Journals / Journal of Research in Veterinary Medicine / 2020 / Cilt: 39 - Sayı: 2

Easy Isolation, Propagation, Characterization and Multilineage Differentiation of Equine Amniotic Fluid Derived Stem Cells

At Amniyotik Sıvı Kaynaklı Kök Hücrelerin Kolay İzolasyonu, Çoğaltılması, Karakterizasyonu ve Çoklu Farklılaştırması

Pages
135–142
Publication Date
2020-12-30
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further extensive and comprehensive molecular studies are important to conclude the precious procedures for clinical approach of amniotic fluid derived stem cells.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Easy harvesting and handy application of stem cells therapy want convenient and alternative source.
📊
Key Findings & Data:Equine amniotic fluid derived mesenchymal stem cells make hope for scientists as of no ethical concern and of higher proliferation and differentiation potency.
💡
Conclusion & Impact:Further extensive and comprehensive molecular studies are important to conclude the precious procedures for clinical approach of amniotic fluid derived stem cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:amniyotik sıvı · çoklu farklılaştırma · kök hücre

Abstract

Easy harvesting and handy application of stem cells therapy want convenient and alternative source. Equine amniotic fluid derived mesenchymal stem cells make hope for scientists as of no ethical concern and of higher proliferation and differentiation potency. Equine AFSCs were isolated, cultured and propagated for characterization of potency of growth and multilineage differentiation study. Successful harvesting and optimum differentiation of equine AFSCs into osteogenic, adipogenic and neurospheres through this study could make a ground for their clinical application for various purposes. Further extensive and comprehensive molecular studies are important to conclude the precious procedures for clinical approach of amniotic fluid derived stem cells.

Özet

İzolasyon ve uygulama kolaylığı açısından kök hücre tedavisi alanında uygun bir alternatif hücre kaynağı gerekmektedir. At amniyotik sıvı kaynaklı kök hücreler (AASKH) proliferasyon kabiliyetleri, yüksek farklılaşma kabiliyetleri ve izolasyonunda etik sorunların olmaması bakımından bilim insanları için ön plana çıkmaktadır. Çalışmada at amniyotik sıvı kaynaklı kök hücreler izole edilmiş, kültüre edilmiş ve karakterizasyonu, çoğalma kabiliyetleri ve çoklu farklılaşma potansiyelleri incelenmiştir. AASKH’lerin başarılı izolasyonunun ardından bu hücrelerin osteojenik, adipojenik farklılaştırma ve nörosfer oluşturma bakımından optimal özellikler göstermesi bu hücre tipini birçok amaç için yapılacak klinik uygulamalarda uygun bir aday haline getirmektedir. AASKH’lerin klinik uygulamalarda kullanılabilirliğini görebilmek adına bu hücre tipi hakkında yapılacak daha detaylı moleküler çalışmalar yol gösterici olacaktır.

🔖

Cite This Article

KIBRIA, A. G., NAWAZ, S., AKKAYA, Ö. Ö., DİKMEN, T., YAĞCI, A. (2020). Easy Isolation, Propagation, Characterization and Multilineage Differentiation of Equine Amniotic Fluid Derived Stem Cells. Journal of Research in Veterinary Medicine, Cilt: 39 - Sayı: 2, 135–142. https://ajindex.com/articles/778562

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Iacono E, Brunori L, Pirrone A, et al. Isolation, characterization and differentiation of mesenchymal stem cells from amniotic fluid, umbilical cord blood and Wharton's jelly in the horse. Reproduction. April 1, 2012 2012
  2. 143(4):455-468. 2.Tsai MS, Lee JL, Chang YJ, Hwang SM. Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel two-stage culture protocol. Hum Reprod. 2004
  3. Atala A. Amniotic Fluid Derived Pluripotent Cells. In: Lanza R, Gearhart J, Hogan B, et al., eds. Essentials of Stem Cell Biology. 2nd ed. Canada: Elsevier
  4. Nelson TJ, Martinez-Fernandez A, Yamada S, Ikeda Y, Perez-Terzic C, Terzic A. Induced pluripotent stem cells: advances to applications. Stem cells and cloning: advances and applications. 2010/01// 2010
  5. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. Aug 25 2006
  6. 126(4):663-676. 6.Park IH, Zhao R, West JA, et al. Reprogramming of human somatic cells to pluripotency with defined factors. Nature. Jan 10 2008
  7. 451(7175):141-146. 7.Liao J, Cui C, Chen S, et al. Generation of induced pluripotent stem cell lines from adult rat cells. Cell Stem Cell. Jan 9 2009
  8. 4(1):11-15. 8.Liu H, Zhu F, Yong J, et al. Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts. Cell stem cell. 2008/12// 2008
  9. 3(6):587-590. 9.Nagy K, Sung HK, Zhang P, et al. Induced pluripotent stem cell lines derived from equine fibroblasts. Stem Cell Rev Rep. Sep 2011
  10. 7(3):693-702. 10.Nelson TJ, Faustino RS, Chiriac A, Crespo-Diaz R, Behfar A, Terzic A. CXCR4+/FLK-1+ Biomarkers Select a Cardiopoietic Lineage from Embryonic Stem Cells. Stem Cells. 2008
  11. 26(6):1464-1473. 11.Horwitz EM, Prockop DJ, Fitzpatrick LA, et al. Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta. Nat Med. Mar 1999
  12. 5(3):309-313. 12.Yu L. Human Amniotic Fluid-Derived and Amniotic Membrane-Derived Stem Cells. In: Zhao RC, ed. Stem Cells: Basics and Clinical Translation. Vol 1. Dordrecht: Springer
  13. Seo MJ, Suh SY, Bae YC, Jung JS. Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochem Biophys Res Commun. Mar 4 2005
  14. 328(1):258-264. 14.Romanov YA, Svintsitskaya VA, Smirnov VN. Searching for Alternative Sources of Postnatal Human Mesenchymal Stem Cells: Candidate MSC-Like Cells from Umbilical Cord. S