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Journals / Tıp Fakültesi Klinikleri Dergisi / 2019 / Cilt: 2 - Sayı: 3

The Effects of Thyroid Hormones on Glucokinase Enzyme Activity in diabetic Rat Liver Tissues

Duygu ŞAHİN
Nilgün ALTAN
Aylin SEPİCİ-DİNÇEL
Atilla ENGİN
Pages
105–112
Publication Date
2019-08-09
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Glucose concentration, HbA1c and thyroid hormone levels were measured in blood samples and GK enzyme activities were determined in liver tissue samples.Results: Our results showed that the glucokinase enzyme activities were significantly decreased in diabetic rat liver tissues compared to control group.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: We aimed to investigate the effcts of insulin and various doses of thyroid hormones with combine treatment on glucokinase (GK) enzyme activity, which is the one of the key enzyme in carbohydrate metabolism, diabetes and hypothyroidism.
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Key Findings & Data:Material and methods: Rats were assigned to eight groups: Group 1: control, Group 2: diabetes (DM), Group 3: DM + insulin, Group 4: thyroidectomized control, Group 5: thyroidectomized + DM, Group 6: thyroidectomized + DM + insulin, Group 7: thyroidectomized + DM + insulin + thyroid hormone (TH) (2.5μg/kg), Group 8: thyroidectomized + DM + insulin + TH (5 μg/kg).
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Conclusion & Impact:Glucose concentration, HbA1c and thyroid hormone levels were measured in blood samples and GK enzyme activities were determined in liver tissue samples.Results: Our results showed that the glucokinase enzyme activities were significantly decreased in diabetic rat liver tissues compared to control group.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Glucokinase · Thyroid hormone · Diabetes Mellitus

Özet

Objective: We aimed to investigate the effcts of insulin and various doses of thyroid hormones with combine treatment on glucokinase (GK) enzyme activity, which is the one of the key enzyme in carbohydrate metabolism, diabetes and hypothyroidism. Material and methods: Rats were assigned to eight groups: Group 1: control, Group 2: diabetes (DM), Group 3: DM + insulin, Group 4: thyroidectomized control, Group 5: thyroidectomized + DM, Group 6: thyroidectomized + DM + insulin, Group 7: thyroidectomized + DM + insulin + thyroid hormone (TH) (2.5μg/kg), Group 8: thyroidectomized + DM + insulin + TH (5 μg/kg). Glucose concentration, HbA1c and thyroid hormone levels were measured in blood samples and GK enzyme activities were determined in liver tissue samples.Results: Our results showed that the glucokinase enzyme activities were significantly decreased in diabetic rat liver tissues compared to control group. Moreover it was observed that the enzyme activities were slightly regulated by insulin. It is demonstrated that decreased GK enzyme activity in thyroidectomized diabetic rat liver was regulated by insulin and various doses of thyroid hormones.Conclusion: As a result the possible contribution of thyroid hormones to insulin effct to normalize diabetic induced changes in liver tissue has been shown.

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Cite This Article

ŞAHİN, D., ALTAN, N., SEPİCİ-DİNÇEL, A., ENGİN, A. (2019). The Effects of Thyroid Hormones on Glucokinase Enzyme Activity in diabetic Rat Liver Tissues. Tıp Fakültesi Klinikleri Dergisi, Cilt: 2 - Sayı: 3, 105–112. https://ajindex.com/articles/844620

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