Journals / Biotech Studies / 2022 / Cilt: 31 - Sayı: 2

In food safety control overview of using Real-Time PCR

Pages
53–60
Publication Date
2022-12-31
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The quality control analyzes in which the method was used were classified and explanations were made about each analysis area and examples from the studies in the literature were given.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The identity-determining importance of nucleic acids in living beings is a guide for reaching the desired information about food in quality control analyzes.
📊
Key Findings & Data:With these goals, the popularity of Real-time PCR (Polymerase Chain Reaction) methods, which are one of the nucleic acid-based methods, is rapidly increasing due to their high reproducibility, precision and fast result production.
💡
Conclusion & Impact:The quality control analyzes in which the method was used were classified and explanations were made about each analysis area and examples from the studies in the literature were given.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Real-Time PCR · Food quality · Adulteration

Abstract

The identity-determining importance of nucleic acids in living beings is a guide for reaching the desired information about food in quality control analyzes. With these goals, the popularity of Real-time PCR (Polymerase Chain Reaction) methods, which are one of the nucleic acid-based methods, is rapidly increasing due to their high reproducibility, precision and fast result production. It is thought that informative studies about the analysis using the device will provide a basis for researches on the subject. This review contains information about the studies conducted on Real-Time PCR analysis used to detect imitation / adulteration and cheating in foods. General descriptions about the operation of the Real-Time PCR methods are given. The quality control analyzes in which the method was used were classified and explanations were made about each analysis area and examples from the studies in the literature were given.

🔖

Cite This Article

ARTUVAN, E., AKSAY, S. (2022). In food safety control overview of using Real-Time PCR. Biotech Studies, Cilt: 31 - Sayı: 2, 53–60. https://ajindex.com/articles/858194

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Agrimonti, C., Bottari, B., Sardaro, M. L., & Marmiroli, N. (2019). Application of real-time PCR (qPCR) for characterization of microbial populations and type of milk in dairy food products. Critical Reviews in Food Science and Nutrition, 59(3), 423-442. https://doi.org/10.1080/10408398.2017.1375893
  2. Agrimonti, C., Pirondini, A., Marmiroli, M., & Marmiroli, N. (2015). A quadruplex PCR (qxPCR) assay for adulteration in dairy products. Food Chemistry, 187, 58-64. https://doi.org/10.1016/j.foodchem.2015.04.017
  3. Ahmed, F. E. (2002). Detection of genetically modified organisms in foods. Trends in Biotechnology, 20(5), 215-223. https://doi.org/10.1016/S0167-7799(01)01920-5
  4. Alary, R., Serin, A., Maury, D., Jouira, H. B., Sirven, J.-P., Gautier, M.-F., & Joudrier, P. (2002). Comparison of simplex and duplex real-time PCR for the quantification of GMO in maize and soybean. Food Control, 13, 235-244. https://doi.org/10.1016/S0956-7135(02)00015-4
  5. Ali, M. E., Hashim, U., Dhahi, S. T., Mustafa, S., Man, Y., & Latif, M. A. (2012). Analysis of Pork Adulteration in Commercial Burgers Targeting Porcine-Specific Mitochondrial Cytochrome B Gene by TaqMan Probe Real-Time Polymerase Chain Reaction. Food Analytical Methods, 5, 784-794. http://dx.doi.org/10.1007/s12161-011-9311-4
  6. Alikord, M., Momtaz, H., Keramat, J., Kadivar, M., & Rad, A. H. (2018). Species identification and animal authentication in meat products: a review. Journal of Food Measurement and Characterization, 12, 145-155. https://doi.org/10.1007/s11694-017-9625-z
  7. Bottero, M., Civera, T., Nucera, D., Rosati, P., Sacchi, P., & Turi, R. (2003). A multiplex polymerase chain reaction for the identification of cows', goats' and sheep's milk in dairy products. International Dairy Journal, 13, 277-282. http://dx.doi.org/10.1016/S0958-6946(02)00170-X
  8. Böhme, K., Calo-Mata, P., Barros-Velazquez, J., & Ortea, I. (2019). Review of Recent DNA-Based Methods for Main Food-Authentication Topics. Journal of Agricultural and Food Chemistry, 67, 3854-3864. https://doi.org/10.1021/acs.jafc.8b07016
  9. Corman, V. M., Landt, O., Kaiser, M., Molenkamp, R., Meijer, A., Chu, D. K., Drosten, C. (2020). Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Eurosurveillance, 25(3), 1-8. https://dx.doi.org/10.2807%2F1560-7917.ES.2020.25.3.2000045
  10. Deniz, H. İ., & Kılıç Altun, S. (2017). The Isolation and Identification of Virulent Listeria monocytogenes Consumption of Local Cheeses in Şanlıurfa Province. Ataturk University Journal of