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Journals / Van Veterinary Journal / 2020 / Cilt: 31 - Sayı: 1

The Determination of Listeria monocytogenes in Foods with Optical Biosensors

Gıdalardan Listeria monocytogenes’in Optik Biyosensörlerle Belirlenmesi

Özmen BİBEROĞLU
Pages
50–55
Publication Date
2020-03-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

SPR and FOBs can be detected directly in the range of 102-106 cfu/ml and ≤101-103 cfu/ml, respectively.

📌 3 Key Takeaways

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Objective & Hypothesis:Listeria monocytogenes is a food-borne intracellular pathogen that is resistant to adverse conditions of food processing and is a causative agent of high mortality listeriosis disease.
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Key Findings & Data:Analysis of large food lots is limited by culture methods.
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Conclusion & Impact:SPR and FOBs can be detected directly in the range of 102-106 cfu/ml and ≤101-103 cfu/ml, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Listeria monocytogenes · Biyosensör · Yüzey plazmon rezonans

Abstract

Listeria monocytogenes is a food-borne intracellular pathogen that is resistant to adverse conditions of food processing and is a causative agent of high mortality listeriosis disease. Analysis of large food lots is limited by culture methods. Nowadays, although the conventional culture methods have been adopted as the gold standard method, they can be insufficient to control food parties that reach very large quantities. Biosensors with on-site detection potential and their characterizing with high accuracy and precision offers many opportunities for real-time estimation of industrial contamination. As the most promising technique for rapid detection of L.monocytogenes, ones based on surface plasmon resonance (SPR) and fiber optic biosensors (FOBs) where direct or indirect detections can be made by detecting the interaction of the biomarker element with the optical field without marking or labeling, have come to the fore. SPR and FOBs can be detected directly in the range of 102-106 cfu/ml and ≤101-103 cfu/ml, respectively.

Özet

Listeria monocytogenes gıda kaynaklı intrasellüler patojen olarak gıda işlemenin olumsuz koşullarına dayanıklı ve yüksek mortaliteli listerioz hastalığının etkenidir. Büyük gıda partilerinin kültür yöntemleri ile analizi sınırlıdır. Günümüzde, geleneksel kültür yöntemleri altın standart yöntem olarak benimsenmesine karşın, çok büyük miktarlara ulaşan gıda partilerini kontrol etmede yetersiz olabilirler. Biyosensörlerin yerinde tespit potansiyeli ile yüksek doğruluk ve hassasiyetle karakterize edilmeleri, endüstriyel kontaminasyonları gerçek zamanlı tahminde birçok fırsat sunmaktadır. Listeria monocytogenes, hızlı tespitte en umut verici teknik olarak işaretleme veya etiketleme yapılmadan optiksel alanla biyotanıyıcı eleman etkileşiminin optik sinyallerle belirlenerek doğrudan veya endirekt tespitlerin yapılabildiği yüzey plazmon rezonans (SPR) ve fiber optik biyosensör (FOB) esaslı olanları ön plana çıkmıştır. SPR ve FO biyosensörleri ile sırasıyla 102-106 kob/ml ve ≤101-103 kob/ml aralıklarında doğrudan tespit yapılabilmektedir.

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Cite This Article

BİBEROĞLU, Ö. (2020). The Determination of Listeria monocytogenes in Foods with Optical Biosensors. Van Veterinary Journal, Cilt: 31 - Sayı: 1, 50–55. https://ajindex.com/articles/82723

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