Phylogenetic Relationships of Turbot Species (Scophthalmidae) Inferred from the Mitochondrial COIII Gene and Morphological Characters

Objective & Hypothesis:In this study, the validity, distribution and structure of three turbot species, Scophthalmus maeoticus, S. maximus, S. rhombus, belong to Scophthalmidae family in Turkish, Bulgarian and Russian coastal waters were determined with mtDNA sequencing of Cytochrome c oxidase subunit III (COIII).

📊

Key Findings & Data:The sequencing of the COIII region revealed 8 bp variable and 6 bp parsimony informative sites between all turbot species.

💡

Conclusion & Impact:The overall genetic and haplotype diversities among all turbot species were found to be 0.004109 and 0.7655, respectively.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Turbot · phylogeny · mtDNA sequencing

Abstract

In this study, the validity, distribution and structure of three turbot species, Scophthalmus maeoticus, S. maximus, S. rhombus, belong to Scophthalmidae family in Turkish, Bulgarian and Russian coastal waters were determined with mtDNA sequencing of Cytochrome c oxidase subunit III (COIII). The sequencing of the COIII region revealed 8 bp variable and 6 bp parsimony informative sites between all turbot species. The overall genetic and haplotype diversities among all turbot species were found to be 0.004109 and 0.7655, respectively. Genetic distance analysis showed that the highest nucleotide differences was observed between S. maximus and S. rhombus species with a value of 0.09620 and, the lowest value (0.02482) was observed between S. maximus and S. maeoticus species. Neighbor Joining and Maximum Parsimony phylogenetic approaches resulted in the similar tree topologies that S. maximus and S. maeoticus were found as sister group, whereas S. rhombus was more divergent from this group. The mtDNA COIII gene is a useful genetic marker for species specific identification of the genus Scophthalmus due to its inter-specific heterogeneity producing a species-specific pattern. In morphological analyses, S. rhombus was most differentiated from S. maximus and S. maeoticus. The genetic data was supported by the detected morphometric variations among the turbot species.

Keywords

Turbot phylogeny mtDNA sequencing morphology the Black Sea the Marmara Sea.

🔖

Cite This Article

TURAN, C., IVANOVA, P., GÜRLEK, M., YAĞLİOĞLU, D., ERGÜDEN, D., KARAN, S., DOĞDU, S. A., UYAN, A., ÖZTÜRK, B., NİKOLOV, V., RAYKOV, V., DOBROVOLOV, İ., KHANAYCHENKO, A. (2019). Phylogenetic Relationships of Turbot Species (Scophthalmidae) Inferred from the Mitochondrial COIII Gene and Morphological Characters. Natural and Engineering Sciences, Cilt: 4 - Sayı: 1, 28–41. https://ajindex.com/articles/793205

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (12)

Avise, J. C. (Ed.) (1994).
Molecular Markers, Natural History and Evolution. Chapman and Hall, New York, NY. pp. 511. Azevedo, M. F. C., Oliveira, C., Pardo, B. G., Martinez, P., & Foresti, F. (2008). Phylogenetic analysis of the order Pleuronectiformes (Teleostei) based on sequences of 12S and 16S mitochondrial genes. Genetics and Molecular Biology, 31, 284-292.
Bailly, N. & Chanet, B. (2010). Scophthalmus Rafinesque, 1810: the valid generic name for the turbot, S. maximus (Linnaeus, 1758) [Pleuronectiformes: Scophthalmidae]. Cybium, 34(3), 257-261.
Blanquer, A., Alayse, J. P., Berrada‐Rkhami, O., & Berrebi, P. (1992). Allozyme variation in turbot (Psetta maxima) and brill (Scophthalmus rhombus) (Osteichthyes, Pleuronectoformes, Scophthalmidae) throughout their range in Europe. Journal of Fish Biology, 41(5), 725-736.
Brooks, D. R., & McLennan, D. A. (2012). The Nature of Diversity: An Evolutionary Voyage of Discovery. University of Chicago Press.
Brown, J. M., Pellmyr, O., Thompson, J. N., & Harrison, R. G. (1994). Phylogeny of Greya (Lepidoptera: Prodoxidae), based on nucleotide sequence variation in mitochondrial cytochrome oxidase I and II: congruence with morphological data. Molecular Biology and Evolution, 11(1), 128-141.
Chanet, B. (2003). Interrelationships of scophthalmid fishes (Pleuronectiformes: Scophthalmidae). Cybium, 27(4), 275-286.
Durand, P., Pointier, J. P., Escoubeyrou, K., Arenas, J., Yong, M., Amarista, M., Bargues, M. D., Mas-Coma, S., & Renaud, F. (2002). Occurrence of a sibling species complex within neotropical lymnaeids, snail intermediate hosts of fascioliasis. Acta Tropica, 83, 233-240.
Elliott, N. C., Farrell, J. A., Gutierrez, A. P., van Lenteren, J. C., Walton, M. P., & Wratten, S. (1995). Integrated pest management. Springer Science & Business Media. Eschmeyer, W. N. (2011). Catalog of Fishes: Genera, Species, References. (http://research.calacademy.org/research/ichthyology/catalog/fishcatmain.asp). Accessed on 07 March 2016.
Evseenko, S. A. (2003). An annotated catalogue of pleuronectiform fishes (order Pleuronectiformes) of the seas of Russia and adjacent countries. Journal of Ichthyology, 43(1), 57-74.
Felsenstein, J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39(4), 783-791.
Feng, Y., Jing, L., Peijun, Z., & Jianhai, X. (2005). Preliminary study on mitochondrial 16S rRNA gene sequences and phylogeny of flatfishes (Pleuronectiformes). Chinese Journal of Oceanology and Limnology,