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Journals / Biotech Studies / 2023 / Cilt: 32 Sayı: 1

Neuroprotective role of chrysin against bupivacaine induced apoptosis and oxidative stress in SH-SY5Y cell line

İlknur ÇINAR AYAN
Ebru GÜÇLÜ
Pages
24–30
Publication Date
2023-06-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results of XTT analysis, the non-toxic concentration of chrysin was determined and the cells were treated with bupivacaine alone and together with this determined chrysin dose.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Chrysin, a natural flavonoid, has a strong neuroprotective effect in many neurodegenerative diseases.
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Key Findings & Data:Therefore, we aimed to investigate the neuroprotective effect of chrysin against bupivacaine-induced neurotoxicity in SH-SY5Y cells.
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Conclusion & Impact:According to the results of XTT analysis, the non-toxic concentration of chrysin was determined and the cells were treated with bupivacaine alone and together with this determined chrysin dose.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Antioxidant · Apoptosis · Bupivacaine

Abstract

Chrysin, a natural flavonoid, has a strong neuroprotective effect in many neurodegenerative diseases. Therefore, we aimed to investigate the neuroprotective effect of chrysin against bupivacaine-induced neurotoxicity in SH-SY5Y cells. According to the results of XTT analysis, the non-toxic concentration of chrysin was determined and the cells were treated with bupivacaine alone and together with this determined chrysin dose. According to the results of RT-qPCR analysis, the level of caspases increased in the group treated with only bupivacaine compared to the control group, while the expression of antioxidant enzymes decreased. When compared with the group treated with bupivacaine alone, it was determined that while the expression of caspases decreased in the group in which bupivacaine and chrysin were treated together, the expression of antioxidant enzymes increased. According to the ELISA results, SOD and CAT activities were decreased in the group treated with bupivacaine alone compared to the control group. SOD and CAT activities increased in the presence of chrysin treated with bupivacaine compared to the group treated with bupivacaine alone. The obtained data showed that chrysin may play a neuroprotective role by inducing the expression of antioxidant enzymes while inhibiting apoptosis against bupivacaine-induced neurotoxicity in SH-SY5Y cells.

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Cite This Article

AYAN, İ. Ç., GÜÇLÜ, E. (2023). Neuroprotective role of chrysin against bupivacaine induced apoptosis and oxidative stress in SH-SY5Y cell line. Biotech Studies, Cilt: 32 Sayı: 1, 24–30. https://ajindex.com/articles/948726

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References (8)

  1. Ahad, A., Ganai, A. A., Mujeeb, M., & Siddiqui, W. A. (2014). Chrysin, an antiinflammatory molecule, abrogates renal dysfunction in type 2 diabetic rats. Toxicol. Appl. Pharmacol. 279, 1e7. https://doi.org/10.1016/j.taap.2014.05.007.
  2. Ardon, A. E., Prasad, A., McClain, R. L., Melton, M. S., Nielsen, K. C., & Greengrass, R. (2019). Regional Anesthesia for Ambulatory Anesthesiologists. Anesthesiology Clinics, 37(2), 265–287. https://doi.org/10.1016/j.anclin.2019.01.005.
  3. Belli, S., Rossi, M., Molasky, N., Middleton, L., Caldwell, C., Bartow-McKenney, C., Duong, M., Chiu, J., Gibbs, E., Caldwell, A., Gahn, C., & Caruso, F. (2019). Effective and Novel Application of Hydrodynamic Voltammetry to the Study of Superoxide Radical Scavenging by Natural Phenolic Antioxidants. Antioxidants (Basel, Switzerland), 8(1), 14. https://doi.org/10.3390/antiox8010014.
  4. Bouderba, S., Sanz, M. N., Sánchez-Martín, C., El-Mir, M. Y., Villanueva, G. R., Detaille, D., & Koceïr, E. A. (2012). Hepatic mitochondrial alterations and increased oxidative stress in nutritional diabetes-prone Psammomys obesus model. Experimental Diabetes Research, 2012, 430176. https://doi.org/10.1155/2012/430176.
  5. Çınar Ayan, İ., Güçlü, E., Vural, H., & Dursun, H. G. (2022). Piceatannol induces apoptotic cell death through activation of caspase-dependent pathway and upregulation of ROS-mediated mitochondrial dysfunction in pancreatic cancer cells. Molecular Biology Reports, 49(12), 11947–11957. https://doi.org/10.1007/s11033-022-08006-8.
  6. Dhanalakshmi, C., Manivasagam, T., Nataraj, J., Justin Thenmozhi, A., & Essa, M. M. (2015). Neurosupportive Role of Vanillin, a Natural Phenolic Compound, on Rotenone Induced Neurotoxicity in SH-SY5Y Neuroblastoma Cells. Evidence-Based Complementary and Alternative Medicine: eCAM, 2015, 626028. https://doi.org/10.1155/2015/626028.
  7. El-Sisi, A. E., El-Sayad, M. E., & Abdelsalam, N. M. (2017). Protective effects of mirtazapine and chrysin on experimentally induced testicular damage in rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 95, 1059–1066. https://doi.org/10.1016/j.biopha.2017.09.022.
  8. Filho, C. B., Jesse, C. R., Donato, F., Giacomeli, R., Del Fabbro, L., da Silva Antunes, M., de Gomes, M. G., Goes, A. T., Boeira, S. P., Prigol, M., & Souza, L. C. (2015). Chronic unpredictable mild stress decreases BDNF and NGF levels and Na (+), K (+)-ATPase activity in the hippocampus and prefrontal cortex of mice: antidepressant effect of chrysin. Neuroscience, 289,