SavedAdd your journal

Journals / Journal of Research in Veterinary Medicine / 2019 / Cilt: 38 - Sayı: 2

Detection of Shiga-Toxin Producing E.coli (STEC), Enteropathogenic E.coli (EPEC) And Enterotoxigenic E.coli (ETEC) From Animals By M-PCR

Esra BÜYÜKCANGAZ
Mohammed KHİDER
Ayşin ŞEN
Murat CENGİZ
Songül SONAL
Pages
88–94
Publication Date
2019-12-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This research investigated the presence of virulence genes encoding F41, K99, eae, Stx1, Stx2 and STa and the antimicrobial resistance of animal Escherichia coli (E. coli) isolates.
📊
Key Findings & Data:Clinical isolates (n:233) were evaluated from faecal samples of cattle, sheep, goats, horses, cats and dogs collected between the years of 2010 to 2015 from Turkey.
💡
Conclusion & Impact:The results showed that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC).
🔬Design:Research Article
📍Scope:2010 Verileri
⚙️Variables:Antimicrobial susceptibility · E. coli · Pcr

Abstract

This research investigated the presence of virulence genes encoding F41, K99, eae, Stx1, Stx2 and STa and the antimicrobial resistance of animal Escherichia coli (E. coli) isolates. Clinical isolates (n:233) were evaluated from faecal samples of cattle, sheep, goats, horses, cats and dogs collected between the years of 2010 to 2015 from Turkey. Enterohaemorrahic E.coli (EHEC) O157:H7 was detected by using cefixime tellurite sorbitol MacConkey agar (CT-SMAC) and Wellcollex E. coli (Remel®). The Kirby-Bauer disc diffusion test was performed to detect the resistance pattern of the isolates to ampicillin, Amoxycillin/clavulanic acid, enrofloxacin, ceftiofur, trimethoprim/sulfamethoxazole and tetracycline. The results showed that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC). Enterotoxigenic E. coli (ETEC) was detected in samples from cattle (0.9%) and sheep (12%). Enteropathogenic E.coli (EPEC) was detected in samples from cattle (0.9%) and dogs (11.4%). EHEC O157:H7 was not detected any of the isolates. Among all E. coli isolates that carried at least one virulence gene, 8 (19%) were resistant to more than three antimicrobials, 7 (16.7%) were resistant to at least one antimicrobial and 27 (64.3%) were susceptible to all antimicrobials.

🔖

Cite This Article

BÜYÜKCANGAZ, E., KHİDER, M., ŞEN, A., CENGİZ, M., SONAL, S. (2019). Detection of Shiga-Toxin Producing E.coli (STEC), Enteropathogenic E.coli (EPEC) And Enterotoxigenic E.coli (ETEC) From Animals By M-PCR. Journal of Research in Veterinary Medicine, Cilt: 38 - Sayı: 2, 88–94. https://ajindex.com/articles/778583

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Jorgensen JH, Pfaller MA, Carroll K C, Funke G, Landry ML, Richter SS, Warnock DW (2015):Manual of Clinical Microbiology. Volume 1.11th edition, ASM Press, Washington, DC, pp: 685-713.Chapter 37. Esherichia, Shigella and Salmonella. Strockbine N.A., Bopp C.A., Fields P.I., Kaper J.B., Nataro J.P
  2. Gyles CL (2007): Shiga toxin-producing Escherichia coli: an overview. Journal of Animal Science 85, 45-62
  3. Nataro JP, Kaper JB (1998): Diarrheagenic Escherichia coli. Clinical Microbiology Reviews 11,142-201
  4. Gyles CL, Fairbrother JM (2010): Escherichia coli, in: Gyles, C. L., Prescott, J. F., Songer, J. G. and Thoen, C. O. (eds), Pathogenesis of Bacterial Infections in Animals.4th ed.Wiley-Blackwell, Ames, IA, U.S.A, 267-308
  5. Kaper JB, Nataro JP, Mobley HL (2004): Pathogenic Escherichia coli. Nature Reviews Microbiology 2, 123-140
  6. Law D (2000): Virulence factors of Escherichia coli O157 and other shiga toxin-producing E.coli. Journal of Applied Microbiology 88, 729-745
  7. Blanco M, Blanco J, Blanco JE, Ramos J (1993): Enterotoxigenic, verotoxigenic, and necrotoxigenic Escherichia coli isolated from cattle in Spain. Am. J. Vet. Res. 54, 1446-1451
  8. Pitout, JD, Laupland KB (2008): Extended-spectrum beta-lactamase-producing Enterobacteriaceae: an emerging public health concern. Lancet Infectious Disease 8, 159-166
  9. Sawant AA, Hegde NV, Straley BA, Donaldson SC, Love BC, Knabel SJ, Jayarao BM (2007): Antimicrobial-Resistant Enteric Bacteria from Dairy Cattle. Applied and Environmental Microbiology 73, 156-163
  10. Franck SM, Bosworth BT, Moon HW (1998): Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxin- producing Escherichia coli strains from calves. Journal of Clinical Microbiology 36, 1795-1797
  11. Anonymous: European Committee on Antimicrobial Susceptibility Testing Breakpoint tables for interpretation of MICs and zone diameters Version 7.1, valid from 2017-03-10
  12. Guler L, Gunduz K, Ok U (2008): Virulence Factors and Antimicrobial Susceptibility of Escherichia coli Isolated from Calves in Turkey. Zoonoses and Public Health. 55, 249-257
  13. Galli L, Miliwebsky E., Irino K, Leotta G, Rivas M (2010): Virulence profile comparison between LEE-negative Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle and humans. Veterinary Microbiology 143, 307-313
  14. Nguyen TD, Vo TT, V