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Journals / Journal of Anatolian Environmental and Animal Sciences / 2020 / Cilt: 5 - Sayı: 4

Chromatographic Separation Parameters in HPLC Based Pigment Analysis

HPLC Pigment Analizinde Kromatografik Ayırma Parametreleri

Ertuğrul AĞIRBAŞ
Osman AKYÜZ
Pages
570–580
Publication Date
2020-12-31
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Microphytoplankton (8-88%) was the prominent group in the study area, followed by picophytoplankton (3-60%) and nanofitoplankton (1-60%).

📌 3 Key Takeaways

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Objective & Hypothesis:In the present study, chromatographic separation parameters used in pigment analysis and phytoplankton size classes depending on pigments from December 2014 to August 2015 were seasonally investigated along the south-eastern coasts (Giresun-Artvin) of the Black Sea.
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Key Findings & Data:Along the 12 stations, seasonal samplings were collected from surface waters.
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Conclusion & Impact:Microphytoplankton (8-88%) was the prominent group in the study area, followed by picophytoplankton (3-60%) and nanofitoplankton (1-60%).
🔬Design:Research Article
📍Scope:2014 Verileri
⚙️Variables:Fitoplankton · Güney Doğu Karadeniz · HPLC-DAD

Abstract

In the present study, chromatographic separation parameters used in pigment analysis and phytoplankton size classes depending on pigments from December 2014 to August 2015 were seasonally investigated along the south-eastern coasts (Giresun-Artvin) of the Black Sea. Along the 12 stations, seasonal samplings were collected from surface waters. Based on the HPLC-DAD conditions, an appropriate separation of 16 pigment standard was achieved within 38 minutes. The separation parameters and the resolution values showed that the peaks were close to 100%. The used method was validated for pigment analysis. Microphytoplankton (8-88%) was the prominent group in the study area, followed by picophytoplankton (3-60%) and nanofitoplankton (1-60%).

Özet

Güney Doğu Karadeniz (Artvin-Giresun) kıyıları boyunca Kasım 2014-Ağustos 2015 tarihleri arasında mevsimsel olarak yürütülen bu çalışmada pigment analizinde kullanılan kromatorgrafik ayırma parametreleri ve pigmente dayalı fitoplankton boy gruplarının zamansal ve alansal değişimleri araştırılmıştır. Çalışma kapsamında 12 farklı istasyonda yüzeyden mevsimsel örneklemeler gerçekleştirilmiştir. HPLC-DAD koşullarına bağlı olarak gerçekleştirilen analizler sonucunda 16 standart bileşiğin 38 dakika içerisinde uygun ayırımı elde edilmiştir. Ayırma parametreleri ve çözünürlük değerleri piklerin ayırımının %100’e yakın olduğunu göstermiştir. Kullanılan yöntemin pigment analizi için geçereli olduğu görülmüştür. Çalışma bölgesinde mikrofitoplankton (%8-88) öne çıkan grup olurken bunu pikofitoplankton (%3-60) ve nanofitoplankton (%1-60) takip etmiştir.

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Cite This Article

AĞIRBAŞ, E., AKYÜZ, O. (2020). Chromatographic Separation Parameters in HPLC Based Pigment Analysis. Journal of Anatolian Environmental and Animal Sciences, Cilt: 5 - Sayı: 4, 570–580. https://ajindex.com/articles/773615

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