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Journals / New Trends in Medicine Sciences / 2020 / Cilt: 1 - Sayı: 2

The Effect of Apilarnil on The Autophagia Against Lipolysaccarite-Based Sepsıs in Liver

Züleyha DOĞANYİĞİT
Betül KÖKLÜ
Arda UNER
Aslı OKAN
Emin KAYMAK
Sibel SİLİCİ
Pages
84–89
Publication Date
2020-09-30
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

When apilarnil was applied with LPS, it was determined that there was an increase in Beclin-1 level (p> 0.05) and a decrease in P62 levels (p

📌 3 Key Takeaways

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Objective & Hypothesis:Sepsis, triggered by highly bacterial lipopolysaccharide (LPS) endotoxins, exhibits high morbidity and mortality despite medical advances.
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Key Findings & Data:In this study, it is aimed to reveal the potential protective effect of apilarnil on the autophagy mechanism in the endotoxic liver model LPS-induced. 64 male Sprague dawley rats weighing 200-250; control, apilarnil treated groups (0.2, 0.4 and 0.8 g / kg), LPS (30 mg / kg) group and LPS + apilarnil treated groups (LPS + 0.2 g / kg, LPS + 0.4 g / kg and LPS + 0.8 g / kg) are randomly divided into eight groups.
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Conclusion & Impact:When apilarnil was applied with LPS, it was determined that there was an increase in Beclin-1 level (p> 0.05) and a decrease in P62 levels (p
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Beclin-1 · LC3 · P62

Abstract

Sepsis, triggered by highly bacterial lipopolysaccharide (LPS) endotoxins, exhibits high morbidity and mortality despite medical advances. Damage to the liver occurs due to the production of highly reactive oxygen compounds (ROS) and the release of various proinflammatory cytokines. It is suggested that autophagy, which regulates inflammation and selectively destroys damaged mitochondria, suppresses apoptosis and provides a possible protective mechanism in the endotoxic liver. However, apilarnil, a bee product, is known to have high antioxidant activity and positive effects against various diseases thanks to its polyphenols. In this study, it is aimed to reveal the potential protective effect of apilarnil on the autophagy mechanism in the endotoxic liver model LPS-induced. 64 male Sprague dawley rats weighing 200-250; control, apilarnil treated groups (0.2, 0.4 and 0.8 g / kg), LPS (30 mg / kg) group and LPS + apilarnil treated groups (LPS + 0.2 g / kg, LPS + 0.4 g / kg and LPS + 0.8 g / kg) are randomly divided into eight groups. Beclin-1, LC3 and P62 proteins were analyzed immunohistochemically in order to determine the activity level of autophagy pathway in the liver tissues taken after the completion of the experiment protocol. The data obtained showed that Beclin-1 immunoreactivity decreased while LC3 and P62 expression increased in the tissues of the LPS group compared to the control group. When apilarnil was applied with LPS, it was determined that there was an increase in Beclin-1 level (p> 0.05) and a decrease in P62 levels (p

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Cite This Article

DOĞANYİĞİT, Z., KÖKLÜ, B., UNER, A., OKAN, A., KAYMAK, E., SİLİCİ, S. (2020). The Effect of Apilarnil on The Autophagia Against Lipolysaccarite-Based Sepsıs in Liver. New Trends in Medicine Sciences, Cilt: 1 - Sayı: 2, 84–89. https://ajindex.com/articles/793964

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References (1)

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