Development and validation of an HPLC method for determination of quercetin

Objective & Hypothesis:Abstract This study validated a high performance liquid chromatography (HPLC) method for the determination of quercetin (QE) in microemulsion (ME) based gel formulations.

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Key Findings & Data:The analyses were performed on a C18 column (150x4.6 mm, 5 m particle size) at room temperature with UV detection at 254 nm.

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Conclusion & Impact:The method reported is a fast and reliable HPLC method useful for QE determination in ME based gel formulations.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Quercetin · microemulsion based gel · validation

Abstract

Abstract This study validated a high performance liquid chromatography (HPLC) method for the determination of quercetin (QE) in microemulsion (ME) based gel formulations. The analyses were performed on a C18 column (150x4.6 mm, 5 m particle size) at room temperature with UV detection at 254 nm. The mobile phase was composed of methanol:water (65:35, v/v, 2% acetic acid) mixture, and flow rate was set to 1 mL/min. The method was validated according to the international guidelines with respect to linearity range, stability, limit of quantitation and detection, precision and accuracy. The method was linear within 5 – 100 µg/mL range with a correlation coefficient of 0.9995. The intra- and inter-assay precisions presented RSD values lower than 2%. The method reported is a fast and reliable HPLC method useful for QE determination in ME based gel formulations.

Keywords

Quercetin microemulsion based gel validation HPLC

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Cite This Article

ONAN, D., ÇEVİKELLİ, T., GÜVEN, U. M., DEMİRTÜRK, E. (2022). Development and validation of an HPLC method for determination of quercetin. Journal of Pharmaceutical Technology, Cilt: 3 - Sayı: 1, 1–6. https://ajindex.com/articles/872826

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