SavedAdd your journal

Journals / Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2017 / Cilt: 8 - Sayı: Ek (Suppl.) 1

Analysis of Time-Dependent Alteration of HIF-2α Expression in Breast Cancer Cells

Meme Kanseri Hücrelerinde Zamana Bağlı HIF-2α İfadesi Değişiminin Analizi

Muzaffer DÜKEL
Kevin D. BROWN
Pages
245–251
Publication Date
2017-11-21
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The aim of this study is that to investigate the effect of time dependent hypoxia exposure on HIF-2α expression in different breast cancer cells and normal breast epithelial cells.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Breast cancer is the most commonly diagnoised cancer type in women and the number of cases observed in Turkey has increased day by day.
📊
Key Findings & Data:By the effect of HIF transcription factors, cancer cells migrate to different tissues and organs by stimulating vascular development.
💡
Conclusion & Impact:The aim of this study is that to investigate the effect of time dependent hypoxia exposure on HIF-2α expression in different breast cancer cells and normal breast epithelial cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Meme kanseri · hipoksiya · HIF-2a

Abstract

Breast cancer is the most commonly diagnoised cancer type in women and the number of cases observed in Turkey has increased day by day. In many types of cancer including breast cancer, tumor tissues are exposed to oxygen deficiency while growing in areas distant from blood vessels. Against this condition, knew as hypoxia, tissues respond via increasing the expression of transcription factors called Hypoxia inducible factor (HIF). By the effect of HIF transcription factors, cancer cells migrate to different tissues and organs by stimulating vascular development. Therefore, it is important to know the change of HIF transcription factors in hypoxia process. HIF-2α is a transcription factor, which is over expressed during hypoxic conditions and have varies the level of expression in different cancer types. The aim of this study is that to investigate the effect of time dependent hypoxia exposure on HIF-2α expression in different breast cancer cells and normal breast epithelial cells.

Özet

Meme kanseri kadınlarda en sık rastlanan kanser türüdür veTürkiye’de gözlemlenen vaka sayısı her geçen gün artmaktadır. Meme kanserinin içinde yer aldığı birçok kanser türünde, kanserli doku kan damarlarından uzak bölgelerde büyürken oksijen eksikliğine maruz kalır. Hipoksiya olarak adlandırılan bu duruma karşı dokular Hipoksi ile indüklenebilir faktör (HIF) transkripsiyon faktörlerinin ifade artışı ile cevap verirler. HIF transkripsiyon faktörlerinin etkisiyle kanserli hücreler, damar gelişimini uyararak farklı doku ve organlara göç ederler. Bu nedenle HIF transkripsiyon faktörlerinin hipoksiya sürecindeki değişiminin bilinmesi önemlidir. HIF-2α hipoksik koşullar altında ifadesi artan bir transkripsiyon faktörüdür ve farklı kanser türlerinde ifade düzeyi değişiklik göstermektedir. Bu çalışmanın amacı farklı meme kanseri ve normal meme epiteli hücrelerinde farklı sürelerde hipoksiya uygulamasının HIF-2α ifadesinin değişimi üzerinde etkisinin araştırılmasıdır.

🔖

Cite This Article

DÜKEL, M., BROWN, K. D. (2017). Analysis of Time-Dependent Alteration of HIF-2α Expression in Breast Cancer Cells. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 8 - Sayı: Ek (Suppl.) 1, 245–251. https://ajindex.com/articles/790244

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Bardos, J. I., Ashcroft, M. (2005). Negative and positive regulation of HIF-1: a complex network. Biochimica et Biophysica Acta 1755(2): 107–120. http://doi.org/10.1016/j.bbcan.2005.05.001
  2. Beckmann, M. W., Niederacher, D., Schnurch, H. G., Gusterson, B. A., Bender, H. G. (1997). Multistep carcinogenesis of breast cancer and tumour heterogeneity. Journal of Molecular Medicine 75(6): 429–439.
  3. Bobarykina, A. I., Minchenko, D. O., Opentanova, I. L., Kovtun, O. O., Komisarenko, S. V, Esumi, H., Minchenko, O. H. (1999). [HIF-1alpha, HIF-2alpha and VHL mRNA expression in different cell lines during hypoxia]. Ukrains’kyi biokhimichnyi zhurnal 78(2): 62–72.
  4. Brown, K. D., Lataxes, T. A., Shangary, S., Mannino, J. L., Giardina, J. F., Chen, J., Baskaran, R. (2000). Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism. The Journal of Biological Chemistry 275(9): 6651–6656.
  5. Burroughs, S. K., Kaluz, S., Wang, D., Wang, K., Van Meir, E. G., Wang, B. (2013). Hypoxia inducible factor pathway inhibitors as anticancer therapeutics. Future Medicinal Chemistry 5(5): 553–572. http://doi.org/10.4155/fmc.13.17
  6. Calzada, M. J., del Peso, L. (2007). Hypoxia-inducible factors and cancer. Clinical & Translational Oncology: Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 9(5): 278–289.
  7. Cavadas, M. A. S., Mesnieres, M., Crifo, B., Manresa, M. C., Selfridge, A. C., Scholz, C. C., Taylor, C. T. (2015). REST mediates resolution of HIF-dependent gene expression in prolonged hypoxia, Scientific Reports 5: 17851. http://dx.doi.org/10.1038/srep17851
  8. Dukel, M., Streitfeld, W. S., Tang, T. C. C., Backman, L. R. F., Ai, L., May, W. S., Brown, K. D. (2016). The Breast Cancer Tumor Suppressor TRIM29 Is Expressed via ATM-dependent Signaling in Response to Hypoxia. The Journal of Biological Chemistry 291(41): 21541–21552. http://doi.org/10.1074/jbc.M116.730960
  9. Hamidian, A., von Stedingk, K., Munksgaard Thoren, M., Mohlin, S., Pahlman, S. (2015). Differential regulation of HIF-1alpha and HIF-2alpha in neuroblastoma: Estrogen-related receptor alpha (ERRalpha) regulates HIF2A transcription and correlates to poor outcome. Biochemical and Biophysical Research Communications 461(3): 560–567.
  10. Hu, C.-J., Wang, L.-Y., Chodosh, L. A., Keith, B., Simon, M. C. (2003). Differential Roles of Hypoxia-Inducible Factor 1α (HIF-1α) and HIF-2α in Hypoxic Gene Regulation. Molecu