SavedAdd your journal

Journals / International Journal of Agriculture Environment and Food Sciences / 2021 / Cilt: 5 - Sayı: 4

Breaking seed dormancy and regeneration in Cannabis sativa L.

Burak ÖNOL
Mehmet Uğur YILDIRIM
Pages
709–719
Publication Date
2021-12-15
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the best seed germination was noted on Gamborg B5 medium.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Cannabis sativa L. is an important medicinal plant species that grow under natural conditions and has been legalized in 20 out of 81 Turkish provinces.
📊
Key Findings & Data:The study aimed to improve seed germination percentage using various concentrations of GA₃, GA₃ + BAP, germination on water and water solidified with agar, MS or Gamborg B5 medium.
💡
Conclusion & Impact:The results showed that the best seed germination was noted on Gamborg B5 medium.
🔬Design:Research Article
📍Scope:2020 Verileri
⚙️Variables:Hemp · In vitro · mass propagation

Abstract

Cannabis sativa L. is an important medicinal plant species that grow under natural conditions and has been legalized in 20 out of 81 Turkish provinces. The female inflorescence is a highly branched compound raceme with indeterminate habit of growth. This results in different maturing of seeds on the inflorescence and induce physiological dormancy on seeds. The study aimed to improve seed germination percentage using various concentrations of GA₃, GA₃ + BAP, germination on water and water solidified with agar, MS or Gamborg B5 medium. The results showed that the best seed germination was noted on Gamborg B5 medium. Different explants were used to regenerated plantlets on Gamborg B5 medium. All explants were suitable for callus regeneration variably. Only the stem nodes of Samsun Vezirköprü were suitable to induce shoots and plantlets. These plantlets were acclimatized on clay loam soils and transferred to field condition during October 2020, where they acclimatized successfully. These studies provide an effective insight into the mechanism seed dormancy in C. sativa. Further studies using other plant growth regulator concentrations will improve shoot regeneration and aid in utilizing the methods for breeding purpose.

🔖

Cite This Article

ÖNOL, B., YILDIRIM, M. U. (2021). Breaking seed dormancy and regeneration in Cannabis sativa L.. International Journal of Agriculture Environment and Food Sciences, Cilt: 5 - Sayı: 4, 709–719. https://ajindex.com/articles/765213

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (8)

  1. Abrams, D. I. (2016). Integrating cannabis into clinical cancer care. Current Oncology, 23(Suppl 2), S8. DOI: https://doi.org/10.3747/co.23.3099
  2. Adesina, I., Bhowmik, A., Sharma, H., & Shahbazi, A. (2020). A review on the current state of knowledge of growing conditions, agronomic soil health practices and utilities of hemp in the United States. Agriculture, 10(4), 129. DOI: https://doi.org/10.3390/agriculture10040129
  3. Ashraf, M.; Foolad, M.R. (2005). Pre-sowing seed treatment –A shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Advances in Agronomy, 2005, 88, 223–271. DOI: 10.1016/S0065-2113(05)88006-X Aydogan, M., Terzi, Y. E., Gizlenci, S., Mustafa, A. C. A. R., Alpay, E. S. E. N., & Meral, H. (2020). Turkiye’de kenevir yetiştiriciliginin ekonomik olarak yapılabilirligi: Samsun ili Vezirköprü ilcesi ornegi. Anadolu Tarım Bilimleri Dergisi, 35(1), 35-50. DOI: https://doi.org/10.7161/omuanajas.602585
  4. Bello, O. A., Esan, E. B., & Obembe, O. O. (2018). Establishing surface sterilization protocol for nodal culture of Solanecio biafrae. In IOP Conference Series: Earth and Environmental Science, 210(1), 1-5. DOI:10.1088/1755-1315/210/1/012007
  5. Bernstein, N., Gorelick, J., Koch, S. (2019). Interplay between chemistry and morphology in medical cannabis (Cannabis sativa L.). Industrial Crops and Products, 129, 185-194. DOI:https://doi.org/10.1016/j.indcrop.2018.11.039 Blázquez, C., González-Feria, L., Alvarez, L., Haro, L., Casanova, M.LGuzman M. (2004). Cannabinoids inhibit the vascular endothelial growth factor pathway in gliomas. Cancer Research, 64 (16), 5617-23. DOI: https://doi.org/10.1158/0008-5472.CAN-03-3927
  6. Bonini, S. A., Premoli, M., Tambaro, S., Kumar, A., Maccarinelli, G., Memo, M., & Mastinu, A. (2018). Cannabis sativa: A comprehensive ethnopharmacological review of a medicinal plant with a long history. Journal of Ethnopharmacology, 227, 300-315. DOI: https://doi.org/10.1016/j.jep.2018.09.004 Caetano‐Anollés, G., Favelukes, G., & Bauer, W. D. (1990). Optimization of surface sterilization for legume seed. Crop science, 30(3), 708-712. DOI:https://doi.org/10.2135/cropsci1990.0011183X003000030047x
  7. Chahtane, H., Kim, W., & Lopez-Molina, L. (2017). Primary seed dormancy: a temporally multilayered riddle waiting to be unlocked. Journal of Experimental Botany, 68(4), 857-869. DOI: https://doi.org/10.1093/jxb/erw377
  8. Daud, N. H., Jayaraman, S., & Mohamed, R. (2012). Methods Paper: An improved surface