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Journals / Recep Tayyip Erdoğan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi / 2021 / Cilt: 2 - Sayı: 2

Bioinformatic Analysis and Expression Profile of MDR Subfamily of ABC Proteins from Oyster Mushroom (Pleurotus ostreatus) Genome

Kayın Mantarı (Pleurotus ostreatus) Genomunda ABC Proteinlerinden MDR Alt Ailesinin Biyoinformatik Analizi ve İfade Profili

Hatice Kübra DÜZEL
Birsen ÇAKIR AYDEMİR
Pages
72–82
Publication Date
2021-12-31
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In addition, within the scope of this study, the expression levels of PoMDR genes in the stem and cap were examined for the first time and it was determined that the expression levels of these genes had similar profiles, but the expression levels of these genes were higher in the cap.

📌 3 Key Takeaways

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Objective & Hypothesis:ATP-binding cassette proteins (ABC), one of the largest protein families involved in the transport of substances by consuming ATP in many different metabolic reactions in the cell membrane.
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Key Findings & Data:In this study, 8 MDR protein-coding genes (PoMDRs) belonging to the ABCB gene family, which is a sub-class of ABC proteins, were determined by bioinformatic analysis of the beech mushroom (Pleurotus osteradus) genome.
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Conclusion & Impact:In addition, within the scope of this study, the expression levels of PoMDR genes in the stem and cap were examined for the first time and it was determined that the expression levels of these genes had similar profiles, but the expression levels of these genes were higher in the cap.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:ABC Kaset Taşıyıcıları · MDR Alt Ailesi · mRNA İfadesi

Abstract

ATP-binding cassette proteins (ABC), one of the largest protein families involved in the transport of substances by consuming ATP in many different metabolic reactions in the cell membrane. Soluble ABC proteins, on the other hand, are not involved in transmembrane transport, but play an important role in cellular processes, such as ribosome biogenesis and mRNA translation. Multidrug resistance (MDR) subfamily, which is a subfamily of ABC proteins, plays a role in the transport of antimicrobial peptides, lipid transport, pheromone transport, mitochondrial porphyrin uptake, multidrug resistance, eukaryotic peptide transport, antigen processing, protection from oxidative stress, heavy metal resistance, etc. In this study, 8 MDR protein-coding genes (PoMDRs) belonging to the ABCB gene family, which is a sub-class of ABC proteins, were determined by bioinformatic analysis of the beech mushroom (Pleurotus osteradus) genome. And a phylogenetic tree was formed with the proteins encoded by these genes. In addition, within the scope of this study, the expression levels of PoMDR genes in the stem and cap were examined for the first time and it was determined that the expression levels of these genes had similar profiles, but the expression levels of these genes were higher in the cap.

Özet

Hücre zarında birçok farklı metabolik yolu kontrol eden yapılardan biri olan ve en büyük protein ailelerinden birini oluşturan ATP bağlayan kaset taşıyıcı proteinleri (ABC) ATP enerjisini kullanarak madde taşınımında görev alırlar. Çözünür ABC proteinleri transmembran taşınmasına dahil değildir, ancak hücresel süreçlerde, örneğin ribozom biyojenezi ve mRNA translasyonunda önemli bir rol oynamaktadır. ABC proteinlerinin bir alt ailesi olan çoklu ilaç direnci (MDR) alt ailesi, antimikrobiyal peptitler, lipit taşınması, feromon taşınması, mitokondriyal porfirin alımı, ökaryotik peptit taşınması, antijenlerin işlenmesi, oksidatif stresden korunma, ağır metal dayanıklılığı, v.b. olaylarda rol oynamaktadır. Bu çalışmada kayın mantarı (Pleurotus osteradus) genomunun biyoinformatik analizi ile ABC proteinlerinin bir alt üyesi olan ABCB gen ailesine ait 8 tane MDR protein kodlayan gen (PoMDRs) belirlenmiştir. PoMDRs genlerinin kodladığı proteinler ile filogenetik ağaç oluşturulmuştur. Ayrıca bu çalışma kapsamında ilk defa sap ve şapkada PoMDR genlerinin ifade seviyeleri incelenmiş ve bu genlerin ifade düzeylerinin benzer profillere sahip oldukları ancak bu genlerin ifade seviyelerinin şapkada daha fazla olduğu tespit edilmiştir.

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Cite This Article

DÜZEL, H. K., AYDEMİR, B. Ç. (2021). Bioinformatic Analysis and Expression Profile of MDR Subfamily of ABC Proteins from Oyster Mushroom (Pleurotus ostreatus) Genome. Recep Tayyip Erdoğan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 2 - Sayı: 2, 72–82. https://ajindex.com/articles/797080

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