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Journals / EMU Journal of Pharmaceutical Sciences / 2020 / Cilt: 3 - Sayı: 3

The effects of some organic solvents on the modified Ellman procedure for the assay of cholinesterases

Tugba ERCETİN
Açelya MAVİDENİZ
Karar SHUKUR
Ali KHOSRAVİ
Bahamehr RİAZİ
Fatameh SALAMATİ
Houra SHAHİNFAR
Mohammed NAJJARAN
Muzahem AHMED
Nasim MAHER
Negar KHAJEH
Nezhla DAVDAR
Salva SHAHİNFAR
Hayrettin Ozan GÜLCAN
Pages
153–158
Publication Date
2020-12-31
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

On the other hand, alcohols also pointed out varying degrees of deactivation of the catalytic function of cholinesterases.

📌 3 Key Takeaways

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Objective & Hypothesis:Ellman’s method is the most acknowledged procedure in order to determine the cholinesterase inhibitory potential of substances.
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Key Findings & Data:It is a quick and high-throughput screening method.
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Conclusion & Impact:On the other hand, alcohols also pointed out varying degrees of deactivation of the catalytic function of cholinesterases.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Organic solvents · acetylcholinesterase · Butyrylcholinesterase

Abstract

Ellman’s method is the most acknowledged procedure in order to determine the cholinesterase inhibitory potential of substances. It is a quick and high-throughput screening method. Therefore, it is widely used to measure the activities of original molecules and mixtures in aqueous solvents through a UV method. Regarding that many original or known substances as well as mixtures require some organic solvent to guarantee dissolution within this methodology, it becomes critical to be aware of the inhibitory potential of organic solvents. From this perspective, within the present study, it was aimed to screen the inhibitory potential of some organic solvents on human acetylcholinesterase and human butyrylcholinesterase enzymes. The results displayed the potent inhibitory function of DMSO. On the other hand, alcohols also pointed out varying degrees of deactivation of the catalytic function of cholinesterases.

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Cite This Article

ERCETİN, T., MAVİDENİZ, A., SHUKUR, K., KHOSRAVİ, A., RİAZİ, B., SALAMATİ, F., SHAHİNFAR, H., NAJJARAN, M., AHMED, M., MAHER, N., KHAJEH, N., DAVDAR, N., SHAHİNFAR, S., GÜLCAN, H. O. (2020). The effects of some organic solvents on the modified Ellman procedure for the assay of cholinesterases. EMU Journal of Pharmaceutical Sciences, Cilt: 3 - Sayı: 3, 153–158. https://ajindex.com/articles/829608

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