acyl-CoA synthetase long chain family member 4 (ACSL4), malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) levels were determined in HCT-116 cells incubated with cytotoxic borax concentrations for 24 hours. Results: Borax at 1 and 4 mM concentrations did not affect cell viability, while borax at 8 mM and above concentrations significantly reduced viability in HTC-116 cells (p

Özet

Amaç: Kolorektal kanser (CRC), kansere bağlı ölümlerin yaklaşık %10'unu oluşturmasıyla, malignite yönünden üçüncü ve kansere bağlı ölümlerde ikinci sırada yer almaktadır. Ferroptoz, CRC dahil çok sayıda kanserin tedavisinde ilaç direncini önlemede terapötiklerin anti-kanser etkinliğini arttırabilecek potansiyel demire bağlı hücre ölüm yolağıdır. Bu çalışmada CRC hücrelerinde ferroptozu sinyal yolağı üzerinden boraksın anti-proliferatif etkilerini araştırmak amaçlandı. Gereç ve Yöntemler: Öncelikle, boraksın sitotoksik konsantrasyonları (0-64 mM aralığında) 3-(4,5-dimetiltiazol-2-il)-2,5-difenil tetrazolyum bromür (MTT) testi ile belirlendi. Daha sonra sitotoksik boraks konsantrasyonları ile 24 saat inkübe edilen HCT-116 hücrelerinde glutatyon peroksidaz 4 (GPx4), açil-KoA sentetaz uzun zincirli aile üyesi 4 (ACSL4), malondialdehit (MDA) ve 8-hidroksideoksiguanozin (8-OHdG) seviyeleri belirlendi. Bulgular: 1 ve 4 mM boraks konsantrasyonları hücre canlılığını etkilemezken, 8 mM ve üzerindeki boraks konsantrasyonları HTC-116 hücrelerinde canlılığı anlamlı şekilde düşürmüştür (p

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Cite This Article

HACIOĞLU, C., DAVRAN, F. (2023). Cytotoxic Effects of Borax via GPx4/ACSL4 Signaling Pathway in Colorectal Cancer Cells. Sağlık Bilimlerinde Değer, Cilt: 13 - Sayı: 1, 54–60. https://ajindex.com/articles/877982

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