Phytochemical Profiling and Evaluation of Marrubium sivasense Aytaç, Akgül & Ekici for Antioxidant Activity and Inhibition Effects on α-Amylase, Lipoxygenase, Xanthine Oxidase and Tyrosinase Enzymes
The extract demonstrated hypoglycemic activity (Inh. 49.67±1.09 %.) via inhibition of porcine pancreatic α-amylase, the antiinflammatory effect of the extract tested via inhibition of 5-LOX was found as Inh 18.71±0.74 %, however no antityrosinase activity was found.
📌 3 Key Takeaways
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Objective & Hypothesis:Methanol extract of Marrubium. sivasense Aytaç, Akgül & Ekici was analyzed with LC-MS/MS technique.
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Key Findings & Data:Antioxidant properties of the extract was determined by DPPH free radical scavenging activity (IC50 0.34 mg/mL), trolox equivalent antioxidant capacity (TEAC) (2.3 mM±0.04), xanthine-xanthine oxidase(XO) inhibitory effect (16.54 %). and.
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Conclusion & Impact:The extract demonstrated hypoglycemic activity (Inh. 49.67±1.09 %.) via inhibition of porcine pancreatic α-amylase, the antiinflammatory effect of the extract tested via inhibition of 5-LOX was found as Inh 18.71±0.74 %, however no antityrosinase activity was found.
Methanol extract of Marrubium. sivasense Aytaç, Akgül & Ekici was analyzed with LC-MS/MS technique. Forsythoside B, verbascoside and leucoseptoside were identified as main compounds. Antioxidant properties of the extract was determined by DPPH free radical scavenging activity (IC50 0.34 mg/mL), trolox equivalent antioxidant capacity (TEAC) (2.3 mM±0.04), xanthine-xanthine oxidase(XO) inhibitory effect (16.54 %). and. B-carotene bleaching (Inh. 43±1.26 %) assays. The extract demonstrated hypoglycemic activity (Inh. 49.67±1.09 %.) via inhibition of porcine pancreatic α-amylase, the antiinflammatory effect of the extract tested via inhibition of 5-LOX was found as Inh 18.71±0.74 %, however no antityrosinase activity was found.
GÖĞER, F., ÖZEK, G., TEKİN, M., YUR, S., ÖZEK, T. (2019). Phytochemical Profiling and Evaluation of Marrubium sivasense Aytaç, Akgül & Ekici for Antioxidant Activity and Inhibition Effects on α-Amylase, Lipoxygenase, Xanthine Oxidase and Tyrosinase Enzymes. Journal of the Turkish Chemical Society Section A: Chemistry, Cilt: 6 - Sayı: 3, 281–292. https://ajindex.com/articles/781264
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