Journals / Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi / 2022 / Cilt: 9 - Sayı: 1

Anti-Proliferative Effect of Cytochalasin B Treatment on Nrf2 Signaling Pathway in U87 Glioblastoma Cells

U87 Glioblastoma Hücrelerinde Sitokalasin B Uygulamasının Nrf2 Sinyal Yolundaki Anti-Proliferatif Etkisi

Pages
92–98
Publication Date
2022-10-12
DOI
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Subsequently, TOS, MDA, and 8-OHdG levels increased in a concentration-dependent manner in U87 cells treated with 5.1, 33.6, and 62.8μM CB (p

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Objective & Hypothesis:Objective: Glioblastoma is one of the aggressive brain tumors with a poor prognosis, and the available treatments for glioblastoma are quite limited.
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Key Findings & Data:In this study, we aimed to investigate the effect of CB on oxidative, antioxidant and DNA damage mechanisms via nuclear factor erythroid 2 associated factor (Nrf2) signaling pathway in U87 human glioblastoma cells.
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Conclusion & Impact:Subsequently, TOS, MDA, and 8-OHdG levels increased in a concentration-dependent manner in U87 cells treated with 5.1, 33.6, and 62.8μM CB (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Glioblastoma is one of the aggressive brain tumors with a poor prognosis, and the available treatments for glioblastoma are quite limited. Cytochalasin B (CB) has an inhibitory effect on cancer cell lines, but its anticancer effects are not yet fully understood. In this study, we aimed to investigate the effect of CB on oxidative, antioxidant and DNA damage mechanisms via nuclear factor erythroid 2 associated factor (Nrf2) signaling pathway in U87 human glioblastoma cells. Materials and Methods: Firstly, we determined the cytotoxic concentrations of CB in U87 cells by MTT analysis. Then, the effect of CB on Nrf2 levels and related total oxidant capacity(TOS), malondialdehyde (MDA), superoxide dismutase(SOD) and glutathione peroxidase(GPx) levels were measured. Finally, 8-hydroxy-2'-deoxyguanosine(8- OHdG) levels were measured to detect DNA damage in U87 cells treated with CB. Results: According to MTT analysis, we determined that CB decreased viability in U87 cells in a concentrationdependent manner and determined the IC50 concentration to be 62.8μM. Subsequently, TOS, MDA, and 8-OHdG levels increased in a concentration-dependent manner in U87 cells treated with 5.1, 33.6, and 62.8μM CB (p

Özet

Giriş ve Amaç: Glioblastoma kötü prognozlu agrasif beyin tümörlerinden biridir ve glioblastoma için mevcut tedavi yöntemleri oldukça sınırlıdır. Sitokalasin B'nin (CB) kanser hücre hatları üzerinde inhibitör etki göstermektedir, ancak antikanser etkileri henüz tam olarak anlaşılamamıştır. Bu çalışmada, CB'nin U87 insan glioblastoma hücrelerinde nükleer faktör eritroid 2 ile ilişkili faktör (Nrf2) sinyal yolağı üzerinden oksidatif, antioksidan ve DNA hasar mekanizmaları üzerindeki etkisini araştırmayı hedefledik. Gereç ve Yöntemler: İlk olarak, CB'nin U87 hücrelerindeki sitotoksik konsantrasyonlarını MTT analizi ile belirledik. Ardından, CB'nin Nrf2 seviyeleri üzerindeki etkisini ve bununla bağlantılı olarak total oksidan kapasite (TOS), malondialdehit (MDA), süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GPx) seviyeleri ölçüldü. Son olarak, CB ile tedavi edilen U87 hücrelerindeki DNA hasarını tespit edebilmek için 8-hidroksi-2'-deoksiguanozin (8-OHdG) seviyeleri ölçüldü. Bulgular: MTT analizine göre, CB'nin U87 hücrelerinde canlılığı konsantrasyona bağımlı bir şekilde azalttığını belirledik ve IC50 konsantrasyonunu 62,8 μM olarak tespit ettik. Ardından, 5,1, 33,6 ve 62,8 μM CB ile tedavi edilen U87 hücrelerinde TOS, MDA ve 8-OHdG seviyeleri konsantrasyon bağımlı bir şekilde artmıştır (p

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Cite This Article

HACIOĞLU, C., KAR, F. (2022). Anti-Proliferative Effect of Cytochalasin B Treatment on Nrf2 Signaling Pathway in U87 Glioblastoma Cells. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, Cilt: 9 - Sayı: 1, 92–98. https://ajindex.com/articles/472616

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