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Journals / Journal of Research in Veterinary Medicine / 2022 / Cilt: 41 - Sayı: 1

The Effect of L-Carnitine Added Extenders on Spermatological Parameters of Goat Sperma Post-Thawing

L-Karnitin İlave Edilmiş Sulandırıcıların Dondurma-Çözdürme Sonrası Teke Spermasının Spermatolojik Parametleri Üzerine Etkisi

Ahmet AKTAR
Selim ALÇAY
Pages
37–42
Publication Date
2022-07-26
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Motility values post thaw; LC5 and LC7,5 groups had a possitive effect on compared to the the control group (P

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, it was aimed to evaluate the effects of L-carnitine (LC) on goat spermatozoa after thawing.
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Key Findings & Data:Diluted with diluents added at different concentrations of LC (2.5mM /5 mM/ 7.5mM/10 mM) and without (control) and frozen.
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Conclusion & Impact:Motility values post thaw; LC5 and LC7,5 groups had a possitive effect on compared to the the control group (P
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Goat · semen · Cryopreservation

Abstract

In this study, it was aimed to evaluate the effects of L-carnitine (LC) on goat spermatozoa after thawing. Sperm samples from sexually mature goat were pooled and divided into five equal volumes. Diluted with diluents added at different concentrations of LC (2.5mM /5 mM/ 7.5mM/10 mM) and without (control) and frozen. Motility values post thaw; LC5 and LC7,5 groups had a possitive effect on compared to the the control group (P

Özet

Bu çalışmada, L-karnitinin (LC) eritme sonrası teke spermatozoonu üzerindeki etkilerinin değerlendirmesi amaçlandı. Cinsel olarak olgun tekelerden elde edilen sperm örnekleri pooling yapıldı ve beş eşit hacme bölündü. Farklı konsantrasyonlarda LC içeren (2.5mM /5mM/ 7.5mM/ 10 mM) ve içermeyen (kontrol) sulandırıcılar ile sulandırıldı ve donduruldu. Eritme sonrası motilite değerleri; LC5 ve LC7,5 gruplarında kontrol grubuna göre daha üstün bulundu (P

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Cite This Article

AKTAR, A., ALÇAY, S. (2022). The Effect of L-Carnitine Added Extenders on Spermatological Parameters of Goat Sperma Post-Thawing. Journal of Research in Veterinary Medicine, Cilt: 41 - Sayı: 1, 37–42. https://ajindex.com/articles/778528

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References (13)

  1. Holt WV, Penfold LM, Chenoweth P, & Lorton S. Fundamental and practical aspects of semen cryopreservation. In P. Chenoweth, & S. Lorton (Eds.), Animal andrology: Theories and applications (pp. 76–79). Wallingford, UK: CAB International. 2014
  2. Ortman K, Rodriguez-Martinez H. Membrane Damage during Dilution, Cooling and Freezing-Thawing of Boar Spermatozoa Packaged in Plastic Bags. Journal of Veterinary Medicine Series A. 1994; 41: 37-47
  3. Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C. Levels of Antioxidant Defenses Are Decreased in Bovine Spermatozoa after a Cycle of Freezing and Thawing. Mol Reprod Dev. 2000; 55: 282-8
  4. Nur Z, Zık B, Ustuner B, Sağırkaya H, Ozguden CG. Effects of different cryoprotective agents on ram sperm morphology and DNA integrity. Theriogenology. 2010; 73: 1267-1275
  5. Watson PF. The roles of lipid and protein in the protection of ram spermatozoa at 5 degrees C by egg-yolk lipoprotein. J Reprod Fertil. 1981; 62(2): 483-92
  6. Nur Z, Zik B, Ustuner B, Sagirkaya H, & Ozguden CG. Effects of different cryoprotective agents on ram sperm morphology and DNA integrity. Theriogenology. 2010; 73(9): 1267–1275
  7. Ustuner B, Alcay S, Toker MB, Nur Z, Gokce E, Sonat FA, Soylu MK. Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender. Animal Reproduction Science. 2016; 164: 97–104
  8. Toker MB, Alcay S, Gokce E, Ustuner B. Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience. Cryobiology. 2016; 72; 205–209
  9. Alcay S, Gokce E, Toker MB, Onder NT, Ustuner B, Uzabacı E, Cavus S. Freeze-dried egg yolk based extenders containing various antioxidants improve post thawing quality and incubation resilience of goat spermatozoa. Cryobiology. 2016; 72(3): 269–273
  10. Agarwal A, Prabakaran SA, Said TM. Prevention of oxidative stress injury to sperm. J Androl. 2005; 26: 654-60
  11. Donnelly ET, Mcclure N, Lewis SEM. Glutathione and hypotaurine in vitro: motility, DNA integrity and production of reactive oxygen species. Mutagenesis. 2000; 15: 61-68
  12. Gokce E, Alcay S, Gul Z. Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5°C. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2017; 64: 313–320
  13. Ustuner B, Alcay S, Nur Z, Sagirkaya H, Soylu MK. Effect of egg yolk and soybean lecithin on tris-based e