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Journals / International Journal of Innovative Research and Reviews / 2018 / Cilt: 2 - Sayı: 1

Purification and Characterization of a β-Mannanase from Lactobacillus plantarum (ATCC® 14917TM)

Hayrunnisa NADAROGLU
Neslihan DİKBAŞ
Pages
1–6
Publication Date
2018-07-15
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In addition, the effects of some metal ions such as Ca2+, Mn2+, Co2+, Zn2+, Cu2+, Fe2+ and Ni2+ on mannanase enzymes activity was assayed and all the metal ions were increased the enzyme activity from 100 to 344%.

📌 3 Key Takeaways

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Objective & Hypothesis:Mannanase alkaline was purified from thermo Lactobacillus plantarum (ATCC® 14917TM) using ammonium sulfate precipitation and DEAE- Sephadex ion exchange chromatography and Sephacryl S200 Gel filtration chromatography techniques, in 36% yield and 111 -fold.
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Key Findings & Data:Gel filtration chromatography and SDS-PAGE electrophoresis were executed pure enzyme systems 35 kDa and 55 kDa.
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Conclusion & Impact:In addition, the effects of some metal ions such as Ca2+, Mn2+, Co2+, Zn2+, Cu2+, Fe2+ and Ni2+ on mannanase enzymes activity was assayed and all the metal ions were increased the enzyme activity from 100 to 344%.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Mannanase · Lactobacillus plantarum · ATCC® 14917TM

Abstract

Mannanase alkaline was purified from thermo Lactobacillus plantarum (ATCC® 14917TM) using ammonium sulfate precipitation and DEAE- Sephadex ion exchange chromatography and Sephacryl S200 Gel filtration chromatography techniques, in 36% yield and 111 -fold. Gel filtration chromatography and SDS-PAGE electrophoresis were executed pure enzyme systems 35 kDa and 55 kDa. Enzyme subunits were determined as two subunits. Optimum pH 10 and 40 °C was determeined at the optimum temperature. Enzyme activities were retained fairly between 30-80 oC. It was observed that purified mannanase in a wide range was mostly stable at elevated temperatures. In addition, the effects of some metal ions such as Ca2+, Mn2+, Co2+, Zn2+, Cu2+, Fe2+ and Ni2+ on mannanase enzymes activity was assayed and all the metal ions were increased the enzyme activity from 100 to 344%.

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Cite This Article

NADAROGLU, H., DİKBAŞ, N. (2018). Purification and Characterization of a β-Mannanase from Lactobacillus plantarum (ATCC® 14917TM). International Journal of Innovative Research and Reviews, Cilt: 2 - Sayı: 1, 1–6. https://ajindex.com/articles/767591

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